A Fast and practical yeast transformation method mediated by escherichia coli based on a trans-kingdom conjugal transfer system: Just mix two cultures and wait one hour

PLoS ONE

Volumn 11, Issue 2, 2016, Pages

  Moriguchi, Kazuki ^a   Yamamoto, Shinji ^a   Ohmine, Yuta ^a   Suzuki, Katsunori ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DITHIOTHREITOL; THIOL; BACTERIAL DNA;

ARTICLE; BACTERIAL CONJUGAL TRANSFER SYSTEM; BACTERIUM CULTURE; COCULTURE; CONJUGATION; CONTROLLED STUDY; DNA TRANSFER; DNA VECTOR; ESCHERICHIA COLI; EUKARYOTIC CELL; FUNGAL CELL CULTURE; FUNGAL PHENOMENA AND FUNCTIONS; FUNGAL STRAIN; MICROBIOLOGICAL PHENOMENA AND FUNCTIONS; MIXED CELL CULTURE; NONHUMAN; PLASMID; SACCHAROMYCES CEREVISIAE; SHUTTLE VECTOR; TRANS KINGDOM CONJUGATION; YEAST TRANSFORMATION; GENE TRANSFER; GENE VECTOR; GENETICS;

DNA, BACTERIAL; ESCHERICHIA COLI; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; SACCHAROMYCES CEREVISIAE;

EID: 84959079706     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0148989     Document Type: Article

References (16)

1. Moriguchi K, Yamamoto S, Tanaka K, Kurata N, Suzuki K. Trans-Kingdom horizontal DNA transfer from bacteria shows large plasticity by defects in nonessential, polymorphic genes of the recipient eukaryote. PLOS ONE. 2013; 8: e74590.
2. Moriguchi K, Edahiro N, Yamamoto S, Tanaka K, Kurata N, Suzuki K. Trans-kingdom genetic transfer from Escherichia coli to Saccharomyces cerevisiae as a simple gene introduction tool. Appl Environ Microbiol. 2013; 79: 4393-4400. doi: 10.1128/AEM.00770-13 PMID: 23666333
3. Heinemann JA, Sprague GF Jr. Bacterial conjugative plasmids mobilize DNA transfer between bacteria and yeast. Nature. 1989; 340: 205-209. PMID: 2666856
4. Nishikawa M, Suzuki K, Yoshida K. Structural and functional stability of IncP plasmids during stepwise transmission by trans-kingdom mating: promiscuous conjugation of Escherichia coli and Saccharomyces cerevisiae. Jpn J Genet. 1990; 65: 323-334. PMID: 2248784
5. Bates S, Cashmore AM, Wilkins BM. IncP plasmids are unusually effective in mediating conjugation of Escherichia coli and Saccharomyces cerevisiae: involvement of the tra2 mating system. J Bacteriol. 1998; 180: 6538-6543. PMID: 9851996
6. Mizuta M, Satoh E, Katoh C, Tanaka K, Moriguchi K, Suzuki K. Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon. Microbiol Res. 2012; 167: 311-316. doi: 10.1016/j.micres.2011.10.001 PMID: 22169356
7. Nishikawa M, Suzuki K, Yoshida K. DNA integration into recipient yeast chromosomes by trans-kingdom conjugation between Escherichia coli and Saccharomyces cerevisiae. Curr Genet. 1992 21: 101-108. PMID: 1568253
8. Nishikawa M, Yoshida K. Trans-kingdom conjugation offers a powerful gene targeting: tool in yeast. Genet Anal. 1998; 14: 65-73. PMID: 9526697
9. Suzuki K, Yanagishima N. A mutation leading to constitutive expression of high sexual activity in the yeast Saccharomyces cerevisiae. Plant Cell Phys. 1986; 27: 523-531.
10. Simon R, Priefer U, Pühler A. A broad host-range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Nat Biotech. 1983; 1: 784-791.
11. Ito H, Murata K, Kimura A. Transformation of Intact Yeast Cells Treated with Alkali Cations or Thiol Compounds. Agric Biol Chem. 1984; 48: 341-347-43.
12. Thompson JR, Register E, Curotto J, Kurtz M, Kelly R. An Improved Protocol for The Preparation of Yeast Cells for Transformation by Electroporation. Yeast. 1998; 14: 565-571. PMID: 9605506
13. Brzobohatý B, KovÁČ L. Factors Enhancing Genetic Transformation of Intact Yeast Cells Modify Cell Wall Porosity. Microbiology. 1986; 132: 3089-3093.
14. Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983; 153: 163-168. PMID: 6336730
15. Gietz RD, Schiestl RH. Quick and Easy Yeast Transformation Using the LiAc/SS Carrier DNA/PEG Method. Nat Protocols. 2007; 2:35-37. PMID: 17401335
16. Kawai S, Pham TA, Nguyen HT, Nankai H, Utsumi T, Fukuda Y, Murata K. Molecular insights on DNA delivery into Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2004; 317: 100-107. PMID: 15047153