Quantification of ergosterol and ergosterol peroxide in several medicinal fungi by high performance liquid chromatography monitored with a diode array detection-atmospheric pressure chemical ionization-ion trap mass spectrometer

Journal of Traditional Medicines

Volumn 25, Issue 1, 2008, Pages 18-23

  Lee, Joo Sang ^a   Ma, Chao Mei ^a   Hattori, Masao ^a  

^a UNIVERSITY OF TOYAMA   (Japan)

Author keywords

ergosterol; ergosterol peroxide; HPLC DAD APCI MS; medicinal mushroom; quantitative analysis

Indexed keywords

EID: 84992652416     PISSN: 18801447     EISSN: 18813747     Source Type: Journal    
DOI: 10.11339/jtm.25.18     Document Type: Article

References (22)

1. Czub, J., Baginski, M. J.: Comparative molecular dynamics study of lipid membranes containing cholesterol and ergosterol. Biophys. J., 90, 2368-2382, 2006.
2. Suárez, Y., Fernández, C., Ledo, B., Ferruelo, A. J., Martín, M., Vega, M. A. Gómez-Coronado, D., Lasunción, M. A.: Differential effects of ergosterol and cholesterol on Cdk1 activation and SRE-driven transcription. Sterol stringency for cell cycle progression in human cells. Eur. J. Biochem., 269, 1761-1771, 2002.
3. Takaku, T., Kimura, Y., Okuda, H.: Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action J. Nutr., 131, 1409-1413, 2001.
4. Ponce, M. A., Ramirez, J. A., Galagovsky, L. R., Gros, E. G., Erra-Balsells, R.: A new look into the reaction between ergosterol and singlet oxygen in vitro. Photochem. Photobiol. Sci., 1, 749-756, 2002.
5. Slominski, A., Semak, I., Zjawiony, J., Wortsman, J., Gandy, M. N., Li, J., Zbytek, B., Li, W., Tuckey, R. C.: Enzymatic metabolism of ergosterol by cytochrome p450scc to biologically active 17α, 24-dihydroxyergosterol. Chemistry and Biology, 12, 931-939, 2005.
6. Gunatilaka, A. A. L., Gopichand, Y., Schmitz, F. J., Djerassi, C.: Minor and trace sterols in marine invertebrates, 26: Isolation and structure elucidation of nine new 5-alpha, 8-alpha-epidioxy sterols from four marine organisms. Can. J. Org. Chem., 46, 3860-3866, 1981.
7. Tchouankeu, J. C., Nyasse, B., Tsamo, E., Sondengam, B. L., Morin, C.: An ergostane derivative from the bark of Entandrophragma utile. Phytochemistry, 31, 704-705, 1992.
8. Adam, H. K., Campbell, I. M., McCorkindale, N. J.: Ergosterol peroxi-de: a fungal artifact. Nature, 216, October 28, 397, 1967.
9. Sgarbi, D. B., da Silva, A. J., Carlos, I. Z., Angluster, J., Alviano, C. S.: Isolation of ergosterol peroxide and its reversion to ergosterol in the pathogenic fungus Sporothrix schenckii. Mycopathologia, 139, 9-14, 1997.
10. Jong, S. C., Donovick, R.: Antitumor and antiviral substances produced from fungi. Adv. Appl. Microbiol. 34, 183-262, 1989.
11. Nam, K. S., Jo, Y. S., Kim, Y. H., Hyun, J. W., Kim, H. W.: Cytotoxic activities of acetoxyscirpenediol and ergosterol peroxide from Paecilomyces tenuipes. Life Sciences, 69, 229-237, 2001.
12. Bok, J. W., Lermer, L., Chilton, J., Klingeman, H. G., Neil Towers, G. H.: Antitumor sterols from the mycelia of Cordyceps sinensis. Phytochemistry, 51, 891-898, 1999.
13. Kahlos, K., Kangas, L., Hiltunen, R.: Ergosterol peroxide, an active compound from Inonotus radiatus. Planta Med., 55, 389-390, 1989.
14. Kwon, H. C., Zee, S. D., Cho, S. Y., Choi, S. U., Lee, K. R.: Cytotoxic ergosterols from Paecilomyces sp. J300. Arch. Pharm. Res. 25, 851-855, 2002.
15. Subbiah, M. T. R., Abplanalp, W.: Ergosterol (major sterol of baker's and brewer's yeast extracts) inhibits the growth of human breast cancer cells in vitro and the potential role of its oxidation products. Int. J. Vitam. Nutr. Res., 73, 19-23, 2003.
16. Yazawa, Y., Yokota, M., Sugiyama, K.: Antitumor promoting effect of an active component of polyporus, ergosterol and related compounds on rat urinary bladder carcinogenesis in a short-term test with concanavalin A. Biol. Pharm. Bull., 23, 1298-1302, 2000.
17. Yuan, J. P., Wang, J. H., Liu, X.: Distribution of free and esterified ergosterols in the medicinal fungus Ganoderma lucidum. Appl. Microbial. Biotechnol., 77, 159-165, 2007.
18. Yuan, J. P., Wang, J. H., Liu, X., Kuang, H. C., Zhao, S. Y.: Simultaneous determination of free ergosterol and ergosteryl esters in Cordyceps sinensis by HPLC. Food Chemistry, 105, 1755-1759, 2007.
19. Dong, Y., Steffenson, B. J., Mirocha, C. J.: Analysis of ergosterol in single kernel and ground grain by gas chromatography-mass spectrometry. J. Agric. Food Chem., 54, 4121-4125, 2006.
20. Böcking, T., Barrow, K. D., Nettinga, G., Chilcott, T. C., Coster, H. G. L., Höfer, M.: Effects of singlet oxygen on membrane sterols in the yeast Saccharomyces cerevisiae. Eur. J. Biochem., 267, 1607-1618, 2000.
21. Lee, J. S., Ma, C. M., Park, D. K., Yoshimi, Y., Hatanaka, M., Hattori, M.: Transformation of ergosterol peroxide to cytotoxic substances by rat intestinal bacteria. Biol. Pharm. Bull, in press.
22. Li, G., Xu, M. L., Lee, C. S., Woo, M. H., Chang, H. W., Son, J. K.: Cytotoxic and DNA topoisomerase inhibitory activity of constituents from the Sclerotium of Poria cocos. Arch. Pharm. Res., 27, 829-833, 2004.