Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp. PCC 6803 by overexpression of its native biosynthetic genes

Bioresource Technology

Volumn 214, Issue , 2016, Pages 761-768

  Khetkorn, Wanthanee ^a,b   Incharoensakdi, Aran ^a   Lindblad, Peter ^c   Jantaro, Saowarath ^a  

^a CHULALONGKORN UNIVERSITY   (Thailand)

^b RAJAMANGALA UNIVERSITY OF TECHNOLOGY THANYABURI   (Thailand)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Pha overexpression; Polyhydroxybutyrate; Synechocystis sp. PCC 6803

Indexed keywords

CELLS; GENES;

BIOSYNTHETIC GENE; OVER-EXPRESSION; POLY-3-HYDROXYBUTYRATE; POLY3-HYDROXYBUTYRATE (PHB); POLYHYDROXYBUTYRATE; REDUCED GROWTH RATE; SYNECHOCYSTIS SP. PCC 6803; TRANSCRIPT LEVEL;

CYTOLOGY;

NITROGEN; PHOSPHORUS; POLY(3 HYDROXYBUTYRIC ACID); ACETIC ACID DERIVATIVE; HYDROXYBUTYRIC ACID; POLY-BETA-HYDROXYBUTYRATE; POLYESTER; POLYMER;

BIOTECHNOLOGY; CYANOBACTERIUM; GENE EXPRESSION; GROWTH RATE; PLASTIC; POLYMER; SECONDARY METABOLITE;

ARTICLE; BACTERIAL STRAIN; BIOSYNTHESIS; CELL GROWTH; CELL SUSPENSION; CONTROLLED STUDY; FATTY ACID METABOLISM; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SYNECHOCYSTIS; TRANSMISSION ELECTRON MICROSCOPY; BIOTECHNOLOGY; CHEMISTRY; FLUORESCENCE MICROSCOPY; GENETIC ENGINEERING; GENETICS; METABOLISM; PH; PHOTOSYNTHESIS; PLASMID; PROTEIN DOMAIN;

SYNECHOCYSTIS; SYNECHOCYSTIS SP. PCC 6803;

ACETATES; BIOTECHNOLOGY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GENETIC ENGINEERING; HYDROGEN-ION CONCENTRATION; HYDROXYBUTYRATES; MICROSCOPY, ELECTRON, TRANSMISSION; MICROSCOPY, FLUORESCENCE; PHOTOSYNTHESIS; PLASMIDS; POLYESTERS; POLYMERS; PROTEIN DOMAINS; SYNECHOCYSTIS;

EID: 84968756942     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2016.05.014     Document Type: Article

References (30)

