Purification of HIV-1 gag virus-like particles and separation of other extracellular particles

Journal of Chromatography A

Volumn 1455, Issue , 2016, Pages 93-101

  Steppert, Petra ^a   Burgstaller, Daniel ^a   Klausberger, Miriam ^a   Berger, Eva ^b   Aguilar, Patricia Pereira ^a   Schneider, Tobias A ^b   Kramberger, Petra ^c   Tover, Andres ^d   Nöbauer, Katharina ^e   Razzazi Fazeli, Ebrahim ^e   Jungbauer, Alois ^a,b  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^c BIA SEPARATIONS D O O   (Slovenia)

^d Icosagen AS   (Estonia)

^e UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

Chromatography; Density gradient centrifugation; Extracellular particles; Host cell DNA; Host cell protein; Vaccines

Indexed keywords

CELL CULTURE; CELLS; CENTRIFUGATION; CHROMATOGRAPHY; DNA; PURIFICATION; VACCINES; VIRUSES;

CHROMATOGRAPHIC PROCEDURE; DENSITY GRADIENT CENTRIFUGATION; DOWNSTREAM-PROCESSING; EXTRACELLULAR; HOST CELL PROTEIN; HOST CELLS; PURIFICATION PROCESS; VIRUS-LIKE PARTICLES;

DISEASES;

BENZONASE; BIOLOGICAL MARKER; DNA; ENZYME; UNCLASSIFIED DRUG; GAG PROTEIN; NANOPARTICLE; RECOMBINANT PROTEIN; VIRUS LIKE PARTICLE VACCINE;

ANION EXCHANGE; ARTICLE; CELL CULTURE; CHO CELL LINE; CHROMATOGRAPHY; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; DIAGNOSTIC PROCEDURE; EXOSOME; EXTRACELLULAR PARTICLE; EXTRACELLULAR SPACE; HOST CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 GAG VIRUS LIKE PARTICLE; INTERMETHOD COMPARISON; MEMBRANE MICROPARTICLE; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PRODUCTIVITY; PROTEOMICS; SUPERNATANT; VIRUS PARTICLE; VIRUS PURIFICATION; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CRICETULUS; GENETICS; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; CENTRIFUGATION, DENSITY GRADIENT; CHO CELLS; CRICETINAE; CRICETULUS; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; HIV-1; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; NANOPARTICLES; RECOMBINANT PROTEINS; VACCINES, VIRUS-LIKE PARTICLE;

EID: 84973520076     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2016.05.053     Document Type: Article

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