Generation of gastrointestinal organoids from human pluripotent stem cells

Methods in Molecular Biology

Volumn 1597, Issue , 2017, Pages 167-177

  Múnera, Jorge O ^a   Wells, James M ^a  

^a CINCINNATI CHILDREN S HOSPITAL RESEARCH FOUNDATION   (United States)

Author keywords

Definitive endoderm; Foregut; Gastric organoids; Hindgut; Human pluripotent stem cells; Intestinal organoids

Indexed keywords

CELL DEATH; CELL DIFFERENTIATION; CYTOPLASM; FOREGUT; HUMAN; HUMAN CELL; HUMAN EMBRYONIC STEM CELL; INDUCED PLURIPOTENT STEM CELL; PLURIPOTENT STEM CELL; PRACTICE GUIDELINE; TUMOR SPHEROID; CELL CULTURE TECHNIQUE; CYTOLOGY; ENDODERM; INTESTINE; ORGANOID; PHYSIOLOGY; PROCEDURES; SIGNAL TRANSDUCTION; STOMACH;

CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; ENDODERM; HUMAN EMBRYONIC STEM CELLS; HUMANS; INTESTINES; ORGANOIDS; PLURIPOTENT STEM CELLS; SIGNAL TRANSDUCTION; STOMACH;

EID: 85016978751     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-6949-4_12     Document Type: Chapter

References (6)

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3. Spence JR, Mayhew CN, Rankin SA et al (2011) Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature 470:105-109
4. Wells JM, Spence JR (2014) How to make an intestine. Development 141:752-760
5. D’Amour KA et al (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nat Biotechnol 23:1534-1541. doi:10.1038/nbt1163
6. Sato T et al (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459:262-265. doi:10.1038/nature07935