Plant regeneration from protoplasts of alfalfa (Medicago sativa) via somatic embryogenesis;Regeneração de plantas a partir de protoplastos de alfafa (medicago sativa) via embriogênese somática

Scientia Agricola

Volumn 60, Issue 4, 2003, Pages 683-689

  Monteiro, Mariza ^a   Appezzato Da glória, Beatriz ^a   Valarini, Maria José ^b   de Oliveira, Carlos Alberto ^a   Vieira, Maria Lucia Carneiro ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b INSTITUTO DE ZOOTECNIA   (Brazil)

Author keywords

Forage legume; In vitro culture; Morphogenesis; Plant biotechnology

Indexed keywords

EID: 84978648110     PISSN: 01039016     EISSN: 1678992X     Source Type: Journal    
DOI: 10.1590/S0103-90162003000400012     Document Type: Article

References (39)

1. ARAGÃO, F.J.L.; BARROS, L.M.G.; DE SOUZA, M.V.; GROSSI DE SÁ, M.F.; ALMEIDA, E.R.P.; GANDER, E.S.; RECH, E.L. Expression of a methionine-rich storage albumin from the Brazil nut (Bertholletia excelsa H.B.K., Lecythidaceae) in transgenic bean plants (Phaseolus vulgaris L., Fabaceae). Genetics and Molecular Biology, v.22, p.445-449, 1999.
2. ATANASSOV, A.; BROWN, D.C.W. Plant regeneration from suspension culture and mesophyll protoplasts of Medicago sativa L. Plant Cell, Tissue and Organ Culture, v.3, p.149-162, 1984.
3. BINGHAM, E.T.; HURLEY, L.V.; KAATZ, D.M.; SAUNDERS, J.W. Breeding alfalfa which regenerates from callus tissue in culture. Crop Science, v.15, p.719-721, 1975.
4. CHEN, T.H.H.; MAROWITCH, J.; THOMPSON, B.G. Genotypic effects on somatic embryogenesis and plant regeneration from callus cultures of alfalfa. Plant Cell, Tissue and Organ Culture, v.8, p.73-81, 1987.
5. COELHO, W Jr.. Produção de sementes de alfafa: aspectos e considerações sobre comercialização. In: WORKSHOP SOBRE O POTENCIAL FORRAGEIRO DA ALFAFA (Medicago sativa L.) NOS TRÓPICOS. Juíz de Fora, 1994. Anais, Juíz de Fora: EMBRAPA-CNPGL; 1994, p.81-90.
6. D’HALLUIN, K.; BOTTERMAN, J.; DE GREEF, W. Engineering of herbicide-resistant alfalfa and evaluation under field conditions. Crop Science, v.30, p.866-871, 1990.
7. DICE, L.R. Measures of the amount of ecological association between species. Ecology, v.26, p.297-302, 1945.
8. DORNELAS, M.C.; VIEIRA, M.L.C. Plant regeneration from protoplasts cultures of Passiflora edulis var flavicarpa Deg., P. amethystina Mikan and P. cincinnata Mast. Plant Cell Reports, v.13, p.103-106, 1993.
9. DORNELAS, M.C.; VIEIRA, M.L.C.; APPEZZATO-DA-GLORIA, B. Histological analysis of organogenesis and somatic embryogenesis induced in immature tissues of Stylosanthes scabra Vog. Annals of Botany, v.70, p.477-482, 1992.
10. DU, S.; ERICKSON, L.; BOWLEY, S. Effect of plant genotype on the transformation of cultivated alfalfa (Medicago sativa) by Agrobacterium tumefaciens. Plant Cell Reports, v.13, p.330-334, 1994.
11. FERNANDO, J.A.; VIEIRA, M.L.C.; GERALDI, I.O.; APPEZZATO-DA-GLÓRIA, B. Anatomy of somatic embryogenesis in Glycine max (L.) Merrill. Brazilian Archives of Biology and Technology, v.45, p. 277-286, 2002.
12. FREARSON, E.M.; POWER, J.B.; COCKING E.C. The isolation, culture and regeneration of Petunia leaf protoplasts. Developmental Biology, v.33, p.130-137, 1973.
13. GAMBORG, O.L.; MILLER, R.A.; OJIMA, K. Nutrient requirements of suspension culture of soybean roots cells. Experimental Cell Research, v.50, p.151-158, 1968.
14. GESTEIRA, A.S.; OTONI, W.C.; BARROS, E.G.; MOREIRA, M.A. RAPD-based detection of genomic instability in soybean plants derived from somatic embryogenesis. Plant Breeding, v.121, p.269-271, 2002.
15. GILMOUR, D.M.; DAVEY, M.R.; COCKING, E.C. Production of somatic hybrid tissues following chemical and eletrical fusion of protoplasts from albino cell suspensions of Medicago sativa and M. borealis. Plant Cell Reports. v.8, p.29-32, 1989.
16. GROSSER, J.W.; COLLINS, G.B. Isolation and culture of Trifolium rubens protoplasts with whole plant regeneration, Plant Science Letters, v.37, p165-170, 1984.
17. HADDAD, C.M.; CASTRO, F.G.F. Sistemas de produção. In: SIMPÓSIO SOBRE MANEJO DA PASTAGEM: ALFAFA. 16., Piracicaba, 1999. Anais. Piracicaba: FEALQ, 1999, p.7-22.
