πsPIM: High NA high resolution isotropic light-sheet imaging in cell culture dishes

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Theer, Patrick ^a,b   Dragneva, Denitsa ^a   Knop, Michael ^a,b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; ENDOCYTOSIS; FLUORESCENCE MICROSCOPY; HEK293 CELL LINE; HELA CELL LINE; HUMAN; PHYSIOLOGY; PROCEDURES; SACCHAROMYCES CEREVISIAE; THREE DIMENSIONAL IMAGING; TUMOR CELL LINE;

CELL LINE, TUMOR; ENDOCYTOSIS; HEK293 CELLS; HELA CELLS; HUMANS; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, FLUORESCENCE; SACCHAROMYCES CEREVISIAE;

EID: 84987725317     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep32880     Document Type: Article

References (24)

1. Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E. H. K. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305, 1007-1009 (2004).
2. Siedentopf, H. & Zsigmondy, R. Über Sichtbarmachung und Groessenbestimmung ultramikroskopischer Teilchen, mit besonderer Anwendung auf Goldrubinglaesern. Annalen der Physik 10, 1-39 (1903).
3. Konopka, C. A. & Bednarek, S. Y. Variable-angle epifluorescence microscopy: a new way to look at protein dynamics in the plant cell cortex. Plant J. 53, 186-196 (2008).
4. Dunsby, C. Optically sectioned imaging by oblique plane microscopy. Opt. Express 16, 20306-20316 (2008).
5. Tokunaga, M., Imamoto, N. & Sakata-Sogawa, K. Highly inclined thin illumination enables clear single-molecule imaging in cells. Nat Methods 5, 159-161 (2008).
6. Gebhardt, J. C. M. et al. Single-molecule imaging of transcription factor binding to DNA in live mammalian cells. Nat Methods doi: 10.1038/nmeth.2411 (2013).
7. Galland, R. et al. 3D high-and super-resolution imaging using single-objective SPIM. Nat Methods doi: 10.1038/nmeth.3402 (2015).
8. Bouchard, M. B. et al. Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms. Nat Photonics 9, 113-119 (2015).
9. Gu, M. Springer Series in Optical Sciences-Advanced Optical Imaging Theory 75, Chapter 6 (Springer, 2000).
10. Debye, P. Das Verhalten von Lichtwellen in der Nähe eines Brennpunktes oder einer Brennlinie. Annalen der Physik 335, 755-776 (1909).
11. Capoulade, J., Wachsmuth, M., Hufnagel, L. & Knop, M. Quantitative fluorescence imaging of protein diffusion and interaction in living cells. Nat. Biotechnol. 29, 835-839 (2011).
12. Gao, L. et al. Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Cell 151, 1370-1385 (2012).
13. Chen, B.-C. et al. Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution. Science 346, 1257998 (2014).
14. Theer, P., Mongis, C. & Knop, M. PSFj: know your fluorescence microscope. Nat Methods 11, 981-982 (2014).
15. Brach, T., Specht, T. & Kaksonen, M. Reassessment of the role of plasma membrane domains in the regulation of vesicular traffic in yeast. J. Cell. Sci. 124, 328-337 (2011).
16. Kaksonen, M., Toret, C. P. & Drubin, D. G. A modular design for the clathrin-and actin-mediated endocytosis machinery. Cell 123, 305-320 (2005).
17. Boeke, D. et al. Quantification of cytosolic interactions identifies Ede1 oligomers as key organizers of endocytosis. Mol. Syst. Biol. 10, 756 (2014).
18. Sheff, M. A. & Thorn, K. S. Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast 21, 661-670 (2004).
19. Lorenz, H., Hailey, D. W. & Lippincott-Schwartz, J. Fluorescence protease protection of GFP chimeras to reveal protein topology and subcellular localization. Nat. Methods 3, 205-210 (2006).
20. Distel, M., Hocking, J. C., Volkmann, K. & Köster, R. W. The centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in vivo. J. Cell Biol. 191, 875-890 (2010).
21. Neumann, B. et al. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464, 721-727 (2010).
22. Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat Methods 9, 676-682 (2012).
23. Bahlmann, K., Jakobs, S. & Hell, S. W. 4Pi-confocal microscopy of live cells. Ultramicroscopy 87, 155-164 (2001).
24. Planchon, T. A. et al. Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Nat Methods 8, 417-423 (2011).