A pressure-driven column-based technique for the efficient extraction of DNA from respiratory samples

Clinica Chimica Acta

Volumn 445, Issue , 2015, Pages 122-126

  Hirama, Takashi ^a,b   Mogi, Hajime ^c   Egashira, Hitoshi ^a   Yamamoto, Eiji ^c   Kukisaki, Shigenari ^c   Hagiwara, Koichi ^a   Takei, Osamu ^c  

^a SAITAMA MEDICAL UNIVERSITY   (Japan)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

^c Lifetech Co Ltd   (Japan)

Author keywords

Column; DNA extraction; HIRA TAN; Pressure; Real time PCR

Indexed keywords

BACTERIAL DNA; DIAGNOSTIC KIT;

ADULT; ANALYTIC METHOD; ARTICLE; AUTOMATIC PIPETTING DEVICE; BACTERIUM IDENTIFICATION; CELL COUNT; CENTRIFUGATION; CHEMISTRY; CLINICAL ARTICLE; CONTROLLED STUDY; DEVICES; DIAGNOSIS; DIAGNOSTIC KIT; DNA CONCENTRATION; DNA EXTRACTION; DNA PURITY; DNA QUANTITY; GENE AND NUCLEIC ACID PARAMETERS; GENE DOSAGE; GROWTH, DEVELOPMENT AND AGING; HAEMOPHILUS INFLUENZAE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; ISOLATION AND PURIFICATION; KLEBSIELLA PNEUMONIAE; LABORATORY DEVICE; MICROBIOLOGY; MORAXELLA CATARRHALIS; PATHOGENICITY; PNEUMONIA, BACTERIAL; PRESSURE; PRESSURE DRIVEN COLUMN BASED TECHNIQUE; PRIORITY JOURNAL; PROCEDURES; PSEUDOMONAS AERUGINOSA; REAL TIME POLYMERASE CHAIN REACTION; SOLID PHASE EXTRACTION; SPUTUM; STREPTOCOCCUS PNEUMONIAE;

CENTRIFUGATION; CHEMISTRY; DIAGNOSIS; DNA, BACTERIAL; GROWTH AND DEVELOPMENT; HAEMOPHILUS INFLUENZAE; HUMANS; INSTRUMENTATION; ISOLATION AND PURIFICATION; KLEBSIELLA PNEUMONIAE; METHODS; MICROBIOLOGY; MORAXELLA (BRANHAMELLA) CATARRHALIS; PATHOGENICITY; PNEUMONIA, BACTERIAL; PRESSURE; PSEUDOMONAS AERUGINOSA; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; SOLID PHASE EXTRACTION; SPUTUM; STREPTOCOCCUS PNEUMONIAE;

EID: 84964310691     PISSN: 00098981     EISSN: 18733492     Source Type: Journal    
DOI: 10.1016/j.cca.2015.03.023     Document Type: Article

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