Detection of Viral RNA in Tissues following Plasma Clearance from an Ebola Virus Infected Patient

PLoS Pathogens

Volumn 13, Issue 1, 2017, Pages

  Biava, Mirella ^a   Caglioti, Claudia ^a   Bordi, Licia ^a   Castilletti, Concetta ^a   Colavita, Francesca ^a   Quartu, Serena ^a   Nicastri, Emanuele ^a   Lauria, Francesco Nicola ^a,b   Petrosillo, Nicola ^a   Lanini, Simone ^a,b   Hoenen, Thomas ^c   Kobinger, Gary ^b,d   Zumla, Alimuddin ^b,e   Di Caro, Antonino ^a,b   Ippolito, Giuseppe ^a,b   Capobianchi, Maria Rosaria ^a   Lalle, Eleonora ^a  

^a NATIONAL INSTITUTE FOR INFECTIOUS DISEASES L SPALLANZANI   (Italy)

^b International Public Health Crisis Group (IPHCG)   (United States)

^c FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^d LAVAL UNIVERSITY   (Canada)

^e UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC RNA; MESSENGER RNA; VIRUS RNA;

ARTICLE; EBOLA HEMORRHAGIC FEVER; EBOLAVIRUS; HEALTH CARE PERSONNEL; HUMAN; HUMAN TISSUE; NONHUMAN; PLASMA CLEARANCE; REAL TIME POLYMERASE CHAIN REACTION; RNA EXTRACTION; SPUTUM ANALYSIS; THROAT CULTURE; URINE SAMPLING; VIRAL CLEARANCE; VIRUS REPLICATION; VIRUS SHEDDING; VIRUS TRANSMISSION; GENETICS; POLYMERASE CHAIN REACTION; VIROLOGY;

EBOLAVIRUS; HEMORRHAGIC FEVER, EBOLA; HUMANS; POLYMERASE CHAIN REACTION; RNA, VIRAL;

EID: 85010966277     PISSN: 15537366     EISSN: 15537374     Source Type: Journal    
DOI: 10.1371/journal.ppat.1006065     Document Type: Article

References (20)

