Carbonyl cyanide m-chlorophenylhydrazine (CCCP) reverses resistance to colistin, but not to Carbapenems and tigecycline in multidrug-resistant Enterobacteriaceae

Frontiers in Microbiology

Volumn 8, Issue FEB, 2017, Pages

  Sekyere, John Osei ^a,b   Amoako, Daniel G ^b  

^a KWAME NKRUMAH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Ghana)

^b UNIVERSITY OF KWAZULU NATAL   (South Africa)

Author keywords

Carbapenem; CCCP; Colistin; Efflux pumps; Protonophore; Tigecycline

Indexed keywords

CARBAPENEM; CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; COLISTIN; TIGECYCLINE;

ANTIBIOTIC SENSITIVITY; ARTICLE; CITROBACTER FREUNDII; CLAVIBACTER MICHIGANENSIS; CONTROLLED STUDY; DISK DIFFUSION; ENTEROBACTERIACEAE; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; SERRATIA MARCESCENS;

EID: 85014428260     PISSN: None     EISSN: 1664302X     Source Type: Journal    
DOI: 10.3389/fmicb.2017.00228     Document Type: Article

References (28)

1. Clinical Laboratory Standards Institute (CLSI) (2009). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Eigth Edition. Broth Microdilution Method. Wayne, PA: CLSI.
2. European Committee on Antimicrobial Susceptibility Testing (2016). Breakpoint Tables for Interpretation of MICs and Zone Diameters. Version 6.0, 2016. 1-78. Available online at: http://www.eucast.org.
3. He, F., Fu, Y., Chen, Q., Ruan, Z., Hua, X., Zhou, H. et al. (2015). Tigecycline susceptibility and the role of efflux pumps in tigecycline resistance in KPC-producing Klebsiella pneumoniae. PLoS ONE 10:e0119064. doi: 10.1371/journal.pone.0119064.
4. Huang, L., Sun, L., Xu, G., and Xia, T. (2008). Differential susceptibility to carbapenems due to the AdeBC efflux pump among nosocomial outbreak isolates of Acinetobacter baumanii in a Chinese hospital. Diagn. Microbiol. Infect. Dis. 62, 326-332. doi: 10.1016/j.diagmicrobio.2008.06.008.
5. Li, X. Z., Plesiat, P., and Nikaido, H. (2015). The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria. Clin. Microbiol. Rev. 28, 337-418. doi: 10.1128/CMR.00117-14.
6. Mohamed, Y. F., Abou-Shleib, H. M., Khalil, A. M., El-Guink, N. M., and El-Nakeeb, M. A. (2016). Membrane permeabilization of colistin toward pan-drug resistant Gram-negative isolates. Braz. J. Microbiol. 47, 381-388. doi: 10.1016/j.bjm.2016.01.007.
7. Ni, W., Li, Y., Guan, J., Zhao, J., Cui, J., Wang, R., et al. (2016). Effects of Efflux Pump Inhibitors on Colistin Resistance in Multidrug-Resistant Gram-Negative Bacteria. Antimicrob. Agents Chemother. 60, 3215-3218. doi: 10.1128/AAC.00248-16.
8. Olaitan, A. O., Morand, S., and Rolain, J.-M. (2014). Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front. Microbiol. 5:643. doi: 10.3389/fmicb.2014.00643.
9. Opperman, T. J., and Nguyen, S. T. (2015). Recent advances toward a molecular mechanism of efflux pump inhibition. Front. Microbiol. 6:421. doi: 10.3389/fmicb.2015.00421.
10. Osei Sekyere, J. (2016). Current state of resistance to Antibiotics of Last-Resort in South Africa: a review from a public health perspective. Front. Public Heal. 4:209. doi: 10.3389/fpubh.2016.00209.
11. Osei Sekyere, J., Pedersen, T., Sivertsen, A., Govinden, U., Essack, S. Y., Moodley, K., et al. (2016c). "Molecular epidemiology of carbapenem, colistin and tigecycline resistant Enterobacteriaceae," in ECCMID Poster Presentation, (Amsterdam).
12. Osei Sekyere, J., Govinden, U., and Essack, S. Y. (2015). Review of established and innovative detection methods for carbapenemase-producing Gram-negative bacteria. J. Appl. Microbiol. 119, 1219-1233. doi: 10.1111/jam.12918.
13. Osei Sekyere, J., Govinden, U., and Essack, S. Y. (2016a). Comparison of existing phenotypic and genotypic tests for the detection of NDM and GES carbapenemase-producing Enterobacteriaceae. J. Pure Appl. Microb. 10, 2585-2591. doi: 10.22207/JPAM.10.4.14.
