Three-in-one enzyme assay based on single molecule detection in femtoliter arrays

Analytical and Bioanalytical Chemistry

Volumn 407, Issue 24, 2021, Pages

  Liebherr, Raphaela B ^a   Hutterer, Albert ^b   Mickert, Matthias J ^a   Vogl, Franziska C ^a   Beutner, Andrea ^a   Lechner, Alfred ^b   Hummel, Helmut ^b   Gorris, Hans H ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF APPLIED SCIENCES REGENSBURG   (Germany)

Author keywords

Arrays; Digital concentration readout; Enzyme kinetics; Fluorescence microscopy; Single molecule detection

Indexed keywords

ENZYME KINETICS; ETCHING; FLUORESCENCE; FLUORESCENCE MICROSCOPY; FUSED SILICA; MOLECULES; POLYDIMETHYLSILOXANE; SILICONES;

ARRAYS; BIOANALYTICAL APPLICATIONS; CONFORMATIONAL HETEROGENEITY; DIGITAL CONCENTRATION READOUT; ENZYME CONCENTRATIONS; FLUOROGENIC REACTIONS; POLYDIMETHYLSILOXANE PDMS; SINGLE-MOLECULE DETECTION;

ENZYME ACTIVITY;

BETA GALACTOSIDASE; DIMETICONE;

CHEMISTRY; LIMIT OF DETECTION; METABOLISM;

BETA-GALACTOSIDASE; DIMETHYLPOLYSILOXANES; LIMIT OF DETECTION;

EID: 84963560586     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-015-8910-0     Document Type: Article

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