A spectroscopic method to determine the activity of the restriction endonuclease EcoRV that involves a single reaction

Analytical Biochemistry

Volumn 497, Issue , 2016, Pages 103-105

  Huang, Qing ^a   Quiñones, Edwin ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

[No Author keywords available]

Indexed keywords

SPECTROSCOPIC ANALYSIS;

DNA SUBSTRATES; FLUORESCENCE INTENSITIES; MICHAELIS-MENTEN; MOLECULAR BEACON; RESTRICTION ENDONUCLEASES; RESTRICTION ENZYMES; SPECTROSCOPIC METHOD; TARGET SEQUENCES;

REACTION KINETICS;

DNA; RESTRICTION ENDONUCLEASE; RESTRICTION ENDONUCLEASE ECORV; UNCLASSIFIED DRUG; FLUORESCENT DYE; GATATC-SPECIFIC TYPE II DEOXYRIBONUCLEASES; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICHAELIS CONSTANT; MICHAELIS MENTEN KINETICS; MOLECULAR BEACON; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN SECONDARY STRUCTURE; SPECTROSCOPY; SUBSTRATE CONCENTRATION; CHEMISTRY; ENZYME SPECIFICITY; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PROCEDURES; SPECTROFLUOROMETRY;

BASE SEQUENCE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; ENZYME ASSAYS; FLUORESCENT DYES; KINETICS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

EID: 84959552157     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.04.038     Document Type: Article

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