Detection of mutations in gyrB using denaturing high performance liquid chromatography (DHPLC) among Salmonella enterica serovar Typhi and Paratyphi A

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 110, Issue 12, 2016, Pages 684-689

  Gupta, Ruchi ^a,b   Gaind, Rajni ^b   Singh, Laishram Chandreshwor ^c   Paglietti, Bianca ^d   Deb, Monorama ^b   Rubino, Salvatore ^d   Wain, John ^e   Basir, Seemi Farhat ^a  

^a JAMIA MILLIA ISLAMIA CENTRAL UNIVERSITY   (India)

^b SAFDARJUNG HOSPITAL   (India)

^c INDIAN COUNCIL OF MEDICAL RESEARCH   (India)

^d UNIVERSITY OF SASSARI   (Italy)

^e UNIVERSITY OF EAST ANGLIA   (United Kingdom)

Author keywords

Decreased ciprofloxacin susceptibility; DHPLC; GyrB mutation; Salmonella Paratyphi A; Salmonella Typhi

Indexed keywords

CIPROFLOXACIN; DNA TOPOISOMERASE (ATP HYDROLYSING) B; PROTEIN PARC; PROTEIN PARE; ANTIINFECTIVE AGENT; DNA TOPOISOMERASE (ATP HYDROLYSING); QUINOLONE DERIVATIVE;

ARTICLE; CODON; CONTROLLED STUDY; DENATURING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; DRUG SENSITIVITY; EVALUATION STUDY; GENE MUTATION; GENETIC ASSOCIATION; MINIMUM INHIBITORY CONCENTRATION; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; SALMONELLA ENTERICA SEROVAR PARATYPHI A; SALMONELLA ENTERICA SEROVAR TYPHI; SEQUENCE ANALYSIS; ANTIBIOTIC RESISTANCE; DEVICES; DNA MUTATIONAL ANALYSIS; DRUG EFFECTS; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MICROBIAL SENSITIVITY TEST; MISSENSE MUTATION; PROCEDURES;

ANTI-BACTERIAL AGENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA GYRASE; DNA MUTATIONAL ANALYSIS; DRUG RESISTANCE, BACTERIAL; FLUOROQUINOLONES; HUMANS; MICROBIAL SENSITIVITY TESTS; MUTATION, MISSENSE; SALMONELLA PARATYPHI A; SALMONELLA TYPHI;

EID: 85019942260     PISSN: 00359203     EISSN: 18783503     Source Type: Journal    
DOI: 10.1093/trstmh/trx002     Document Type: Article

References (14)

1. WHO. The World Health Report. Shaping the future. Geneva: World Health Organization; 2003
2. Turner AK, Nair S, Wain J. The acquisition of full fluoroquinolone resistance in Salmonella Typhi by accumulation of point mutations in the topoisomerase targets. J Antimicrob Chemother 2006;58:733-40
3. Hopkins KL, Davies RH, Threlfall EJ. Mechanisms of quinolone resistance in Escherichia coli and Salmonella: recent developments. Int J Antimicrob Agents 2005;25:358-73
4. Song Y, Roumangnac P, Weill FX et al. A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A. J Antimicrob Chemother 2010;65:1631-41
5. Walia M, Gaind R, Mehta R et al. Current perspectives of enteric fever: a hospital-based study from India. Ann Trop Paediatr 2005;25: 161-74
6. Abbasi S, Imtiaz A, Usman J et al. Evaluation of the current trend of nalidixic acid susceptibility in typhoidal Salmonellae; a marker of therapeutic failure for the fluoroquinolones. Iran J Microbiol 2011;3:80-3
7. Ngoi ST, Thong KL. High resolution melting analysis for rapid mutation screening in gyrase and topoisomerase IV genes in quinoloneresistant Salmonella enterica. Biomed Res Int 2014;2014:718084:8
8. Le Maréchal C, Audrézet MP, Quéré I et al. Complete and rapid scanning of the cystic fibrosis transmembrane conductance regulator (CFTR) gene by denaturing high-performance liquid chromatography (D-HPLC): major implications for genetic counselling. Hum Genet 2001;108:290-8
9. Sagarzazu NI, Martinez M, Algarra C et al. Optimization of denaturing high performance liquid chromatography technique for rapid detection and identification of acetic acid bacteria of interest in vinegar production. Acetic Acid Bacteria 2013;2:e5
10. Hurtle W, Bode E, Kaplan RS et al. Use of denaturing highperformance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene. J Clin Microbiol 2003;41:4758-66
11. Eaves DJ, Liebana E, Woodward MJ et al. Detection of gyrA mutations in quinolone-resistant Salmonella enterica by denaturing high-performance liquid chromatography. J Clin Microbiol 2002;40: 4121-25
12. Sivakumaran TA, Kucheria K, Oefner PJ. Denaturing high performance liquid chromatography in the molecular diagnosis of genetic disorders. Curr Sci 2003;84:291-6
13. D'Apice MR, Gambardella S, Bengala M et al. Molecular analysis using DHPLC of cystic fibrosis: increase of the mutation detection rate among the affected population in Central Italy. BMC Med Genet 2004;5:8
14. Gaind R, Paglietti B, Murgia M et al. Molecular characterization of ciprofloxacin-resistant Salmonella enterica serovar Typhi and Paratyphi A causing enteric fever in India. J Antimicrob Chemother 2006;58:1139-44