Microfluidics-based side view flow chamber reveals tether-to-sling transition in rolling neutrophils

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Marki, Alex ^a   Gutierrez, Edgar ^b   Mikulski, Zbigniew ^a   Groisman, Alex ^b   Ley, Klaus ^a,c  

^a LA JOLLA INSTITUTE FOR ALLERGY AND IMMUNOLOGY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BAYSILON; DIMETICONE; PADGEM PROTEIN;

ANIMAL; BONE MARROW CELL; CELL MOTION; CHEMISTRY; CONFOCAL MICROSCOPY; CYTOLOGY; DEVICES; LEUKOCYTE ROLLING; METABOLISM; MICROFLUIDICS; MOUSE; NEUTROPHIL; PHYSIOLOGY; PROCEDURES;

ANIMALS; BONE MARROW CELLS; CELL MOVEMENT; DIMETHYLPOLYSILOXANES; LEUKOCYTE ROLLING; MICE; MICROFLUIDICS; MICROSCOPY, CONFOCAL; NEUTROPHILS; P-SELECTIN;

EID: 84977126549     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep28870     Document Type: Article

References (36)

1. Kolaczkowska, E. and Kubes, P. Neutrophil recruitment and function in health and inflammation. Nature Reviews. Immunology 13, 159-175 (2013).
2. Beyrau, M., Bodkin, J. V. and Nourshargh, S. Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity. Open Biology 2, 120134 (2012).
3. Mocsai, A. Diverse novel functions of neutrophils in immunity, inflammation, and beyond. The Journal of Experimental Medicine 210, 1283-1299 (2013).
4. McEver, R. P. Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovascular Research 107, 331-339 (2015).
5. Schmidt, S., Moser, M. and Sperandio, M. The molecular basis of leukocyte recruitment and its deficiencies. Molecular Immunology 55, 49-58 (2013).
6. Sundd, P. et al. Slings enable neutrophil rolling at high shear. Nature 488, 399-403 (2012).
7. Pospieszalska, M. K., Lasiecka, I. and Ley, K. Cell protrusions and tethers: a unified approach. Biophysical Journal 100, 1697-1707 (2011).
8. Sundd, P., Pospieszalska, M. K. and Ley, K. Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings. Molecular Immunology 55, 59-69 (2013).
9. Schmidtke, D. W. and Diamond, S. L. Direct Observation of Membrane Tethers Formed during Neutrophil Attachment to Platelets or P-Selectin under Physiological Flow. The Journal of Cell Biology 149, 719-730 (2000).
10. Ramachandran, V., Williams, M., Yago, T., Schmidtke, D. W. and McEver, R. P. Dynamic alterations of membrane tethers stabilize leukocyte rolling on P-selectin. Proceedings of the National Academy of Sciences of the United States of America 101, 13519-13524 (2004).
11. Sundd, P. et al. Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling. Nature Methods 7, 821-824 (2010).
12. Rullo, J. et al. Actin polymerization stabilizes alpha4beta1 integrin anchors that mediate monocyte adhesion. J Cell Biol 197, 115-129 (2012).
13. Cao, J., Usami, S. and Dong, C. Development of a side-view chamber for studying cell-surface adhesion under flow conditions. Annals of Biomedical Engineering 25, 573-580 (1997).
14. Dong, C. and Lei, X. X. Biomechanics of cell rolling: shear flow, cell-surface adhesion, and cell deformability. Journal of Biomechanics 33, 35-43 (2000).
15. Gonnermann, C. et al. Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup. Integrative Biology: Quantitative Biosciences from Nano to Macro 7, 356-363 (2015).
16. Chaudhuri, O., Parekh, S. H., Lam, W. A. and Fletcher, D. A. Combined atomic force microscopy and side-view optical imaging for mechanical studies of cells. Nat Meth 6, 383-387 (2009).
