Purification of lysosomes using supraparamagnetic iron oxide nanoparticles (SPIONs)

Cold Spring Harbor Protocols

Volumn 2016, Issue 4, 2016, Pages 338-342

  Rofe, Adam P ^a   Pryor, Paul R ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

IRON DEXTRAN; SUPERPARAMAGNETIC IRON OXIDE NANOPARTICLE; TISSUE CULTURE MEDIUM; FERRIC ION; FERRIC OXIDE; NANOPARTICLE;

ANIMAL CELL; ARTICLE; CELL FUSION; CELL LYSATE; ENDOSOME; FLOW CYTOMETRY; FRACTIONATION; ISOLATION AND PURIFICATION; LYSOSOME PURIFICATION; MACROPHAGE CULTURE; MOUSE; NONHUMAN; REACTION OPTIMIZATION; ULTRACENTRIFUGATION; ANIMAL; CELL FRACTIONATION; CELL LINE; ENDOCYTOSIS; LYSOSOME; MACROPHAGE; MAGNETISM; METABOLISM; PROCEDURES;

ANIMALS; CELL FRACTIONATION; CELL LINE; ENDOCYTOSIS; FERRIC COMPOUNDS; LYSOSOMES; MACROPHAGES; MAGNETICS; MICE; NANOPARTICLES;

EID: 84962449800     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot084822     Document Type: Article

References (3)

1. Diettrich O, Mills K, Johnson AW, Hasilik A, Winchester BG. 1998. Application of magnetic chromatography to the isolation of lysosomes from fibroblasts of patients with lysosomal storage disorders. FEBS Lett 441: 369–372.
2. Pryor PR. 2014. Isolating lysosomes from rat liver. Cold Spring Harb Protoc doi: 10.1101/pdb.prot084822.
3. Rodriguez-Paris JM, Nolta KV, Steck TL. 1993. Characterization of lysosomes isolated from Dictyostelium discoideum by magnetic fractionation. J Biol Chem 268: 9110–9116.