Silencing of Mythimna separata chitinase genes via oral delivery of in planta-expressed RNAi effectors from a recombinant plant virus

Biotechnology Letters

Volumn 38, Issue 11, 2016, Pages 1961-1966

  Bao, Wenhua ^a   Cao, Budao ^a   Zhang, Yanan ^a   Wuriyanghan, Hada ^a  

^a INNER MONGOLIA UNIVERSITY   (China)

Author keywords

Chitinase; Mythimna separate; Nicotiana benthamiana; Nody weight; RNA interference; Virus vector

Indexed keywords

CLONING; GENE EXPRESSION; NUCLEIC ACIDS; PLANTS (BOTANY); RNA;

CHITINASES; MYTHIMNA SEPARATE; NICOTIANA BENTHAMIANA; NODY WEIGHT; RNA INTERFERENCE;

VIRUSES;

CHITINASE; INSECT PROTEIN;

ADMINISTRATION AND DOSAGE; ANIMAL; BODY WEIGHT; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENE VECTOR; GENETICS; INSECT CONTROL; LEPIDOPTERA; METABOLISM; ORAL DRUG ADMINISTRATION; PARASITOLOGY; PLANT VIRUS; RNA INTERFERENCE; TOBACCO; VIROLOGY;

ADMINISTRATION, ORAL; ANIMALS; BODY WEIGHT; CHITINASES; GENE EXPRESSION REGULATION; GENETIC VECTORS; INSECT CONTROL; INSECT PROTEINS; LEPIDOPTERA; PLANT VIRUSES; RNA INTERFERENCE; TOBACCO;

EID: 84982791343     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-016-2186-0     Document Type: Article

References (14)

1. Kamthan A, Chaudhuri A, Kamthan M, Datta A (2015) Small RNAs in plants: recent development and application for crop improvement. Front Plant Sci 6:208
2. Khajuria C, Buschman LL, Chen MS, Muthukrishnan S, Zhu KY (2010) A gut-specific chitinase gene essential for regulation of chitin content of peritrophic matrix and growth of Ostrinia nubilalis larvae. Insect Biochem Molec Biol 40:621–629
3. Khan AM, Ashfaq M, Khan AA, Rasool A, Iqbal J, Mansoor S (2015) Inoculation of Nicotiana tabacum with recombinant potato virus X induces RNA interference in the solenopsis mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: pseudococcidae). Biotechnol Lett 10:2083–2090
4. Kola VS, Renuka P, Madhav MS, Mangrauthia SK (2015) Key enzymes and proteins of crop insects as candidate for RNAi based gene silencing. Front Physiol 6:119
5. Lindbo JA (2007) High-efficiency protein expression in plants from agroinfection-compatible tobacco mosaic virus expression vectors. BMC Biotechnol 7:1–11
6. Matthias L, Yellina AL, Svetlana O, Annette B (2013) Virus-induced gene silencing (VIGS) in plants: an overview of target species and the virus-derived vector systems. Methods Mol Biol 975:1–14
7. McCoy CJ, Warnock ND, Atkinson LE, Atcheson E, Martin RJ, Robertson AP, Maule AG, Marks NJ, Mousley A (2015) RNA interference in adult Ascaris suum-an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite. Int J Parasitol 45:673–678
8. Nagata Y, Lee JM, Mon H, Imanishi S, Hong SM, Komatsu S, Oshima Y, Kusakabe T (2013) RNAi suppression of β-N-acetylglucosaminidase (BmFDL) for complex-type N-linked glycan synthesis in cultured silkworm cells. Biotechnol Lett 35:1009–1016
9. Pavan K, Subhash PS, Baldwin IT (2012) Tobacco rattle virus vector: a rapid and transient means of silencing Manduca sexta genes by plant mediated RNA interference. PLoS ONE 7:277–291
10. Wuriyanghan H, Falk BW (2013) RNA interference towards the potato psyllid, Bactericera cockerelli, is induced in plants infected with recombinant tobacco mosaic virus (TMV). PLoS ONE 8:e66050
11. Yasuyuki A, Specht CA, Kramer KJ, Subbaratnam M, Beeman RW (2008) Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum. Insect Biochem Molec Biol 38:959–962
12. Zhang J, Liu X, Zhang J, Li D, Sun Y, Guo Y, Ma E, Zhu KY (2010a) Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (meyen). Insect Biochem Molec Biol 40:824–833
13. Zhang X, Zhang J, Zhu KY (2010b) Chitosan/double-stranded RNA nanoparticle-mediated RNA interference to silence chitin synthase genes through larval feeding in the african malaria mosquito (Anopheles gambiae). Insect Molec Biol 19:683–693
14. Zhi X, Xiang Y, Yang C, Ding G, Chen H, Xu H (2014) Synthesis of 4β-acyloxypodophyllotoxin analogs modified in the c and e rings as insecticidal agents against Mythimna separata walker. Bioorg Med Chem Lett 24:765–772