Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons

Science

Volumn 353, Issue 6302, 2016, Pages 925-928

  Habib, Naomi ^a   Li, Yinqing ^a,b,c   Heidenreich, Matthias ^a,b,c   Swiech, Lukasz ^a,b,c   Avraham Davidi, Inbal ^a   Trombetta, John J ^a   Hession, Cynthia ^a   Zhang, Feng ^a,b,d,e,f   Regev, Aviv ^a,e  

^a BROAD INSTITUTE OF MIT AND HARVARD   (United States)

^b Stanley Center for Psychiatric Research   (United States)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d USA   (United States)

^e MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^f Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; EDOXUDINE; RNA; TRANSCRIPTION FACTOR MEIS2; TRANSCRIPTION FACTOR PBX3; UNCLASSIFIED DRUG; 5-ETHYNYL-2'-DEOXYURIDINE; DEOXYURIDINE; TRANSCRIPTOME;

ADULT; CELLS AND CELL COMPONENTS; COMPLEXITY; NEUROLOGY; RNA; SENSITIVITY ANALYSIS;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; CELLS BY BODY ANATOMY; DIV SEQUENCING; DRAXIN GENE; FLUORESCENCE ACTIVATED CELL SORTING; GAD1 GENE; GAD2 GENE; GENE; GENE EXPRESSION; GENE IDENTIFICATION; HIPPOCAMPAL CA1 REGION; HIPPOCAMPAL CA2 REGION; HIPPOCAMPAL CA3 REGION; IN SITU HYBRIDIZATION; MOUSE; NERVE CELL; NERVOUS SYSTEM DEVELOPMENT; NEWBORN; NONHUMAN; OPRD1 GENE; PENK GENE; PRIORITY JOURNAL; RNA SEQUENCE; RRM2 GENE; SEQUENCE ANALYSIS; SINGLE NUCLEUS RNA SEQUENCING; SPINAL CORD; SPINAL GANGLION; ANALOGS AND DERIVATIVES; ANIMAL; CELL DIVISION; CELL NUCLEUS; CYTOLOGY; GENETIC TRANSCRIPTION; GENETICS; HIPPOCAMPUS; ISOTOPE LABELING; METABOLISM; PATHOLOGY; PROCEDURES; SINGLE CELL ANALYSIS;

ANIMALS; CELL DIVISION; CELL NUCLEUS; DEOXYURIDINE; HIPPOCAMPUS; ISOTOPE LABELING; MICE; NEUROGENESIS; NEURONS; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; SPINAL CORD; TRANSCRIPTION, GENETIC; TRANSCRIPTOME;

EID: 84980325679     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aad7038     Document Type: Article

References (21)

1. A. Zeisel et al., Science 347, 1138-1142 (2015).
2. J. Shin et al., Cell Stem Cell 17, 360-372 (2015).
3. B. Tasic et al., Nat. Neurosci. 19, 335-346 (2016).
4. G. L. Ming, H. Song, Neuron 70, 687-702 (2011).
5. D. L. Moore, G. A. Pilz, M. J. Araúzo-Bravo, Y. Barral, S. Jessberger, Science 349, 1334-1338 (2015).
6. Materials and methods are available as supplementary materials on Science Online.
7. B. Lacar et al., Nat. Commun. 7, 11022 (2016).
8. L. Swiech et al., Nat. Biotechnol. 33, 102-106 (2015).
9. H. Hu, J. Gan, P. Jonas, Science 345, 1255263 (2014).
10. E. S. Lein et al, Nature 445, 168-176 (2007).
11. Y. Zhang et al., J. Neurosci. 34, 11929-11947 (2014).
12. M. S. Cembrowski et al, Neuron 89, 351-368 (2016).
13. B. P. Roques, M. C. Fournié-Zaluski, M. Wurm, Nat. Rev. Drug Discov. 11, 292-310 (2012).
14. E. Llorens-Bobadilla et al., Cell Stem Cell 17, 329-340 (2015).
15. M. Schouten, M. R. Buijink, P. J. Lucassen, C. P. Fitzsimons, Front. Neurosci. 6, 25 (2012).
16. D. M. Feliciano, A. Bordey, L. Bonfanti, Cold Spring Harb. Perspect. Biol. 7, a018846 (2015).
17. P. J. Horner et al, J. Neurosci. 20, 2218-2228 (2000).
18. R. Shechter, Y. Ziv, M. Schwartz, Stem Cells 25, 2277-2282 (2007).
19. Z. Agoston et al., Development 141, 28-38 (2014).
20. C. A. Rottkamp, K. J. Lobur, C. L. Wladyka, A. K. Lucky, S. O'Gorman, Dev. Biol. 314, 23-39 (2008).
21. S. Picelli et al., Nat. Methods 10, 1096-1098 (2013).