Performance of the Xpert® Carba-R v2 in the daily workflow of a hygiene unit in a country with a low prevalence of carbapenemase-producing Enterobacteriaceae

International Journal of Antimicrobial Agents

Volumn 49, Issue 6, 2017, Pages 774-777

  Hoyos Mallecot, Yannick ^a,b,c   Ouzani, Souad ^a   Dortet, Laurent ^a,b,c,d   Fortineau, Nicolas ^a,b,c,d   Naas, Thierry ^a,b,c,d  

^a BICÊTRE HOSPITAL   (France)

^b Assoc Fr Natl Ref Center for Antibiotic Resistance: Carbapenemase Producing Enterobacteriaceae   (France)

^c Associated French National Reference Center for Antibiotic Resistance: Carbapenemase producing Enterobacteriaceae   (France)

^d INSTITUT PASTEUR   (France)

Author keywords

Carbapenemase producing Enterobacteriaceae; CPE; Infection control; Screening

Indexed keywords

BETA LACTAMASE; BETA LACTAMASE KPC; BETA LACTAMASE NDM; BETA LACTAMASE OXA 48 LIKE; IMIPENEM; TEMOCILLIN; TIMENTIN; UNCLASSIFIED DRUG;

ADULT; AGED; ANTIBIOTIC SENSITIVITY; ARTICLE; CARBAPENEMASE PRODUCING ENTEROBACTERIACEAE; CONTROLLED STUDY; CULTURE MEDIUM; ENRICHMENT CULTURE; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE INFECTION; ESCHERICHIA COLI; FEMALE; HIGH RISK PATIENT; HUMAN; KLEBSIELLA PNEUMONIAE; MAJOR CLINICAL STUDY; MALE; MIDDLE AGED; NONHUMAN; PREDICTIVE VALUE; PREVALENCE; PRIORITY JOURNAL; PROTEUS MIRABILIS; RAPID TEST; SENSITIVITY AND SPECIFICITY; VERY ELDERLY; CARBAPENEM-RESISTANT ENTEROBACTERIACEAE; EVALUATION STUDY; FRANCE; HETEROZYGOTE; INFECTION CONTROL; ISOLATION AND PURIFICATION; MASS SCREENING; MICROBIOLOGICAL EXAMINATION; MOLECULAR DIAGNOSIS; PROCEDURES; PROSPECTIVE STUDY; UNIVERSITY HOSPITAL; WORKFLOW;

ADULT; AGED; AGED, 80 AND OVER; BACTERIOLOGICAL TECHNIQUES; CARBAPENEM-RESISTANT ENTEROBACTERIACEAE; CARRIER STATE; ENTEROBACTERIACEAE INFECTIONS; FEMALE; FRANCE; HOSPITALS, UNIVERSITY; HUMANS; INFECTION CONTROL; MALE; MASS SCREENING; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PREVALENCE; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; WORKFLOW;

EID: 85017502958     PISSN: 09248579     EISSN: 18727913     Source Type: Journal    
DOI: 10.1016/j.ijantimicag.2017.01.025     Document Type: Article

References (18)

1. World Health Organization, Antimicrobial resistance: global report on surveillance 2014. Geneva, Switzerland: WHO; Available from: http://www.who.int/drugresistance/documents/surveillancereport/en/, 2014 [Accessed 18 April 2016].
2. Nordmann, P., Naas, T., Poirel, L., Global spread of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 17 (2011), 1791–1798.
3. Ambler, R.P., Coulson, A.F., Frère, J.M., Ghuysen, J.M., Joris, B., Forsman, M., et al. A standard numbering scheme for the class A β-lactamases. Biochem J 276 (1991), 269–270.
4. Albiger, B., Glasner, C., Struelens, M.J., Grundmann, H., Monnet, D.L., European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group. Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015. Euro Surveill, 20, 2015, 10.2807/1560-7917.ES.2015.20.45.30062.
5. Dortet, L., Cuzon, G., Ponties, V., Nordmann, P., Trends in carbapenemase-producing Enterobacteriaceae, France, 2012 to 2014. Euro Surveill, 22, 2017 pii: 30461.
6. Savard, P., Perl, T.M., Combating the spread of carbapenemases in Enterobacteriaceae: a battle that infection prevention should not lose. Clin Microbiol Infect 20 (2014), 854–861.
7. Fournier, S., Monteil, C., Lepainteur, M., Richard, C., Brun-Buisson, C., Jarlier, V., et al. Long-term control of carbapenemase-producing Enterobacteriaceae at the scale of a large French multihospital institution: a nine-year experience, France, 2004 to 2012. Euro Surveill, 19, 2014 pii: 20802.
8. Viau, R., Frank, K.M., Jacobs, M.R., Wilson, B., Kaye, K., Donskey, C.J., et al. Intestinal carriage of carbapenemase-producing organisms: current status of surveillance methods. Clin Microbiol Rev 29 (2016), 1–27.
9. Schwaber, M.J., Carmeli, Y., An ongoing national intervention to contain the spread of carbapenem-resistant Enterobacteriaceae. Clin Infect Dis 58 (2014), 697–703.
10. ® Carba-R kit for the detection of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 60 (2016), 3832–3837.
11. European Committee on Antimicrobial Susceptibility Testing, Breakpoint tables for interpretation of MICs and zone diameters. Version 5.0; Available from: http://www.eucast.org/ast_of_bacteria/previous_versions_of_documents/, 2015 [Accessed 18 April 2016].
12. Dortet, L., Bréchard, L., Poirel, L., Nordmann, P., Impact of the isolation medium for detection of carbapenemase-producing Enterobacteriaceae using an updated version of the Carba NP test. J Med Microbiol 63 (2014), 772–776.
13. Dortet, L., Cuzon, G., Plésiat, P., Naas, T., Prospective evaluation of an algorithm for the phenotypic screening of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 71 (2016), 135–140.
14. Cuzon, G., Ouanich, J., Gondret, R., Naas, T., Nordmann, P., Outbreak of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae isolates in France. Antimicrob Agents Chemother 55 (2011), 2420–2423.
15. Girlich, D., Nordmann, P., Lécuyer, H., Berche, P., Marmorat-Khuong, A., Gros, I., et al. Multiple colonization with highly resistant bacteria: carbapenemase-producing Enterobacteriaceae, carbapenemase-producing Pseudomonas aeruginosa, carbapenemase-producing Acinetobacter baumannii, and glycopeptide-resistant Enterococcus faecium. Diagn Microbiol Infect Dis 81 (2015), 217–218.
16. Tato, M., Ruiz-Garbajosa, P., Traczewski, M., Dodgson, A., McEwan, A., Humphries, R., et al. Multisite evaluation of Cepheid Xpert Carba-R assay for the detection of carbapenemase-producing organisms in rectal swabs. J Clin Microbiol 54 (2016), 1814–1819.
17. Ruppé, E., Armand-Lefèvre, L., Estellat, C., Consigny, P.-H., El Mniai, A., Boussadia, Y., et al. High rate of acquisition but short duration of carriage of multidrug-resistant Enterobacteriaceae after travel to the tropics. Clin Infect Dis 61 (2015), 593–600.
18. Jousset, A., Bonnin, R., Girlich, D., Cuzon, G., Dortet, L., Glaser, P., et al. Long-term carriage of KPC-producing Klebsiella pneumoniae ST258 over five years. In: 26th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID); 9–12 April 2016; Amsterdam, The Netherlands. Basel, Switzerland: ESCMID, 2016 [P0795].