1. Abed R.M., Dobretsov S., Sudesh K. Applications of cyanobacteria in biotechnology. J. Appl. Microbiol. 2009, 106:1-12.
2. Allen M.M. Cyanobacterial cell inclusions. Annu. Rev. Microbiol. 1984, 38:1-25.
3. Anderson A.J., Dawes E.A. Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 1990, 54:450-472.
4. 2 recycling. Int. J. Biol. Macromol. 1999, 25:37-42.
5. 2 production and Hox-hydrogenase activity to external factors in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Int. J. Hydrogen Energy 2010, 35:6611-6616.
6. Balaji S., Gopi K., Muthuvelan B. A review on production of poly β hydroxybutyrates from cyanobacteria for the production of bioplastics. Algal Res. 2013, 2:278-285.
7. Englund E., Andersen-Ranberg J., Miao R., Hamberger B., Lindberg P. Metabolic engineering of Synechocystis sp. PCC 6803 for production of the plant diterpenoid manoyl oxide. ACS Synth. Biol. 2015, 4:1270-1278.
8. Eriksson J., Salih G.F., Ghebramedhin H., Jansson C. Deletion mutagenesis of the 5' psbA2 region in Synechocystis 6803: identification of a putative cis element involved in photoregulation. Mol. Cell. Biol. Res. Commun. 2000, 3:292-298.
9. Gründel M., Scheunemann R., Lockau W., Zilliges Y. Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803. Microbiology 2012, 158:3032-3043.
10. Hein S., Tran H., Steinbüchel A. Synechocystis sp. PCC6803 possesses a two component polyhydroxyalkanoic acid synthase similar to that of anoxygenic purple sulfur bacteria. Arch. Microbiol. 1998, 170:162-170.
11. Hondo S., Takahashi M., Osanai T., Matsuda M., Hasunuma T., Tazuke A., Nakahira Y., Chohnan S., Hasegawa M., Asayama M. Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria. J. Biosci. Bioeng. 2015, 120:510-517.
12. Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. II. Sequence determination of the entire genome and assignment of potential protein coding regions. DNA Res. 1996, 3:109-136.
13. Lindberg P., Park S., Melis A. Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab. Eng. 2010, 12:70-79.
14. MacKinney G. Absorption of light by chlorophyll solutions. J. Biol. Chem. 1941, 140:315-322.
15. Mohamed A., Eriksson J., Osiewacz H.D., Jansson C. Differential expression of the psbA genes in the cyanobacterium Synechocystis 6803. Mol. Genet. Genomics 1993, 238:161-168.
16. Monshupanee T., Incharoensakdi A. Enhanced accumulation of glycogen, lipids and polyhydroxybutyrate under optimal nutrients and light intensities in the cyanobacterium Synechocystis sp. PCC 6803. J. Appl. Microbiol. 2014, 116:830-838.
17. Osanai T., Numata K., Oikawa A., Kuwahara A., Iijima H., Doi Y., Tanaka K., Saito K., Hirai M.Y. Increased bioplastic production with an RNA polymerase sigma factor SigE during nitrogen starvation in Synechocystis sp. PCC 6803. DNA Res. 2013, 20:525-535.
18. Osanai T., Oikawa A., Lijima H., Kuwahara A., Asayama M., Tanaka K., Ikeuchi M., Saito K., Hirai M.Y. Metabolomic analysis reveals rewiring of Synechocystis sp. PCC 6803 primary metabolism by ntcA overexpression. Environ. Microbiol. 2014, 16:3304-3317.
19. Panda B., Mallick N. Enhanced poly-beta-hydroxybutyrate accumulation in a unicellular cyanobacterium, Synechocystis sp. PCC 6803. Lett. Appl. Microbiol. 2007, 44:194-198.
20. Panda B., Jain P., Sharma L., Mallick N. Optimization of cultural and nutritional conditions for accumulation of poly-beta-hydroxybutyrate in Synechocystis sp. PCC 6803. Bioresour. Technol. 2006, 97:1296-1301.
21. Schlebusch M., Forchhammer K. Requirement of the nitrogen starvation-induced protein Sll0783 for polyhydroxybutyrate accumulation in Synechocystis sp. strain PCC 6803. Appl. Environ. Microbiol. 2010, 76:6101-6107.
22. Schwarz R., Forchhammer K. Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses. Microbiology 2005, 151:2503-2514.
23. Sudesh K., Taguchi K., Doi Y. Effect of increased PHA synthetase activity on polyhydroxyalkanoates biosynthesis in Synechocystis sp. PCC 6803. Int. J. Biol. Macromol. 2002, 30:97-104.
24. Takahashi H., Miyake M., Tokiwa Y., Asada Y. Improved accumulation of poly-3 hydroxybutyrate by a recombinant cyanobacterium. Biotechnol. Lett. 1998, 20:183-186.
25. Valappil S.P., Misra S.K., Boccaccini A.R., Keshavarz T., Bucke C., Roy I. Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV. J. Biotechnol. 2007, 132:251-258.
26. Vincenzini M., Sili C., Philippis R.D., Ena A., Materassi R. Occurrence of poly-β-hydroxybutyrate in Spirulina species. J. Bacteriol. 1990, 172:2791-2792.
27. 2. Metab. Eng. 2013, 16:68-77.
28. Wu G.F., Wu Q.Y., Shen Z.Y. Accumulation of poly-β-hydroxybutyrate in cyanobacterium Synechocystis sp. PCC 6803. Bioresour. Technol. 2001, 76:85-90.
29. Wu G.F., Shen Z.Y., Wu Q.Y. Modification of carbon partitioning to enhance PHB production in Synechocystis sp. PCC 6803. Enzyme Microb. Technol. 2002, 30:710-715.
30. Yokoo T., Matsumoto K., Ooba T., Morimoto K., Taguchi S. Enhanced poly (3- hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl CoA reductase. Biosci. Biotechnol. Biochem. 2015, 79:986-988.