Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial

Journal of Clinical Microbiology

Volumn 54, Issue 3, 2016, Pages 687-698

  Salimnia, Hossein ^a,b   Fairfax, Marilynn R ^a,b   Lephart, Paul R ^b   Schreckenberger, Paul ^c   DesJarlais, Sharon M ^c   Johnson, J Kristie ^d   Robinson, Gwen ^d   Carroll, Karen C ^e   Greer, Amy ^e   Morgan, Margie ^f   Chan, Raymond ^f   Loeffelholz, Michael ^g   Valencia Shelton, Frances ^g,k   Jenkins, Stephen ^h   Schuetz, Audrey N ^h   Daly, Judy A ⁱ   Barney, Trenda ⁱ   Hemmert, Andrew ^j   Kanack, Kristen J ^j  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b DMC University Laboratories   (United States)

^c LOYOLA UNIVERSITY MEDICAL CENTER   (United States)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^e JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^f CEDARS SINAI MEDICAL CENTER   (United States)

^g University of Texas Medical Branch School of Medicine   (United States)

^h WEILL CORNELL MEDICAL CENTER   (United States)

ⁱ PRIMARY CHILDREN'S MEDICAL CENTER   (United States)

^j Idaho Technology Inc   (United States)

^k STRONG MEMORIAL HOSPITAL   (United States)

more..

Author keywords

[No Author keywords available]

Indexed keywords

PENICILLIN BINDING PROTEIN 2A;

ACINETOBACTER BAUMANNII; ACINETOBACTER CALCOACETICUS-BAUMANNII COMPLEX; ADULT; ANTIBIOTIC RESISTANCE; ARTICLE; AUTOMATION; BLA KPC GENE; BLOOD CULTURE; BLOOD CULTURE IDENTIFICATION; CHILD; GENE; HUMAN; KLEBSIELLA OXYTOCA; MAJOR CLINICAL STUDY; MECA GENE; MICROBIAL IDENTIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEPSIS; VANA/B GENE; ANTIFUNGAL RESISTANCE; BACTERIAL GENE; BACTERIUM; CLASSIFICATION; DRUG EFFECTS; FUNGAL GENE; GENETICS; MICROBIOLOGY; MULTIPLEX POLYMERASE CHAIN REACTION; REPRODUCIBILITY; YEAST;

BACTERIA; DRUG RESISTANCE, BACTERIAL; DRUG RESISTANCE, FUNGAL; GENES, BACTERIAL; GENES, FUNGAL; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEPSIS; YEASTS;

EID: 84959548995     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01679-15     Document Type: Article

References (32)

1. Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, Sevransky JE, Sprung CL, Douglas IS, Jaeschke R, Osborn TM, Nunnally ME, Townsend SR, Reinhart K, Kleinpell RM, Angus DC, Deutschman CS, Machado FR, Rubenfeld GD, Webb S, Beale RJ, Vincent J-L, Moreno R. 2013. Surviving Sepsis Campaign: International guidelines for management of severe sepsis and septic shock, 2012. Intensive CareMed39:165-228. http://dx.doi.org/10.1007/s00134-012-2769-8.
2. Hall MJ, Williams SN, DeFrances CJ, Golosinskiy A. 2011. Inpatient care for septicemia or sepsis: A challenge for patients and hospitals. NCHS Data Brief 62:1-8.
3. Angus DC, Linde-Zwirble WT, Lidicker J, Clermont G, Carcillo J, Pinsky MR. 2001. Epidemiology of severe sepsis in the United States: Analysis of incidence, outcome, and associated costs of care. Crit Care Med 29:1303-1310. http://dx.doi.org/10.1097/00003246-200107000-00002.
4. Spellberg B, Guidos R, Gilbert D, Bradley J, Boucher HW, Scheld WM, Bartlett JG, Edwards J. 2008. The epidemic of antibiotic-resistant infections: A call to action for the medical community from the Infectious Diseases Society of America. Clin Infect Dis 46:155-164. http://dx.doi.org/10.1086/524891.
5. Perez KK, Olsen RJ, Musick WL, Cernoch PL, Davis JR, Land GA, Peterson LE, Musser JM. 2013. Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs. Arch Pathol Lab Med 137:1247-1254. http://dx.doi.org/10.5858/arpa.2012-0651-OA.
6. MacArthur RD, Miller M, Albertson T, Panacek E, Johnson D, Teoh L, Barchuk W. 2004. Adequacy of early empiric antibiotic treatment and survival in severe sepsis: Experience from the MONARCS trial. Clin Infect Dis 38:284-288. http://dx.doi.org/10.1086/379825.
7. Garnacho-Montero J, Ortiz-Leyba C, Herrera-Melero I, Aldabó-Pallás T, Cayuela-Dominguez A, Marquez-Vacaro JA, Carbajal-Guerrero J, Garcia-Garmendia JL. 2008. Mortality and morbidity attributable to inadequate empirical antimicrobial therapy in patients admitted to the ICU with sepsis: A matched cohort study. J Antimicrob Chemother 61:436-441.
8. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, Suppes R, Feinstein D, Zanotti S, Taiberg L, Gurka D, Kumar A, Cheang M. 2006. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34:1589-1596. http://dx.doi.org/10.1097/01.CCM.0000217961 .75225.E9.
9. Dierkes C, Ehrenstein B, Siebig S, Linde H-J, Reischl U, Salzberger B. 2009. Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 9:126. http://dx.doi.org/10.1186/1471-2334-9-126.
10. Leibovici L, Shraga I, Drucker M, Konigsberger H, Samra Z, Pitlik SD. 1998. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J Intern Med 244:379-386. http://dx.doi.org/10.1046/j.1365-2796.1998.00379.x.
11. Ly T, Gulia J, Pyrgos V, Waga M, Shoham S. 2008. Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time. Ther Clin Risk Manag 4:637-640.
12. Frye AM, Baker CA, Rustvold DL, Heath KA, Hunt J, Leggett JE, Oethinger M. 2012. Clinical impact of a real-time PCR assay for rapid identification of staphylococcal bacteremia. J Clin Microbiol 50:127-133. http://dx.doi.org/10.1128/JCM.06169-11.
13. Forrest GN, Roghmann M-C, Toombs LS, Johnson JK, Weekes E, Lincalis DP, Venezia RA. 2008. Peptide nucleic acid fluorescent in situ hybridization for hospital-acquired enterococcal bacteremia: Delivering earlier effective antimicrobial therapy. Antimicrob Agents Chemother 52: 3558-3563. http://dx.doi.org/10.1128/AAC.00283-08.
14. Poritz MA, Blaschke AJ, Byington CL, Meyers L, Nilsson K, Jones DE, Thatcher SA, Robbins T, Lingenfelter B, Amiott E, Herbener A, Daly J, Dobrowolski SF, Teng DH-F, Ririe KM. 2011. FilmArray, and automated nested multiplex PCR system for multi-pathogen detection: Development and application to respiratory tract infection. PLoS One 6:e26047. http://dx.doi.org/10.1371/journal.pone.0026047.
15. Blaschke AJ, Heyrend C, Byington Cl Fisher MA, Barker E, Garrone NF, Thatcher SA, Pavia AT, Barney T, Garrison DA, Daly JA, Ririe KM, Ofta I, Poritz MA. 2012. Rapid identification of blood cultures by multiplex PCR using the FilmArray system. Diagn Microbiol Infect Dis 74: 349-355. http://dx.doi.org/10.1016/j.diagmicrobio.2012.08.013.
16. Clinical and Laboratory Standards Institute (CLSI). 2007. Principles and procedures for blood cultures: Approved guideline. Document M47-A, vol. 27. Clinical and Laboratory Standards Institute, Wayne, PA.
