Measurement of refractive index by nanoparticle tracking analysis reveals heterogeneity in extracellular vesicles

Journal of Extracellular Vesicles

Volumn 3, Issue 1, 2014, Pages

  Gardiner, Chris ^a   Shaw, Michael ^a   Hole, Patrick ^b   Smith, Jonathan ^b   Tannetta, Dionne ^a   Redman, Christopher W ^a   Sargent, Ian L ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b MALVERN INSTRUMENTS LTD   (United Kingdom)

Author keywords

Extracellular vesicles; Light scattering; Nanoparticle tracking analysis; Refractive index; Standardisation

Indexed keywords

MICROSPHERE; POLYSTYRENE;

ANALYTIC METHOD; ARTICLE; BLOOD; EXOSOME; LIGHT SCATTERING; LYOPHILISATE; MICROSCOPY; NANOPARTICLE TRACKING ANALYSIS; NEUROBLASTOMA; PARTICLE SIZE; PHYSICAL CHEMISTRY; PHYSICAL PARAMETERS; PLACENTA; REFRACTION INDEX; THROMBOCYTE; URINE;

EID: 85006957901     PISSN: None     EISSN: 20013078     Source Type: Journal    
DOI: 10.3402/jev.v3.25361     Document Type: Article

References (17)

1. Van Der Pol E, Hoekstra AG, Sturk A, Otto C, Van Leeuwen TG, Nieuwland R. Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost. 2010;8:2596-607.
2. Gardiner C, Ferreira YJ, Dragovic RA, Redman CWG, Sargent IL. Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles. 2013;2: 19671, doi: http://dx.doi.org/10.3402/jev.v2i0.19671.
3. Chandler WL, Yeung W, Tait JF. A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost. 2011;9: 1216-24.
4. Lacroix R, Robert S, Poncelet P, Kasthuri RS, Key NS, Dignat-George F. Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. J Thromb Haemost. 2010;8: 2571-4.
5. Robert S, Poncelet P, Lacroix R, Arnaud L, Giraudo L, Hauchard A, et al. Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies? J Thromb Haemost. 2009;7:190-7.
6. Beuthan J, Minet O, Helfmann J, Herrig M, Muller G. The spatial variation of the refractive index in biological cells. Phys Med Biol. 1996;41:369-82.
7. Tannetta DS, Dragovic RA, Gardiner C, Redman CW, Sargent IL. Characterisation of syncytiotrophoblast vesicles in normal pregnancy and pre-eclampsia: expression of Flt-1 and endoglin. PLoS One. 2013;8:e56754.
8. Pena O, Pal U. Scattering of electromagnetic radiation by a multilayered sphere. Comput Phys Comm. 2009;180:2348-54.
9. Kiefer J. Sequential minimax search for a maximum. Proc Am Math Soc. 1953;4:6.
10. Bohren CF, Huffman DR. Absorption and scattering by a sphere. In: Bohren CF, Huffman DR, editors. Absorption and scattering of light by small particles. Weinheim: Wiley-VCH Verlag GmbH; 2007. p. 83-129.
11. Webber J, Clayton A. How pure are your vesicles? J Extracell Vesicles. 2013;2:19861, doi: http://dx.doi.org/10.3402/jev.v2i0. 19861.
12. Redman CW, Tannetta DS, Dragovic RA, Gardiner C, Southcombe JH, Collett GP, et al. Review: does size matter? Placental debris and the pathophysiology of pre-eclampsia. Placenta. 2012;33(Suppl):S48-54.
13. Konokhova AI, Yurkin MA, Moskalensky AE, Chernyshev AV, Tsvetovskaya GA, Chikova ED, et al. Light-scattering flow cytometry for identification and characterization of blood microparticles. J Biomed Opt. 2012;17:057006.
14. van der Pol E, Coumans FA, Sturk A, Nieuwland R, Van Leeuwen TG. Refractive index determination of nanoparticles in suspension using nanoparticle tracking analysis. Nano letters. 2014;14:6195-201.
15. Aleman MM, Gardiner C, Harrison P, Wolberg AS. Differential contributions of monocyte-and platelet-derived microparticles towards thrombin generation and fibrin formation and stability. J Thromb Haemost. 2011;9:2251-61.
16. Gardiner C, Dragovic RA, Brooks AS, Tannetta DS, Ferguson DJ, Hole P, et al. Sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis. Nanomedicine. 2011;7:780-8.
17. Huang C, Thompson TE. Properties of lipid bilayer membranes separating two aqueous phases: determination of membrane thickness. J Mol Biol. 1965;13:183-93.