Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures

Nature Protocols

Volumn 11, Issue 10, 2016, Pages 1955-1976

  Hiler, Daniel J ^a   Barabas, Marie E ^a   Griffiths, Lyra M ^a   Dyer, Michael A ^a,b,c  

^a ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

^b UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CLONE; CELL DIFFERENTIATION; CELL ISOLATION; CELL POPULATION; CELL PROLIFERATION; CLINICAL PROTOCOL; NERVE CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; RETINA NERVE CELL; TUMOR SUPPRESSOR GENE;

EID: 84988583074     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2016.109     Document Type: Article

References (18)

1. Schwartz, S. D. et al. Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: Follow-up of two open-label phase 1/2 studies. Lancet 385, 509-516 (2015).
2. Lamba, D. A., Gust, J. & Reh, T. A. Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice. Cell Stem Cell 4, 73-79 (2009).
3. MacLaren, R. E. et al. Retinal repair by transplantation of photoreceptor precursors. Nature 444, 203-207 (2006).
4. Pearson, R. A. et al. Restoration of vision after transplantation of photoreceptors. Nature 485, 99-103 (2012).
5. Eiraku, M. & Sasai, Y. Mouse embryonic stem cell culture for generation of three-dimensional retinal and cortical tissues. Nat. Protoc. 7, 69-79 (2012).
6. Eiraku, M. et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 472, 51-56 (2011).
7. Nakano, T. et al. Self-formation of optic cups and storable stratified neural retina from human ESCs. Cell Stem Cell 10, 771-785 (2012).
8. Hiler, D. et al. Quantification of retinogenesis in 3D cultures reveals epigenetic memory and higher efficiency in iPSCs derived from rod photoreceptors. Cell Stem Cell 17, 101-115 (2015).
9. Kim, K. et al. Epigenetic memory in induced pluripotent stem cells. Nature 467, 285-290 (2010).
10. Kim, J. et al. Reprogramming of postnatal neurons into induced pluripotent stem cells by defined factors. Stem Cells 29, 992-1000 (2011).
11. Stadtfeld, M., Maherali, N., Borkent, M. & Hochedlinger, K. A reprogrammable mouse strain from gene-targeted embryonic stem cells. Nat. Methods 7, 53-55 (2010).
12. Akimoto, M. et al. Targeting of GFP to newborn rods by Nrl promoter and temporal expression profiling of flow-sorted photoreceptors. Proc. Natl. Acad. Sci. USA 103, 3890-3895 (2006).
13. Lakowski, J. et al. Effective transplantation of photoreceptor precursor cells selected via cell surface antigen expression. Stem Cells 29, 1391-1404 (2011).
14. Institute of Laboratory Animal Resources. in Guide for the Care and Use of Laboratory Animals (National Research Council, 1996).
15. Conner, D. A Mouse embryo fibroblast (MEF) feeder cell preparation. Curr. Protoc. Mol. Biol. Chapter 23 Unit 23 22 (2001).
16. Pruszak, J., Sonntag, K. C., Aung, M. H., Sanchez-Pernaute, R. & Isacson, O. Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations. Stem Cells 25, 2257-2268 (2007).
17. Zhang, W. Y, de Almeida, P. E. & Wu, J. C. in StemBook http://www. ncbi. nlm. nih. gov/pubmed/?term=23658974 (2008).
18. Basu, S., Campbell, H. M., Dittel, B. N. & Ray, A. Purification of specific cell population by fluorescence activated cell sorting (FACS). J. Vis. Exp. http://dx. doi. org/10. 3791/1546 (2010).