18. KAO, K.N. Chromosomal behaviour in somatic hybrids of Soybean-Nicotiana glauca. Molecular and General Genetics, v.150, p.225-230, 1977.
19. KAO, K.N.; MICHAYLUK, M.R. Plant regeneration from mesophyll protoplasts of alfalfa. Zeitschrift für Pflanzenphysiology, v.96, p.135-141, 1980.
20. KARNOVSKY, M.J. A formaldehyde-glutaraldehyde fixative of high osmolarity for use electron microscopy. Journal of Cell Biology, v.27, p.137-138, 1965.
21. McKERSEI, B. D.; CHEN, Y.; DE BEUS, M.; BOWLEY, S. R.; BOWLER, C., INZÉ, D.; D’HALUIN, K.; BOTTERMAN, J. Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.). Plant Physiology, v. 103, p. 1155-1163, 1993.
22. MENDIS, M.H.; POWER, J.B.; DAVEY, M.R. Somatic hybrids of the forage legumes Medicago sativa L. and M. falcata L. Journal of Experimental Botany, v.42, p.1565-1573, 1991.
23. MONTEIRO, M. Regeneração de plantas a partir de protoplastos de alfafa (Medicago sativa L.) e otimização da técnica de fusão química entre M. sativa L. e M. arborea L. Piracicaba: USP/ESALQ, 2000. 172p. (Dissertação - Mestrado)
24. MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.
25. MYERS, J.R.; GROSSER, J.W.; TAYLOR, N.L.; COLLINS, G.B. Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.). Plant Cell, Tissue and Organ Culture. V.19, p.113-127, 1989.
26. NENZ, E.; PUPILLI, F.; DAMIANI, F.; ARCIONI, S. Somatic hybrid plants between the forage legumes Medicago sativa L. and Medicago arborea L. Theoretical and Applied Genetics, v.93, p.183-189, 1996.
27. PARROT, W.A.; DURHAM, R.E.; BAYLEY, M.A. Somatic embryogenesis in legumes. In: BAJAJ, Y.P.S. (Ed.) Biotechnology in agriculture and forestry. New York: Springer-Verlag, 1995. v.29, p.199-222.
28. PICCIONI, E.; BARCACCIA, G.; FALCINELLI, M.; STANDARDI, A. Estimating alfalfa somaclonal variation in axillary branching propagation and indirect somatic embryogenesis by RAPD fingerprinting. Journal of Plant Science, v.158, p.556-562, 1997.
29. POTRYKUS, I. Gene transfer to plants: assessment of published approaches and results. Annual Review of Plant Physiology and Plant Molecular Biology, v.42, p.205-225, 1991.
30. PUPILLI, F.; SCARPA, G.M.; CAMIANI, F.; ARCIONI, S. Interspecific somatic hybrids plants in the genus Medicago develop through protoplast fusion. Theoretical and Applied Genetics. v.84, p.792-797, 1992.
31. QUECINI, V.M. Transferência direta de genes para plantas de Stylosanthes guianensis (Aubl.) Sw. Piracicaba: USP/ESALQ, 1999. 172p. (Tese -Doutorado)
32. QUECINI, V.M.; OLIVEIRA, C.A.; ALVES, A.C.; VIEIRA, M.L.C. Factors influencing electroporation-mediated gene transfer to Stylosanthes guianensis (Aubl.) Sw. protoplasts. Genetics and Molecular Biologyy, v.25, p.73-80, 2002.
33. SAKAI, W.S. Simple method for different staining of parafin embedded plant material using toluidine blue O. Stain Technology, v.48, p.247-249, 1973.
34. SCHROEDER, H.E.; KHAN, M.R.I.; KNIBB, W.R.; SPENCER, D.; HIGGIS, T.J.V. Expression of a chicken ovalbumin gene en three lucerne cultivars. Australian Journal of Plant Physiology, v.18, p.495-505, 1991.
35. SHENK, B.U.; HILDEBRANDT, A.C. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany, v.50, p.199-204, 1972.
36. TÉOULÉ, E.; DATTÉE, Y. Recherche d’une méthode fiable de culture de protoplastes, d’hybridation somatique et de régénération chez Medicago. Agronomie, v.7, p.575-584, 1987.
37. UCHIMIYA, H.; MURASHIGE, T. Evaluation of parameters in isolation of viable protoplasts from cultured tobacco cells. Plant Physiology, v.54, p.944-963, 1974.
38. VIEIRA, M.L.C.; FUNGARO, M.H.P.; JUBIER, M.F.; LEJEUNE, B. Determination of taxonomic relationships among Brazilian taxa of Stylosanthes Sw., Leguminosae, using RAPD markers. Pesquisa Agropecuária Brasileira, v.3, p.137-142, 1997.
39. WALKER, K.A.; WENDELN, M.L.; JAWORSKI, E.G. Organogenesis in callus tissue of Medicago sativa. The temporal separation of induction processes from diferentiation processes. Plant Science Letters, v.16, p.23-30, 1979.