17. Schreckenberger PC, Daneshvar MI, Hollis DG. 2007. Acinetobacter, Achromobacter, Chryseobacterium, Moraxella, and other nonfermentative Gram-negative rods, p 770-802. In Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (ed), Manual of clinical microbiology, 9th ed. ASM Press, Washington, DC.
18. Food and Drug Administration. 510(k) substantial equivalence determination decision summary assay and instrument combination template. Food and Drug Administration, Rockville, MD. http://www.accessdata .fda.gov/cdrh-docs/reviews/K130914.pdf.
19. Clopper CJ, Pearson ES. 1934. The use of confidence or fiducial limits illustrated in the case of the binomial. Biometrika 26:404. http://dx.doi.org/10.1093/biomet/26.4.404.
20. Granier SA, Leflon-Guibout V, Goldstein FW, Nicolas-Chanoine M-H. 2003. Enterobacterial repetitive intergenic consensus 1R PCR assay for detection of Raoultella sp. isolates among strains identified as Klebsiella oxytoca in the clinical laboratory. J Clin Microbiol 41:1740-1742. http://dx.doi.org/10.1128/JCM.41.4.1740-1742.2003.
21. Park JS, Hong KH, Lee HJ, Choi SH, Song SH, Song K-H, Kim HB, Park KU, Song J, Kim E-C. 2011. Evaluation of three phenotypic identification systems for clinical isolates of Raoultella ornithinolytica. J Med Microbiol 60:492-499. http://dx.doi.org/10.1099/jmm.0.020768-0.
22. Kollef MH. 2008. Broad-spectrum antimicrobials and the treatment of serious bacterial infections: Getting it right up front. Clin Infect Dis 47(Suppl 1):S3-S13. http://dx.doi.org/10.1086/590061.
23. Kumar A, Ellis P, Arabi Y, Roberts D, Light B, Parrillo JE, Dodek P, Wood G, Kumar A, Simon D, Peters C, Ahsan M, Chateau D, Cooperative Antimicrobial Therapy of Septic Shock Database Research Group. 2009. Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest 136:1237-1248. http://dx.doi.org/10.1378/chest.09-0087.
24. Southern TR, VanSchooneveld TC, Bannister DL, Brown TL, Crismon AS, Buss SN, Iwen PC, Fey PD. 2015. Implementation and performance of the BioFire FilmArray blood culture identification panel with antimicrobial treatment recommendations for bloodstream infections at a midwestern academic tertiary hospital. Diagn Microbiol Infect Dis 81:96-101. http://dx.doi.org/10.1016/j.diagmicrobio.2014.11.004.
25. Poirel L, Nordmann P, Lagrutta E. 2010. Emergence of KPC-producing Pseudomonas aeruginosa in the United States. Antimicrob Agents Chemother 54:3072. http://dx.doi.org/10.1128/AAC.00513-10.
26. Robledo IE, Aquino EE, Vázquez GJ. 2011. Detection of the KPC gene in Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii during a PCR-based nosocomial surveillance study in Puerto Rico. Antimicrob Agents Chemother 55:2968-2970. http://dx.doi.org/10.1128/AAC.01633-10.
27. Altun O, Almuhayawi M, Ullberg M, Ozenci V. 2013. Clinical evaluation of the FilmArray blood culture identification panel in identification of bacteria and yeasts from positive blood culture bottles. J Clin Microbiol 51:4130-4136. http://dx.doi.org/10.1128/JCM.01835-13.
28. Bhatti MM, Boonlayangoor S, Beavis KG, Tesic V. 2014. Evaluation of FilmArray and Verigene systems for rapid identification of positive blood cultures. J Clin Microbiol 52:3433-3436. http://dx.doi.org/10.1128/JCM .01417-14.
29. Rand KH, Delano JP. 2014. Direct identification of bacteria in positive blood cultures: Comparison of two rapid methods, FilmArray and mass spectrometry. Diagn Microbiol Infect Dis 79:293-297. http://dx.doi.org/10.1016/j.diagmicrobio.2014.03.014.
30. Zheng X, Polanco W, Carter D, Schulman S. 2014. Rapid identification of pathogens from pediatric blood cultures by use of the FilmArray blood culture identification panel. J Clin Microbiol 52:4368-4371. http://dx.doi.org/10.1128/JCM.02133-14.
31. Nagel JL, Huang AM, Kunapuli A, Gandhi TN, Washer LL, Lassiter J, Patel T, Newton DW. 2014. Impact of antimicrobial stewardship intervention on coagulase-negative Staphylococcus blood cultures in conjunction with rapid diagnostic testing. J Clin Microbiol 52:2849-2854. http://dx.doi.org/10.1128/JCM.00682-14.
32. Chang S, Sievert DM, Hageman JC, Boulton ML, Tenover FC, Downes FP, Shah S, Rudrik JT, Pupp GR, Brown WJ, Cardo D, Fridkin SK. 2003. Vancomycin-resistant Staphylococcus aureus Investigative Team. Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. N Engl J Med 14:1342-1347.