Cell engineering of Pseudoalteromonas haloplanktis TAC125: Construction of a mutant strain with reduced exo-proteolytic activity

Microbial Cell Factories

Volumn 5, Issue , 2006, Pages

  Parrilli, Ermenegilda ^a   Cusano, Angela Maria ^a   Giuliani, Maria ^a   Tutino, Maria Luisa ^a  

^a UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; PROTEINASE;

ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; CELL ENGINEERING; ENZYME ACTIVITY; GENE CLUSTER; GENE EXPRESSION; GENE SEQUENCE; LOW TEMPERATURE; NONHUMAN; PROTEIN DEGRADATION; PROTEIN SECRETION; PROTEIN TARGETING; PSEUDOALTEROMONAS HALOPLANKTIS;

EID: 84970005706     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-5-S1-P36     Document Type: Article

References (5)

1. Duilio A, Tutino ML, Marino G:Recombinant protein production in Antarctic Gram negativebacteria. Methods Mol Biol 2004, 267:225-237.
2. Duilio A, Marino G, Mele A, Sannia G, Tutino ML: Ufficio Italiano Brevetti e Marchi n. RM2003/A000155.
3. Tutino ML, Duilio A, Parrilli E, Remaut E, Sannia G, Marino G: A novel replication element from an Antarctic plasmid as a tool for the expression of proteins at low temperature. Extremophiles 2001, 5:257-264.
4. Médigue C, Krin E, Pascal G, Barbe V, et al.: Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. Genome Research 2005, 15:1325-35.
5. Kast P: pKSS -a second-generation general purpose cloning vector for efficient positive selection of recombinant clones. Gene 1994, 138:109-14.