17. Yuan, J., Melder, R. J., Jain, R. K. and Munn, L. L. Lateral view flow system for studies of cell adhesion and deformation under flow conditions. BioTechniques 30, 388-394 (2001).
18. Tkachenko, E., Gutierrez, E., Ginsberg, M. H. and Groisman, A. An easy to assemble microfluidic perfusion device with a magnetic clamp. Lab on a Chip 9, 1085-1095 (2009).
19. Damiano, E. R., Westheider, J., Tozeren, A. and Ley, K. Variation in the velocity, deformation, and adhesion energy density of leukocytes rolling within venules. Circulation Research 79, 1122-1130 (1996).
20. Firrell, J. C. and Lipowsky, H. H. Leukocyte margination and deformation in mesenteric venules of rat. American Journal of Physiology - Heart and Circulatory Physiology 256, H1667-H1674 (1989).
21. Pospieszalska, M. K., Zarbock, A., Pickard, J. E. and Ley, K. Event Tracking Model of Adhesion Identifies Load-bearing Bonds in Rolling Leukocytes. Microcirculation 16, 115-130 (2009).
22. Pospieszalska, M. K. and Ley, K. Dynamics of Microvillus Extension and Tether Formation in Rolling Leukocytes. Cellular and Molecular Bioengineering 2, 207-217 (2009).
23. Xu, G. and Shao, J.-Y. Human neutrophil surface protrusion under a point load: location independence and viscoelasticity. American Journal of Physiology - Cell Physiology 295, C1434-C1444 (2008).
24. Yu, Y. and Shao, J. Y. Simultaneous tether extraction contributes to neutrophil rolling stabilization: a model study. Biophysical Journal 92, 418-429 (2007).
25. Shao, J. Y., Ting-Beall, H. P. and Hochmuth, R. M. Static and dynamic lengths of neutrophil microvilli. Proceedings of the National Academy of Sciences of the United States of America 95, 6797-6802 (1998).
26. Waugh, R. E. Membrane Tethers. In Leukocyte Adhesion (ed. Ley, K.) 3-24 (Academic Press, Amsterdam, 2009).
27. Waugh, R. E. and Bausermann, R. G. Physical measurements of bilayer-skeletal separation forces. Ann. Biomed. Eng. 23, 308-321 (1995).
28. Waugh, R. E. and Hochmuth, R. M. Mechanical equilibrium of thick, hollow, liquid membrane cylinders. Biophys. J. 52, 391-400 (1987).
29. Pickard, J. E. and Ley, K. Micro-PTV measurement of the fluid shear stress acting on adherent leukocytes in vivo. Biophysical Journal 96, 4249-4259 (2009).
30. Liu, B. and Shao, J. Y. Tangential tether extraction and spontaneous tether retraction of human neutrophils. Biophysical Journal 103, 2257-2264 (2012).
31. Datar, A., Bornschlogl, T., Bassereau, P., Prost, J. and Pullarkat, P. A. Dynamics of membrane tethers reveal novel aspects of cytoskeleton-membrane interactions in axons. Biophysical Journal 108, 489-497 (2015).
32. Dopheide, S. M., Maxwell, M. J. and Jackson, S. P. Shear-dependent tether formation during platelet translocation on von Willebrand factor. Blood 99, 159-167 (2002).
33. Coghill, P. A., Kesselhuth, E. K., Shimp, E. A., Khismatullin, D. B. and Schmidtke, D. W. Effects of microfluidic channel geometry on leukocyte rolling assays. Biomedical Microdevices 15, 183-193 (2013).
34. Angiari, S. Selectin-mediated leukocyte trafficking during the development of autoimmune disease. Autoimmunity Reviews 14, 984-995 (2015).
35. Colace, T. V., Tormoen, G. W., McCarty, O. J. and Diamond, S. L. Microfluidics and coagulation biology. Annual Review of Biomedical Engineering 15, 283-303 (2013).
36. Combariza, M. E., Xinghuo, Y., Nesbitt, W. S., Mitchell, A. and Tovar-Lopez, F. J. Nonlinear Dynamic Modelling of Platelet Aggregation via Microfluidic Devices. Biomedical Engineering, IEEE Transactions on 62, 1718-1727 (2015).