High-resolution melting and real-time PCR for quantification and detection of drug-resistant HBV mutants in a single amplicon

Antiviral Therapy

Volumn 17, Issue 2, 2012, Pages 291-303

  Hsiao, Chia Chieh ^a   Chang, Juliana ^a   Wu, Jeng You ^a   Liu, Wan Hsin ^a   Han, Shih Yun ^a   Chen, Pei Jer ^a   Yeh, Shiou Hwei ^a,b  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

ADEFOVIR; ENTECAVIR; LAMIVUDINE;

ACCURACY; AMPLICON; ANTIVIRAL RESISTANCE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA DETERMINATION; FEASIBILITY STUDY; HEPATITIS B; HEPATITIS B VIRUS; HIGH RESOLUTION MELTING ANALYSIS; HUMAN; HYBRIDIZATION; MOLECULAR PROBE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATIENT MONITORING; PILOT STUDY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS LOAD; VIRUS MUTANT; WILD TYPE;

EID: 84984585740     PISSN: 13596535     EISSN: None     Source Type: Journal    
DOI: 10.3851/IMP2022     Document Type: Article

References (28)

1. Lee WM. Hepatitis B virus infection. N Engl J Med 1997; 337:1733-1745. (Pubitemid 27527771)
2. Lavanchy D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat 2004; 11:97-107. (Pubitemid 38365759)
3. Dienstag JL. Hepatitis B virus infection. N Engl J Med 2008; 359:1486-1500.
4. Hoofnagle JH, Doo E, Liang TJ, Fleischer R, Lok AS. Management of hepatitis B: summary of a clinical research workshop. Hepatology 2007; 45:1056-1075. (Pubitemid 46646501)
5. Bartholomeusz A, Locarnini SA. Antiviral drug resistance: clinical consequences and molecular aspects. Semin Liver Dis 2006; 26:162-170. (Pubitemid 43668258)
6. Ghany M, Liang TJ. Drug targets and molecular mechanisms of drug resistance in chronic hepatitis B. Gastroenterology 2007; 132:1574-1585. (Pubitemid 46627588)
7. Liaw YF, Chien RN, Yeh CT. No benefit to continue lamivudine therapy after emergence of YMDD mutations. Antivir Ther 2004; 9:257-262.
8. Zoulim F, Locarnini S. Hepatitis B virus resistance to nucleos(t)ide analogues. Gastroenterology 2009; 137:1593-1608.e2.
9. European Association for the Study of the Liver. EASL clinical practice guidelines: management of chronic hepatitis B. J Hepatol 2009; 50:227-242.
10. Yeon JE. Technique for the early detection of drug-resistant HBV DNA during antiviral therapy. Intervirology 2008; 51 Suppl 1:7-10. (Pubitemid 351821836)
11. Montgomery JL, Sanford LN, Wittwer CT. High-resolution DNA melting analysis in clinical research and diagnostics. Expert Rev Mol Diagn 2010; 10:219-240.
12. Wittwer CT. High-resolution DNA melting analysis: advancements and limitations. Hum Mutat 2009; 30:857-859.
13. Vossen RH, Aten E, Roos A, den Dunnen JT. Highresolution melting analysis (HRMA): more than just sequence variant screening. Hum Mutat 2009; 30:860-866.
14. Erali M, Voelkerding K V, Wittwer CT. High resolution melting applications for clinical laboratory medicine. Exp Mol Pathol 2008; 85:50-58.
15. Reed GH, Kent JO, Wittwer CT. High-resolution DNA melting analysis for simple and efficient molecular diagnostics. Pharmacogenomics 2007; 8:597-608. (Pubitemid 46939585)
16. Steer PA, Kirkpatrick NC, O'Rourke D, Noormohammadi AH. Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region. J Clin Microbiol 2009; 47:311-321.
17. Tajiri-Utagawa E, Hara M, Takahashi K, Watanabe M, Wakita T. Development of a rapid high-throughput method for high-resolution melting analysis for routine detection and genotyping of noroviruses. J Clin Microbiol 2009; 47:435-440.
18. Lin JH, Tseng CP, Chen YJ, et al. Rapid differentiation of influenza A virus subtypes and genetic screening for virus variants by high-resolution melting analysis. J Clin Microbiol 2008; 46:1090-1097.
19. Toi CS, Dwyer DE. Differentiation between vaccine and wild-type varicella-zoster virus genotypes by high-resolution melt analysis of single nucleotide polymorphisms. J Clin Virol 2008; 43:18-24.
20. Dames S, Pattison DC, Bromley LK, Wittwer CT, Voelkerding K V. Unlabeled probes for the detection and typing of herpes simplex virus. Clin Chem 2007; 53:1847-1854. (Pubitemid 47509680)
21. Carman W, Thomas H, Domingo E. Viral genetic variation: hepatitis B virus as a clinical example. Lancet 1993; 341:349-353. (Pubitemid 23039709)
22. Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT. Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clin Chem 2004; 50:1328-1335. (Pubitemid 38970639)
23. Margraf RL, Mao R, Wittwer CT. Masking selected sequence variation by incorporating mismatches into melting analysis probes. Hum Mutat 2006; 27:269-278.
24. Shih YH, Yeh SH, Chen PJ, et al. Hepatitis B virus quantification and detection of YMDD mutants in a single reaction by real-time PCR and annealing curve analysis. Antivir Ther 2008; 13:469-480.
25. Yeh SH, Tsai CY, Kao JH, et al. Quantification and genotyping of hepatitis B virus in a single reaction by realtime PCR and melting curve analysis. J Hepatol 2004; 41:659-666.
26. Zhou L, Wang L, Palais R, Pryor R, Wittwer CT. Highresolution DNA melting analysis for simultaneous mutation scanning and genotyping in solution. Clin Chem 2005; 51:1770-1777. (Pubitemid 41368166)
27. Liaw Y F. Antiviral therapy of chronic hepatitis B: opportunities and challenges in Asia. J Hepatol 2009; 51:403-410.
28. Dames S, Margraf RL, Pattison DC, Wittwer CT, Voelkerding KV. Characterization of aberrant melting peaks in unlabeled probe assays. J Mol Diagn 2007; 9:290-296. (Pubitemid 47305064)