Comparison of DNA quantification methodology used in the DNA extraction protocol for the UK Biobank cohort

BMC Genomics

Volumn 18, Issue 1, 2017, Pages

  Welsh, Samantha ^a   Peakman, Tim ^a   Sheard, Simon ^a   Almond, Rachael ^a  

^a UK BIOBANK   (United Kingdom)

Author keywords

Affymetrix; DNA concentration; Genotyping; PicoGreen; Quantification; Trinean; UK Biobank; UV Vis

Indexed keywords

DNA;

ALGORITHM; ARTICLE; COHORT ANALYSIS; DNA EXTRACTION; GENOTYPE; INTERMETHOD COMPARISON; LABORATORY; ULTRAVIOLET SPECTROSCOPY; UNITED KINGDOM; BIOBANK; GENOTYPING TECHNIQUE; HUMAN; ISOLATION AND PURIFICATION; PROCEDURES; SPECIMEN HANDLING; STANDARDS;

ALGORITHMS; BIOLOGICAL SPECIMEN BANKS; DNA; GENOTYPING TECHNIQUES; HUMANS; SPECIMEN HANDLING; UNITED KINGDOM;

EID: 85008239366     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/s12864-016-3391-x     Document Type: Article

References (17)

1. Ollier W, Sprosen T, Peakman T. UK biobank: from concept to reality. Pharmacogenomics. 2005;6(6):639-46.
2. Allen NE, Sudlow C, Peakman T, Collins R, Biobank UK. UK biobank data: come and get it. Sci transl med. 2014;6:224. United States.
3. Elliott P, Peakman TC. The UK biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine. Int j epidemiol. 2008;37(2):234-44.
4. Peakman TC, Elliott P. The UK biobank sample handling and storage validation studies. Int j epidemiol. 2008;37 Suppl 1:i2-6.
5. Sudlow C, Gallacher J, Allen N, Beral V, Burton P, Danesh J, Downey P, Elliott P, Green J, Landray M, et al. UK biobank: an open access resource for identifying the causes of a wide range of complex diseases of middle and old age. Plos med. 2015;12(3):e1001779.
6. UK Biobank Axiom® Array-Content Summary [http://media.affymetrix.com/support/technical/brochures/uk_axiom_biobank_contentsummary_brochure.pdf?cid=2014070005]. Accessed 16 Nov 2015.
7. Wain LV, Shrine N, Miller S, Jackson VE, Ntalla I, Artigas MS, Billington CK, Kheirallah AK, Allen R, Cook JP, et al. Novel insights into the genetics of smoking behaviour, lung function, and chronic obstructive pulmonary disease (UK BiLEVE): a genetic association study in UK biobank. Lancet respir med. 2015;3(10):769-81.
8. Haque KA, Pfeiffer RM, Beerman MB, Struewing JP, Chanock SJ, Bergen AW. Performance of high-throughput DNA quantification methods. BMC biotechnol. 2003;3:20.
9. Nicklas JA, Buel E. Quantification of DNA in forensic samples. Anal bioanal chem. 2003;376(8):1160-7.
10. Nielsen K, Mogensen HS, Hedman J, Niederstatter H, Parson W, Morling N. Comparison of six DNA quantification methods. Int Congr Ser. 2006;1288:759-61.
11. Nielsen K, Mogensen HS, Hedman J, Niederstatter H, Parson W, Morling N. Comparison of five DNA quantification methods. Forensic sci int genet. 2008;2(3):226-30.
12. Blood DNA extraction and DNA normalization. [http://lifesciences.tecan.com/applications_and_solutions/genomics/nucleid_acid_purification]. Accessed 9 Dec 2016.
13. Axiom® 2.0 Assay Automated Workflow, P/N 702963 Rev 3. [http://media.affymetrix.com/support/downloads/manuals/axiom_2_assay_auto_workflow_user_guide.pdf]. Accessed 12 Nov 2015.
14. UKB_WCSGAX: UK Biobank 500 K Samples Genotyping Data Generation by the Affymetrix Research Services Laboratory. [http://biobank.ctsu.ox.ac.uk/crystal/docs/genotyping_affy_generation.pdf]. Accessed 12 Nov 2015.
15. Analysis Guide - Axiom® Genotyping Solution Data Analysis Guide, P/N 702961 Rev. 3. [http://media.affymetrix.com/support/downloads/manuals/axiom_genotyping_solution_analysis_guide.pdf]. Accessed 12 Nov 2015.
16. Genotyping and quality control of UK Biobank, a large-scale, extensivelt phenotyped prospective resource - Information for researchers - Interim Data Release, 2015. [http://biobank.ctsu.ox.ac.uk/crystal/docs/genotyping_qc.pdf]. Accessed 12 Nov 2015.
17. Handling Fresh or Frozen Clumpy Samples with DNAse I. [http://www.humanimmunologyportal.com/wp-content/uploads/pdf/singles/handling-fresh-or-frozen-clumpy-samples-with-dnase-i.pdf]. Accessed 1 Dec 2015.