A Droplet Microfluidic Platform for Automating Genetic Engineering

ACS Synthetic Biology

Volumn 5, Issue 5, 2016, Pages 426-433

  Gach, Philip C ^a,b   Shih, Steve C C ^a,b   Sustarich, Jess ^a,b   Keasling, Jay D ^a,c,d,e   Hillson, Nathan J ^a,c,f   Adams, Paul D ^a,c,d   Singh, Anup K ^a,b  

^a JOINT BIOENERGY INSTITUTE   (United States)

^b SANDIA NATIONAL LABORATORIES   (United States)

^c LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^d University of California   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f DOE JOINT GENOME INSTITUTE   (United States)

Author keywords

cell culture; digital microfluidics; molecular biology; transformation

Indexed keywords

DNA; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN;

ANTIBIOTIC RESISTANCE; ARTICLE; ASPERGILLUS NIGER; CULTURE MEDIUM; ESCHERICHIA COLI; FILAMENTOUS FUNGUS; GENETIC ENGINEERING; HEAT SHOCK; HOST; HYBRID; MICROFLUIDICS; MOLECULAR BIOLOGY; NONHUMAN; OXYGEN SUPPLY; PENICILLIN RESISTANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; TEMPERATURE; GENETICS; MICROFLUIDIC ANALYSIS; PLASMID; PROCEDURES;

DNA; ESCHERICHIA COLI; GENETIC ENGINEERING; MICROFLUIDIC ANALYTICAL TECHNIQUES; MICROFLUIDICS; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84973141722     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00011     Document Type: Article

References (29)

