Detection of the plasmid-mediated colistin-resistance gene mcr-1 in clinical isolates and stool specimens obtained from hospitalized patients using a newly developed real-time PCR assay

Journal of Antimicrobial Chemotherapy

Volumn 71, Issue 8, 2016, Pages 2344-2346

  Nijhuis, R H T ^a   Veldman, K T ^b   Schelfaut, J ^a   Van Essen Zandbergen, A ^b   Wessels, E ^a   Claas, E C J ^a   Gooskens, J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LYSATE; COLISTIN; DNA; ESCHERICHIA COLI PROTEIN; MCR-1 PROTEIN, E COLI;

ACINETOBACTER; AEROMONAS; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM ISOLATE; BROTH DILUTION; CENTRIFUGATION; COLONY FORMING UNIT; DNA EXTRACTION; DNA ISOLATION; ENTEROBACTER; ESCHERICHIA COLI; FECES ANALYSIS; GENE AMPLIFICATION; HOSPITAL PATIENT; HUMAN; KLEBSIELLA; LETTER; LIMIT OF DETECTION; MCR 1 GENE; NONHUMAN; PSEUDOMONAS; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA; SEQUENCE ANALYSIS; SUPERNATANT; SUSPENSION; VIBRIO; ANTIBIOTIC RESISTANCE; ESCHERICHIA COLI INFECTION; FECES; GENETICS; HOSPITALIZATION; ISOLATION AND PURIFICATION; MICROBIOLOGY; MULTILOCUS SEQUENCE TYPING; NETHERLANDS; PLASMID; PROCEDURES; TERTIARY CARE CENTER;

COLISTIN; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; ESCHERICHIA COLI PROTEINS; FECES; GENES, BACTERIAL; HOSPITALIZATION; HUMANS; MULTILOCUS SEQUENCE TYPING; NETHERLANDS; PLASMIDS; REAL-TIME POLYMERASE CHAIN REACTION; TERTIARY CARE CENTERS;

EID: 84982233919     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkw192     Document Type: Letter

References (10)

1. Liu YY, Wang Y, Walsh TR et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis 2016; 16: 161-8.
2. Arcilla MS, van Hattem JM, Matamoros S et al. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 2016; 16: 147-9.
3. Falgenhauer L, Waezsada SE, Yao Y et al. Colistin resistance gene mcr-1 in extended-spectrum β-lactamase-producing and carbapenemaseproducing Gram-negative bacteria in Germany. Lancet Infect Dis 2016; 16: 282-3.
4. Hasman H, Hammerum AM, Hansen F et al. Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015. Euro Surveill 2015; 20: pii=30085.
5. Poirel L, Kieffer N, Liassine N et al. Plasmid-mediated carbapenem and colistin resistance in a clinical isolate of Escherichia coli. Lancet Infect Dis 2016; 16: 281.
6. Stoesser N, Mathers AJ, Moore CE et al. Colistin resistance gene mcr-1 and pHNSHP45 plasmid in human isolates of Escherichia coli and Klebsiella pneumoniae. Lancet Infect Dis 2016; 16: 285-6.
7. Zeng KJ, Doi Y, Patil S et al. Emergence of the plasmid-mediated mcr-1 gene in colistin-resistant Enterobacter aerogenes and Enterobacter cloacae. Antimicrob Agents Chemother 2016; 60: 3862-3.
8. van Doornum GJ, Guldemeester J, Osterhaus AD et al. Diagnosing herpesvirus infections by real-time amplification and rapid culture. J Clin Microbiol 2003; 41: 576-80.
9. Tse H, Yuen KY. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 2016; 16: 145-6.
10. Webb HE, Granier SA, Marault M et al. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis 2016; 16: 144-5.