Detection of grape phylloxera (Daktulosphaira vitifoliae Fitch) by real-time quantitative PCR: development of a soil sampling protocol

Australian Journal of Grape and Wine Research

Volumn 22, Issue 3, 2016, Pages 469-477

  Giblot Ducray, D ^a   Correll, R ^b   Collins, C ^c   Nankivell, A ^d   Downs, A ^d   Pearce, I ^d   Mckay, A C ^a   Ophel Keller, K M ^a  

^a SOUTH AUSTRALIAN RESEARCH AND DEVELOPMENT INSTITUTE   (Australia)

^b Rho Environmetrics   (Australia)

^c UNIVERSITY OF ADELAIDE   (Australia)

^d National Wine Centre   (Australia)

Author keywords

composite sample; early detection; high throughput; phylloxera; surveillance

Indexed keywords

EID: 84988878514     PISSN: 13227130     EISSN: 17550238     Source Type: Journal    
DOI: 10.1111/ajgw.12237     Document Type: Article

References (16)

1. Benheim, D., Rochfort, S., Robertson, E., Potter, I.D. and Powell, K.S. (2012) Grape phylloxera (Daktulosphaira vitifoliae)—a review of potential detection and alternative management options. Annals of Applied Biology 161, 91–115.
2. Bruce, R.J., Powell, K.S., Lamb, D.W., Hoffmann, A.A. and Runting, J. (2011) Towards improved early detection of grapevine phylloxera (Daktulosphaira vitifoliae Fitch) using a risk-based assessment. Acta Horticulturae 904, 123–131.
3. Corrie, A.M., Crozier, R.H., Van Heeswijck, R. and Hoffmann, A.A. (2002) Clonal reproduction and population genetic structure of grape phylloxera, Daktulosphaira vitifoliae, in Australia. Heredity 88, 203–211.
4. Haling, E., Simpson, R.J., McKay, A.C., Hartley, D., Lambers, H., Ophel-Keller, K., Wiebkin, S., Herdina, Riley, I.T. and Richardson, A.E. (2011) Direct measurement of roots in soil for single and mixed species using a quantitative DNA-based method. Plant and Soil 348, 123–137.
5. Heap, J.W. and McKay, A.C. (2004) Spatial distribution of soilborne disease inoculum DNA in cereal crops and implications for soil sampling protocols. Ophel-Keller, K. and Hall, B., eds. Proceedings of the Third Australasian Soilborne Diseases Symposium; 8-11 February 2004; Rowland Flat, SA, Australia (South Australian Research and Development Institute: Adelaide, SA, Australia) pp. 106.
6. Herbert, K.S., Hoffmann, A.A. and Powell, K.S. (2006) Changes in grape phylloxera abundance in ungrafted vineyards. Journal of Economic Entomology 99, 1774–1783.
7. Herbert, K.S., Powell, K.S., McKay, A., Hartley, D., Herdina, Ophel-Keller, K., Schiffer, M. and Hoffmann, A. (2008) Developing and testing a diagnostic probe for grape phylloxera applicable to soil samples. Journal of Economic Entomology 101, 1934–1943.
8. Herdina, Neate, S., Jabaji-Hare, S. and Ophel-Keller, K. (2004a) Persistence of DNA of Gaeumannomyces graminis var. tritici in soil as measured by a DNA based assay. FEMS Microbiology Ecology 47, 143–152.
9. Herdina, Ophel-Keller, K., Wiebkin, S., Dumitrescu, I., Burns, R. and McKay, A. (2004b) The detection of dead soil-borne pathogens measured by a DNA based assay. Ophel-Keller, K. and Hall, B., eds. Proceedings of the Third Australasian Soilborne Diseases Symposium; 8–11 February 2004; Rowland Flat, SA, Australia (South Australian Research and Development Institute: Adelaide, SA, Australia) pp. 103–104.
10. Hollaway, G.J., Ophel-Keller, K.M., Taylor, S.P., Burns, R.A. and McKay, A.C. (2003) Effect of soil water content, sampling method and sample storage on the quantification of root lesion nematodes (Pratylenchus spp.) by different methods. Australasian Plant Pathology 32, 73–79.
11. National Vine Health Steering Committee (2009) National phylloxera management protocol. http://www.vinehealth.com.au/media/National-Phylloxera-Management-Protocol.pdf. Accessed July 14, 2015.
12. Ophel-Keller, K., McKay, A., Hartley, D., Herdina and Curran, J. (2008) Development of a routine DNA-based testing service for soilborne diseases in Australia. Australasian Plant Pathology 37, 243–253.
13. Powell, K.S. (2012a) A holistic approach to future management of grapevine phylloxera. Bostanian, N.J., Vincent, C. and Isaacs, R., eds. Arthropod Management in Vineyards: Pests, Approaches, and Future Directions (Springer: Berlin, Germany) pp. 219–251.
14. Powell, K.S. (2012b) The three R's – rootstock, resistance and resilience to grape phylloxera. Final report to Grape and Wine Research and Development Corporation. Project DPI 08/01 (Grape and Wine Research Development Corporation: Adelaide, SA, Australia) 157 pp. http://research.wineaustralia.com/completed_projects/the-three-rs-rootstock-resistance-and-resilience-to-grape-phylloxera/. Accessed July 14, 2015.
15. Powell, K.S., Slattery, W.F., Deretic, J., Herbert, K.S. and Hetherington, S. (2003) Influence of soil type and climate on the population dynamics and dispersal of grapevine phylloxera in Australia. Acta Horticulturae 617, 33–42.
16. R Core Team (2013) R: A language and environment for statistical computing. R Foundation for Statistical Computing: Vienna, Austria. http://www.R-project.org/. Accessed May 5, 2016.