A high-content platform to characterise human induced pluripotent stem cell lines

Methods

Volumn 96, Issue , 2016, Pages 85-96

  Leha, Andreas ^c   Moens, Nathalie ^a   Meleckyte, Ruta ^a   Culley, Oliver J ^a   Gervasio, Mia K ^a   Kerz, Maximilian ^a,b   Reimer, Andreas ^a   Cain, Stuart A ^e   Streeter, Ian ^d   Folarin, Amos ^b   Stegle, Oliver ^d   Kielty, Cay M ^e   Durbin, Richard ^c   Watt, Fiona M ^a   Danovi, Davide ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

^c WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^d WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^e UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Cell based assays; High content; Human pluripotent stem cells; Induced pluripotent stem cells; IPSCs; Phenotype screening

Indexed keywords

FIBRONECTIN; PEPTIDE;

ARTICLE; CELL ADHESION; CELL COUNT; CELL POPULATION; CELL PROLIFERATION; CELL STRUCTURE; CONTROLLED STUDY; DISEASE MODEL; DRUG DEVELOPMENT; HIGH CONTENT SCREENING; HUMAN; HUMAN CELL; HUMAN DEVELOPMENT; PHENOTYPE; PHENOTYPIC VARIATION; PLURIPOTENT STEM CELL; PLURIPOTENT STEM CELL LINE; PRIORITY JOURNAL; SCREENING; STEM CELL TRANSPLANTATION; VARIANCE; AMINO ACID SEQUENCE; CELL DIFFERENTIATION; CELL LINE; CHEMISTRY; CYTOLOGY; FEEDER CELL; HIGH THROUGHPUT SCREENING; INDUCED PLURIPOTENT STEM CELL; METABOLISM; MOLECULAR GENETICS; MOLECULAR IMAGING; PROCEDURES; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; CELL ADHESION; CELL DIFFERENTIATION; CELL LINE; FEEDER CELLS; FIBRONECTINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MOLECULAR IMAGING; MOLECULAR SEQUENCE DATA; PEPTIDES; PHENOTYPE;

EID: 84958165943     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.11.012     Document Type: Article

References (27)

1. Yamanaka S. Induced pluripotent stem cells: past, present, and future. Cell Stem Cell 2012, 10(6):678-684.
2. Narsinh K.H., et al. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells. J. Clin. Invest. 2011, 121(3):1217-1221.
3. Koyanagi-Aoi M., et al. Differentiation-defective phenotypes revealed by large-scale analyses of human pluripotent stem cells. Proc. Natl. Acad. Sci. USA 2013, 110(51):20569-20574.
4. Rouhani F., et al. Genetic background drives transcriptional variation in human induced pluripotent stem cells. PLoS Genet. 2014, 10(6):e1004432.
5. Cacchiarelli D., et al. Integrative analyses of human reprogramming reveal dynamic nature of induced pluripotency. Cell 2015, 162(2):412-424.
6. Ruiz S., et al. Identification of a specific reprogramming-associated epigenetic signature in human induced pluripotent stem cells. Proc. Natl. Acad. Sci. USA 2012, 109(40):16196-16201.
7. Yusa K., et al. Targeted gene correction of alpha1-antitrypsin deficiency in induced pluripotent stem cells. Nature 2011, 478(7369):391-394.
8. Laufer C., et al. Measuring genetic interactions in human cells by RNAi and imaging. Nat. Protoc. 2014, 9(10):2341-2353.
9. Fusaki N., et al. Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 2009, 85(8):348-362.
10. Muller F.J., et al. A bioinformatic assay for pluripotency in human cells. Nat. Methods 2011, 8(4):315-317.
11. Robinton D.A., Daley G.Q. The promise of induced pluripotent stem cells in research and therapy. Nature 2012, 481(7381):295-305.
12. Rashid S.T., et al. Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells. J. Clin. Invest. 2010, 120(9):3127-3136.
13. Danovi D., et al. High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines. Methods Enzymol. 2012, 506:311-329.
14. Viswanathan P., et al. Human pluripotent stem cells on artificial microenvironments: a high content perspective. Front. Pharmacol. 2014, 5:150.
15. Watt F.M., Huck W.T. Role of the extracellular matrix in regulating stem cell fate. Nat. Rev. Mol. Cell. Biol. 2013, 14(8):467-473.
16. Pulina M., Liang D., Astrof S. Shape and position of the node and notochord along the bilateral plane of symmetry are regulated by cell-extracellular matrix interactions. Biol. Open 2014, 3(7):583-590.
17. Stoffels J.M., Zhao C., Baron W. Fibronectin in tissue regeneration: timely disassembly of the scaffold is necessary to complete the build. Cell. Mol. Life Sci. 2013, 70(22):4243-4253.
18. Abraham S., et al. Characterization of human fibroblast-derived extracellular matrix components for human pluripotent stem cell propagation. Acta Biomater. 2010, 6(12):4622-4633.
19. Andrews P.D., et al. High-content screening of feeder-free human embryonic stem cells to identify pro-survival small molecules. Biochem. J. 2010, 432(1):21-33.
20. Kalaskar D.M., et al. Characterization of the interface between adsorbed fibronectin and human embryonic stem cells. J. R. Soc. Interface 2013, 10(83):20130139.
21. Kim M.H., Kino-oka M. Maintenance of undifferentiated state of human induced pluripotent stem cells through cytoskeleton-driven force acting to secreted fibronectin on a dendrimer-immobilized surface. J. Biosci. Bioeng. 2014, 118(6):716-722.
22. Soteriou D., et al. Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance. J. Biol. Chem. 2013, 288(26):18716-18731.
23. Yamasaki S., et al. Generation of human induced pluripotent stem (Ips) cells in serum- and feeder-free defined culture and TGF-Beta1 regulation of pluripotency. PLoS One 2014, 9(1):e87151.
24. Llopis-Hernandez V., et al. Role of material-driven fibronectin fibrillogenesis in protein remodeling. Biores. Open Access 2013, 2(5):364-373.
25. To W.S., Midwood K.S. Plasma and cellular fibronectin: distinct and independent functions during tissue repair. Fibrogenesis Tissue Repair 2011, 4:21.
26. Ghule P.N., et al. Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells. J. Cell. Physiol. 2011, 226(5):1149-1156.
27. Sero J.E., et al. Cell shape and the microenvironment regulate nuclear translocation of NF-kappaB in breast epithelial and tumor cells. Mol. Syst. Biol. 2015, 11(3):790.