Screening for enterotoxigenic Bacteroides fragilis in stool samples

Anaerobe

Volumn 40, Issue , 2016, Pages 50-53

  Keenan, Jacqueline I ^a   Aitchison, Alan ^a   Purcell, Rachel V ^a   Greenlees, Rosie ^b   Pearson, John F ^a   Frizelle, Frank A ^a  

^a UNIVERSITY OF OTAGO   (New Zealand)

^b CANTERBURY HEALTH LABORATORIES   (New Zealand)

Author keywords

bft gene; Colorectal cancer; Enterotoxigenic Bacteroides fragilis; PCR; Stool samples

Indexed keywords

BACTERIAL DNA; FRAGILYSIN; STERILE WATER; BACTERIAL TOXIN; METALLOPROTEINASE; PRIMER DNA;

ADULT; AGED; ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM COLONY; BACTERIUM CULTURE; BACTEROIDES FRAGILIS; BFT GENE; CARCINOGENESIS; COLORECTAL CANCER; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; FECES ANALYSIS; FEMALE; GENE AMPLIFICATION; HEAT TREATMENT; HUMAN; MAJOR CLINICAL STUDY; MALE; MASS SCREENING; MIDDLE AGED; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; BACTEROIDES INFECTIONS; BIOSYNTHESIS; CASE CONTROL STUDY; CHEMISTRY; COLORECTAL NEOPLASMS; EARLY CANCER DIAGNOSIS; FECES; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; PATHOGENICITY; PATHOLOGY; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STANDARDS; VERY ELDERLY; VIRULENCE;

AGED; AGED, 80 AND OVER; BACTERIAL TOXINS; BACTEROIDES FRAGILIS; BACTEROIDES INFECTIONS; CASE-CONTROL STUDIES; COLORECTAL NEOPLASMS; DNA PRIMERS; DNA, BACTERIAL; EARLY DETECTION OF CANCER; FECES; FEMALE; GENE EXPRESSION; GENES, BACTERIAL; HUMANS; MALE; METALLOENDOPEPTIDASES; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRULENCE;

EID: 84989900446     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2016.05.004     Document Type: Article

References (22)