14. Osei Sekyere, J., Govinden, U., Bester, L. A., and Essack, S. Y. (2016b). Colistin and tigecycline resistance in carbapenemase-producing gram negative bacteria: emerging resistance mechanisms and detection methods. J. Appl. Microbiol. 121, 601-617. doi: 10.1111/jam.13169.
15. Park, Y. K., and Ko, K. S. (2015). Effect of carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on killing Acinetobacter baumannii by colistin. J. Microbiol. 53, 53-59. doi: 10.1007/s12275-015-4498-5.
16. Patel, G., and Bonomo, R. A. (2013). 'Stormy waters ahead': global emergence of carbapenemases. Front. Microbiol. 4:48. doi: 10.3389/fmicb.2013.00048.
17. Pournaras, S., Koumaki, V., Spanakis, N., Gennimata, V., and Tsakris, A. (2016). Current perspectives on tigecycline resistance in Enterobacteriaceae: susceptibility testing issues and mechanisms of resistance. Int. J. Antimicrob. Agents 48, 11-18. doi: 10.1016/j.ijantimicag.2016.04.017.
18. Pragasam, A. K., Sahni, R. D., Anandan, S., Sharma, A., Gopi, R., Hadibasha, N., et al. (2016). A pilot study on carbapenemase detection: do we see the same level of agreement as with the CLSI observations. J. Clin. Diagn. Res. 10, DC09-DC13. doi: 10.7860/jcdr/2016/16417.8152.
19. Pragasam, A. K., Shankar, C., Veeraraghavan, B., Biswas, I., Nabarro, L. E. B., Inbanathan, F. Y., et al. (2017). Molecular mechanisms of colistin resistance in Klebsiella pneumoniae Causing Bacteremia from India-a first report. Front. Microbiol. 7:2135. doi: 10.3389/fmicb.2016.02135.
20. Pule, C. M., Sampson, S. L., Warren, R. M., Black, P. A., van Helden, P. D., Victor, T. C., et al. (2016). Efflux pump inhibitors: targeting mycobacterial efflux systems to enhance TB therapy. J. Antimicrob. Chemother. 71, 17-26. doi: 10.1093/jac/dkv316.
21. Radchenko, M., Symersky, J., Nie, R., and Lu, M. (2015). Structural basis for the blockade of MATE multidrug efflux pumps. Nat. Commun. 6:7995. doi: 10.1038/ncomms8995.
22. Ribera, A., Jurado, A., Ruiz, J., Marco, F., Del Valle, O., Mensa, J., et al. (2002). In vitro activity of clinafloxacin in comparison with other quinolones against Stenotrophomonas maltophilia clinical isolates in the presence and absence of reserpine. Diagn. Microbiol. Infect. Dis. 42, 123-128. doi: 10.1016/s0732-8893(01)00335-2.
23. Ricci, V., Blair, J. M. A., and Piddock, L. J. V. (2014). RamA, which controls expression of the MDR efflux pump AcrAB-TolC, is regulated by the Lon protease. J. Antimicrob. Chemother. 69, 643-650. doi: 10.1093/jac/dkt432.
24. Sekyere, J. O., Govinden, U., and Essack, S. (2015). The molecular epidemiology and genetic environment of carbapenemases detected in Africa. Microb. Drug Resist. 22, 59-68. doi: 10.1089/mdr.2015.0053.
25. Shinabarger, D. L., Zurenko, G. E., Hesje, C. K., Sanfilippo, C. M., Morris, T. W., and Haas, W. (2011). Evaluation of the effect of bacterial efflux pumps on the antibacterial activity of the novel fluoroquinolone besifloxacin. J. Chemother. 23, 80-86. doi: 10.1179/joc.2011.23.2.80.
26. Spindler, E. C., Hale, J. D. F., Giddings, T. H., Hancock, R. E. W., and Gill, R. T. (2011). Deciphering the mode of action of the synthetic antimicrobial peptide Bac8c. Antimicrob. Agents Chemother. 55, 1706-1716. doi: 10.1128/AAC.01053-10.
27. Surendranath, C. R., Ramana, B. V., Chaudhury, A., and Gururaj Kumar, A. (2014). Functional study of efflux pump inhibitors in clinical isolates of multidrug resistant Klebsiella pneumoniae. Asian J. Pharm. Clin. Res. 7, 80-82.
28. Yu, Z., Cai, Y., Qin, W., Lin, J., and Qiu, J. (2015). Polymyxin E Induces Rapid Paenibacillus polymyxa Death by Damaging cell membrane while Ca2+ can protect cells from damage. PLoS ONE 10:e0135198. doi: 10.1371/journal.pone.0135198.