Clinical validation of 3 commercial real-time reverse transcriptase polymerase chain reaction assays for the detection of Middle East respiratory syndrome coronavirus from upper respiratory tract specimens

Diagnostic Microbiology and Infectious Disease

Volumn 87, Issue 4, 2017, Pages 320-324

  Mohamed, Deqa H ^a   AlHetheel, AbdulKarim F ^a   Mohamud, Hanat S ^a   Aldosari, Kamel ^b   Alzamil, Fahad A ^a   Somily, Ali M ^a  

^a KING SAUD UNIVERSITY   (Saudi Arabia)

^b Pathology Department   (Saudi Arabia)

Author keywords

MERS CoV; Orf1a; RT PCR; Saudi Arabia; UpE

Indexed keywords

ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; GOLD STANDARD; HUMAN; MIDDLE EAST RESPIRATORY SYNDROME; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; NONHUMAN; POLYMERASE CHAIN REACTION SYSTEM; PREDICTIVE VALUE; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SAUDI ARABIA; SENSITIVITY AND SPECIFICITY; UPPER RESPIRATORY TRACT; VALIDATION STUDY; VIRUS DETECTION; COMPARATIVE STUDY; CORONAVIRUS INFECTION; ENGLAND; EPIDEMIC; GENETICS; GERMANY; MICROBIOLOGY; PROCEDURES; RESPIRATORY SYSTEM; RESPIRATORY TRACT INFECTION; SOUTH KOREA;

CORONAVIRUS INFECTIONS; DISEASE OUTBREAKS; ENGLAND; GERMANY; HUMANS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REPUBLIC OF KOREA; RESPIRATORY SYSTEM; RESPIRATORY TRACT INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SAUDI ARABIA;

EID: 85013158213     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2017.01.003     Document Type: Article

References (10)

1. Corman, V.M., Eckerle, I., Bleicker, T., Zaki, A., Landt, O., Eschbach-Bludau, M., et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill, 17(39), 2012, 20285.
2. Corman, V.M., Müller, M.A., Costabel, U., Timm, J., Binger, T., Meyer, B., et al. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill, 17(49), 2012, 20334.
3. Corman, V.M., Ölschläger, S., Wendtner, C.M., Drexler, J.F., Hess, M., Drosten, C., Performance and clinical validation of the RealStar® MERS-CoV kit for detection of Middle East respiratory syndrome coronavirus RNA. J Clin Virol 60:2 (2014), 168–171.
4. Kim, M.N., Ko, Y.J., Seong, M.W., Kim, J.S., Shin, B.M., Sung, H., Analytical and clinical validation of six commercial Middle East respiratory syndrome coronavirus RNA detection kits based on real-time reverse-transcription PCR. Ann Lab Med 36:5 (2016), 450–456.
5. Lu, X., Whitaker, B., Sakthivel, S.K., Kamili, S., Rose, L.E., Lowe, L., et al. Real-time reverse transcription-PCR assay panel for Middle East respiratory syndrome coronavirus. J Clin Microbiol 52:1 (2014), 67–75.
6. Pas, S.D., Patel, P., Reusken, C., Domingo, C., Corman, V.M., Drosten, C., et al. First international external quality assessment of molecular diagnostics for MERS-CoV. J Clin Virol 69 (2015), 81–85.
7. Somily, A., Barry, M., Al Subaie, S., BinSaeed, A., Alzamil, F., Zaher, W., et al. New MERS-CoV sequences Feb-Mar 2015 and preliminary analysis MERS coronavirus. Dataset available at: http://virological.org/t/new-mers-cov-sequences-feb-mar-2015-and-preliminary-analysis/140 [Accessed 7 January 2016].
8. van Boheemen, S., de Graaf, M., Lauber, C., Bestebroer, T.M., Raj, V.S., Zaki, A.M., et al. Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. MBio, 3(6), 2012 [pii: e00473–12].
9. Whitaker, B., Sakthivel, S., Lu, X., Erdman, D., Evaluation of 3 commercial real-time RT-PCR assays for Middle East respiratory syndrome coronavirus. Poster. Available at: http://www.fast-trackdiagnostics.com/media/218346/mers-kit-comparison-cvs-poster-2015.pdf.
10. Zaki, A.M., van Boheemen, S., Bestebroer, T.M., Osterhaus, A.D., Fouchier, R.A., Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 367:19 (2012), 1814–1820.