1. Dye C, WHO Ebola Response Team. Ebola virus disease in Western Africa–the first 9 months. N Engl J Med. 2015; 372(2):189.
2. Bevilacqua N, Nicastri E, Chinello P, Puro V, Petrosillo N, Di Caro A, Capobianchi MR, Lanini S, Vairo F, Pletschette M, Zumla A, Ippolito G; INMI Ebola Team. Criteria for discharge of patients with Ebola virus diseases in high-income countries. Lancet Glob Health. 2015Dec;3(12):e739–40. doi: 10.1016/S2214-109X(15)00205-326608193
3. Castilletti C, Carletti F, Gruber CE, Bordi L, Lalle E, Quartu S, Meschi S, et al. Molecular Characterization of the First Ebola Virus Isolated in Italy, from a Health Care Worker Repatriated from Sierra Leone. Genome Announce. 2015; 3.
4. Petrosillo N, Nicastri E, Lanini S, Capobianchi MR, Di Caro A, Antonini M, Puro V, et al. Ebola virus disease complicated with viral interstitial pneumonia: a case report. BMC Infect Dis. 2015; 15:432. doi: 10.1186/s12879-015-1169-426471197
5. Liddell AM, Davey RT, JrMehta AK, Varkey JB, Kraft CS, Tseggay GK, Badidi O, et al. Characteristics and Clinical Management of a Cluster of 3 Patients With Ebola Virus Disease, Including the First Domestically Acquired Cases in the United States. Ann Intern Med. 2015; 163:81–90. doi: 10.7326/M15-053025961438
6. Osterholm MT, Moore KA, Kelley NS, Brosseau LM, Wong G, Murphy FA, Peters CJ, et al. Transmission of Ebola viruses: what we know and what we do not know. MBio. 2015; 6:e00137. doi: 10.1128/mBio.00137-1525698835
7. Agrati C, Castilletti C, Casetti R, Sacchi A, Falasca L, Turchi F, Tumino N, et alLongitudinal characterization of dysfunctional T cell-activation during human acute Ebola infection. Cell Death Dis. 2016Mar31;7:e2164. doi: 10.1038/cddis.2016.5527031961
8. Twenhafel NA, Shaia CI, Bunton TE, Shamblin JD, Wollen SE, Pitt LM, Sizemore DR, Ogg MM, Johnston SC, Experimental aerosolized guinea pig-adapted Zaire ebolavirus (variant: Mayinga) causes lethal pneumonia in guinea pigs. Vet Pathol. 2015Jan;52(1):21–5. doi: 10.1177/030098581453561224829285
9. Johnson E, Jaax N, White J, Jahrling P, Lethal experimental infections of rhesus monkeys by aerosolized Ebola virus. Int J Exp Pathol. 1995Aug;76(4):227–36. 7547435
10. Twenhafel NA, Mattix ME, Johnson JC, Robinson CG, Pratt WD, Cashman KA, Wahl-Jensen V, Terry C, Olinger GG, Hensley LE, Honko AN, Pathology of experimental aerosol Zaire ebolavirus infection in rhesus macaques. Vet Pathol. 2013May;50(3):514–29doi: 10.1177/030098581246963623262834
11. Weingartl HM, Embury-Hyatt C, Nfon C, Leung A, Smith G, Kobinger G, Transmission of Ebola virus from pigs to non-human primates. Sci Rep. 2012;2:811. doi: 10.1038/srep0081123155478
12. Martines RB, Ng DL, Greer PW, Rollin PE, Zaki SR, Tissue and cellular tropism, pathology and pathogenesis of Ebola and Marburg viruses. J Pathol. 2015;235(2):153–74doi: 10.1002/path.445625297522
13. Hoenen T, Jung S, Herwig A, Groseth A, Becker S, Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription. Virology. 2010; 403:56–66. doi: 10.1016/j.virol.2010.04.00220444481
14. Shabman RS, Hoenen T, Groseth A, Jabado O, Binning JM, Amarasinghe GK, Feldmann H, et al. An upstream open reading frame modulates ebola virus polymerase translation and virus replication. PLoS Pathog. 2013; 9:e1003147. doi: 10.1371/journal.ppat.100314723382680
15. WHO, 2009. CDC protocol of real-time RT-PCR for influenza A (H1N1). http://www.who.int/csr/resources/publications/swineflu/realtimeptpcr/en/ index.html
16. Janvier F, Delaune D, Poyot T, Valade E, Mérens A, Rollin PE, Foissaud V, Ebola Virus RNA Stability in Human Blood and Urine in West Africa's Environmental Conditions. Emerg Infect Dis. 2016; 22:292–4. doi: 10.3201/eid2202.15139526812135
17. Wong G, Qiu X, de La Vega MA, Fernando L, Wei H, Bello A, Fausther-Bovendo H, Audet J, Kroeker A, Kozak R, Tran K, He S, Tierney K, Soule G, Moffat E, Günther S, Gao GF, Strong J, Embury-Hyatt C, Kobinger G, Pathogenicity Comparison Between the Kikwit and Makona Ebola Virus Variants in Rhesus Macaques. J Infect Dis. 2016Sep19.
18. Kobinger GP, Leung A, Neufeld J, Richardson JS, Falzarano D, Smith G, Tierney K, Patel A, Weingartl HM, Replication, pathogenicity, shedding, and transmission of Zaire ebolavirus in pigs. J Infect Dis. 2011Jul15;204(2):200–8. doi: 10.1093/infdis/jir07721571728
19. Fang LQ, Yang Y, Jiang JF, Yao HW, Kargbo D, Li XL, Jiang BG, Kargbo B et al. Transmission dynamics of Ebola virus disease and intervention effectiveness in Sierra Leone PNAS 2016, published ahead of print March 28, 2016
20. Crozier I, Ebola Virus RNA in the Semen of Male Survivors of Ebola Virus Disease: The Uncertain Gravitas of a Privileged Persistence. J Infect Dis. 2016May3.