1. Cameron, D. E., Bashor, C. J., and Collins, J. J. (2014) A brief history of synthetic biology Nat. Rev. Microbiol. 12, 381-390 10.1038/nrmicro3239
2. Leguia, M., Brophy, J. A., Densmore, D., Asante, A., and Anderson, J. C. (2013) 2ab assembly: a methodology for automatable, high-throughput assembly of standard biological parts J. Biol. Eng. 7, 2 10.1186/1754-1611-7-2
3. Peralta-Yahya, P. P., Zhang, F., del Cardayre, S. B., and Keasling, J. D. (2012) Microbial engineering for the production of advanced biofuels Nature 488, 320-328 10.1038/nature11478
4. Luo, D. and Saltzman, W. M. (2000) Synthetic DNA delivery systems Nat. Biotechnol. 18, 33-37 10.1038/71889
5. Kovarik, M. L., Ornoff, D. M., Melvin, A. T., Dobes, N. C., Wang, Y. L., Dickinson, A. J., Gach, P. C., Shah, P. K., and Allbritton, N. L. (2013) Micro Total Analysis Systems: Fundamental Advances and Applications in the Laboratory, Clinic, and Field Anal. Chem. 85, 451-472 10.1021/ac3031543
6. Shih, S. C., Goyal, G., Kim, P. W., Koutsoubelis, N., Keasling, J. D., Adams, P. D., Hillson, N. J., and Singh, A. K. (2015) A Versatile Microfluidic Device for Automating Synthetic Biology ACS Synth. Biol. 4, 1151 10.1021/acssynbio.5b00062
7. Linshiz, G., Stawski, N., Goyal, G., Bi, C., Poust, S., Sharma, M., Mutalik, V., Keasling, J. D., and Hillson, N. J. (2014) PR-PR: cross-platform laboratory automation system ACS Synth. Biol. 3, 515-524 10.1021/sb4001728
8. Wolff, A., Perch-Nielsen, I. R., Larsen, U. D., Friis, P., Goranovic, G., Poulsen, C. R., Kutter, J. P., and Telleman, P. (2003) Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter Lab Chip 3, 22-27 10.1039/b209333b
9. Zare, R. N. and Kim, S. (2010) Microfluidic Platforms for Single-Cell Analysis Annu. Rev. Biomed. Eng. 12, 187-201 10.1146/annurev-bioeng-070909-105238
10. Barbulovic-Nad, I., Yang, H., Park, P. S., and Wheeler, A. R. (2008) Digital microfluidics for cell-based assays Lab Chip 8, 519-526 10.1039/b717759c
11. Inoue, H., Nojima, H., and Okayama, H. (1990) High efficiency transformation of Escherichia coli with plasmids Gene 96, 23-28 10.1016/0378-1119(90)90336-P
12. Teh, S. Y., Lin, R., Hung, L. H., and Lee, A. P. (2008) Droplet microfluidics Lab Chip 8, 198-220 10.1039/b715524g
13. Schneider, T., Kreutz, J., and Chiu, D. T. (2013) The Potential Impact of Droplet Microfluidics in Biology Anal. Chem. 85, 3476-3482 10.1021/ac400257c
14. Rosenfeld, L., Lin, T., Derda, R., and Tang, S. K. Y. (2014) Review and analysis of performance metrics of droplet microfluidics systems Microfluid. Nanofluid. 16, 921-939 10.1007/s10404-013-1310-x
15. Choi, K., Ng, A. H. C., Fobel, R., and Wheeler, A. R. (2012) Digital Microfluidics Annu. Rev. Anal. Chem. 5, 413-440 10.1146/annurev-anchem-062011-143028
16. Jebrail, M. J., Bartsch, M. S., and Patel, K. D. (2012) Digital microfluidics: a versatile tool for applications in chemistry, biology and medicine Lab Chip 12, 2452-2463 10.1039/c2lc40318h
17. Shih, S. C., Barbulovic-Nad, I., Yang, X., Fobel, R., and Wheeler, A. R. (2013) Digital microfluidics with impedance sensing for integrated cell culture and analysis Biosens. Bioelectron. 42, 314-320 10.1016/j.bios.2012.10.035
18. Au, S. H., Shih, S. C., and Wheeler, A. R. (2011) Integrated microbioreactor for culture and analysis of bacteria, algae and yeast Biomed. Microdevices 13, 41-50 10.1007/s10544-010-9469-3
19. Shih, S. C., Gach, P. C., Sustarich, J., Simmons, B. A., Adams, P. D., Singh, S., and Singh, A. K. (2015) A droplet-to-digital (D2D) microfluidic device for single cell assays Lab Chip 15, 225-236 10.1039/C4LC00794H
20. Ausubel, F. M. (2001) Current Protocols in Molecular Biology, J. Wiley, New York.
21. Au, S. H., Kumar, P., and Wheeler, A. R. (2011) A new angle on pluronic additives: advancing droplets and understanding in digital microfluidics Langmuir 27, 8586-8594 10.1021/la201185c
22. Dieckman, L., Gu, M. Y., Stols, L., Donnelly, M. I., and Collart, F. R. (2002) High throughput methods for gene cloning and expression Protein Expression Purif. 25, 1-7 10.1006/prep.2001.1602
23. Ben Yehezkel, T., Nagar, S., Mackranrs, D., Marx, Z., Linshiz, G., Shabi, U., and Shapiro, E. (2011) Computer-aided high-throughput cloning of bacteria in liquid medium BioTechniques 50, 124-127 10.2144/000113514
24. Hanahan, D. (1983) Studies on Transformation of Escherichia-Coli with Plasmids J. Mol. Biol. 166, 557-580 10.1016/S0022-2836(83)80284-8
25. Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983) Transformation of Intact Yeast-Cells Treated with Alkali Cations J. Bacteriol. 153, 163-168
26. Lubertozzi, D. and Keasling, J. D. (2009) Developing Aspergillus as a host for heterologous expression Biotechnol. Adv. 27, 53-75 10.1016/j.biotechadv.2008.09.001
27. Szewczyk, E., Nayak, T., Oakley, C. E., Edgerton, H., Xiong, Y., Taheri-Talesh, N., Osmani, S. A., and Oakley, B. R. (2006) Fusion PCR and gene targeting in Aspergillus nidulans Nat. Protoc 1, 3111-3120 10.1038/nprot.2006.405
28. Fiddes, L. K., Luk, V. N., Au, S. H., Ng, A. H., Luk, V., Kumacheva, E., and Wheeler, A. R. (2012) Hydrogel discs for digital microfluidics Biomicrofluidics 6, 14112-1411211 10.1063/1.3687381
29. Ham, T. S., Dmytriv, Z., Plahar, H., Chen, J., Hillson, N. J., and Keasling, J. D. (2012) Design, implementation and practice of JBEI-ICE: an open source biological part registry platform and tools Nucleic Acids Res. 40, e141 10.1093/nar/gks531