1. Keenan, J.I., Frizelle, F.A., Bacteria flying under the radar linking a bacterial infection to colon carcinogenesis. Infect. Agent Cancer, 9, 2014, 31.
2. Mazmanian, S.K., Round, J.L., Kasper, D.L., A microbial symbiosis factor prevents intestinal inflammatory disease. Nature 453 (2008), 620–625.
3. Sears, C.L., Enterotoxigenic bacteroides fragilis: a rogue among symbiotes. Clin. Microbiol. Rev. 22 (2009), 349–369.
4. Toprak, N.U., Yagci, A., Gulluoglu, B.M., Akin, M.L., Demirkalem, P., Celenk, T., Soyletir, G., A possible role of bacteroides fragilis enterotoxin in the aetiology of colorectal cancer. Clin. Microbiol. Infect. 12 (2006), 782–786.
5. Viljoen, K.S., Dakshinamurthy, A., Goldberg, P., Blackburn, J.M., Quantitative profiling of colorectal cancer-associated bacteria reveals associations between fusobacterium spp., enterotoxigenic bacteroides fragilis (etbf) and clinicopathological features of colorectal cancer. PloS One, 10, 2015, e0119462.
6. Boleij, A., Hechenbleikner, E.M., Goodwin, A.C., Badani, R., Stein, E.M., Lazarev, M.G., Ellis, B., Carroll, K.C., Albesiano, E., Wick, E.C., Platz, E.A., Pardoll, D.M., Sears, C.L., The bacteroides fragilis toxin gene is prevalent in the colon mucosa of colorectal cancer patients. Clin. Infect. Dis. 60 (2015), 208–215.
7. Tjalsma, H., Boleij, A., Marchesi, J.R., Dutilh, B.E., A bacterial driver-passenger model for colorectal cancer: beyond the usual suspects. Nat. Rev. Microbiol. 10 (2012), 575–582.
8. Ferlay, J., Soerjomataram, I., Dikshit, R., Eser, S., Mathers, C., Rebelo, M., Parkin, D.M., Forman, D., Bray, F., Cancer incidence and mortality worldwide: sources, methods and major patterns in globocan 2012. Int. J. Cancer 136 (2015), E359–E386.
9. Pantosti, A., Malpeli, M., Wilks, M., Menozzi, M.G., D'Ambrosio, F., Detection of enterotoxigenic bacteroides fragilis by pcr. J. Clin. Microbiol. 35 (1997), 2482–2486.
10. Chen, L.A., Van Meerbeke, S., Albesiano, E., Goodwin, A., Wu, S., Yu, H., Carroll, K., Sears, C., Fecal detection of enterotoxigenic bacteroides fragilis. Eur. J. Clin. Microbiol. Infect. Dis. 34 (2015), 1871–1877.
11. Shetab, R., Cohen, S.H., Prindiville, T., Tang, Y.J., Cantrell, M., Rahmani, D., Silva, J. Jr., Detection of bacteroides fragilis enterotoxin gene by pcr. J. Clin. Microbiol. 36 (1998), 1729–1732.
12. Zhang, G., Weintraub, A., Rapid and sensitive assay for detection of enterotoxigenic bacteroides fragilis. J. Clin. Microbiol. 36 (1998), 3545–3548.
13. Livingston, S.J., Kominos, S.D., Yee, R.B., New medium for selection and presumptive identification of the bacteroides fragilis group. J. Clin. Microbiol. 7 (1978), 448–453.
14. Odamaki, T., Sugahara, H., Yonezawa, S., Yaeshima, T., Iwatsuki, K., Tanabe, S., Tominaga, T., Togashi, H., Benno, Y., Xiao, J.Z., Effect of the oral intake of yogurt containing bifidobacterium longum bb536 on the cell numbers of enterotoxigenic bacteroides fragilis in microbiota. Anaerobe 18 (2012), 14–18.
15. Aitchison, A., Frizelle, F.A., Keenan, J.I., PCR detection of bacteroides fragilis enterotoxin gene relies on robust primer design. J. Clin. Microbiol. 54 (2015), 239–240.
16. Dolezel, J., Bartos, J., Voglmayr, H., Greilhuber, J., Nuclear DNA content and genome size of trout and human. Cytom. Part A 51 (2003), 127–128 author reply 129.
17. Moncrief, J.S., Duncan, A.J., Wright, R.L., Barroso, L.A., Wilkins, T.D., Molecular characterization of the fragilysin pathogenicity islet of enterotoxigenic bacteroides fragilis. Infect. Immun. 66 (1998), 1735–1739.
18. Franco, A.A., Cheng, R.K., Goodman, A., Sears, C.L., Modulation of bft expression by the bacteroides fragilis pathogenicity island and its flanking region. Mol. Microbiol. 45 (2002), 1067–1077.
19. Rhee, K.J., Wu, S., Wu, X., Huso, D.L., Karim, B., Franco, A.A., Rabizadeh, S., Golub, J.E., Mathews, L.E., Shin, J., Sartor, R.B., Golenbock, D., Hamad, A.R., Gan, C.M., Housseau, F., Sears, C.L., Induction of persistent colitis by a human commensal, enterotoxigenic bacteroides fragilis, in wild-type c57bl/6 mice. Infect. Immun. 77 (2009), 1708–1718.
20. Sears, C.L., Garrett, W.S., Microbes, microbiota, and colon cancer. Cell Host Microbe 15 (2014), 317–328.
21. Salyers, A.A., Bacteroides of the human lower intestinal tract. Ann. Rev. Microbiol. 38 (1984), 293–313.
22. Moore, W.E., Holdeman, L.V., Human fecal flora: the normal flora of 20 japanese-hawaiians. Appl. Microbiol. 27 (1974), 961–979.