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Volumn 9, Issue 1, 2016, Pages

A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia

Author keywords

Dorsal root ganglia (DRG); Peripheral neuropathy; Primary culture; Sensory neuron

Indexed keywords

ANIMAL; C57BL MOUSE; DISSECTION; MALE; PROCEDURES; SPINAL CORD; SPINAL GANGLION; SURGERY;

EID: 84957837284     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/s13104-016-1915-8     Document Type: Article
Times cited : (102)

References (37)
  • 1
    • 0033044540 scopus 로고    scopus 로고
    • Formation of lamina-specific synaptic connections
    • 1:CAS:528:DyaK1MXhvVWiur4%3D 10072367
    • Sanes JR, Yamagata M. Formation of lamina-specific synaptic connections. Curr Opin Neurobiol. 1999;9(1):79-87.
    • (1999) Curr Opin Neurobiol , vol.9 , Issue.1 , pp. 79-87
    • Sanes, J.R.1    Yamagata, M.2
  • 2
    • 84861704266 scopus 로고    scopus 로고
    • Molecular interactions underlying the specification of sensory neurons
    • 1:CAS:528:DC%2BC38XlvVSmt7w%3D 22516617
    • Lallemend F, Ernfors P. Molecular interactions underlying the specification of sensory neurons. Trends Neurosci. 2012;35(6):373-81.
    • (2012) Trends Neurosci , vol.35 , Issue.6 , pp. 373-381
    • Lallemend, F.1    Ernfors, P.2
  • 3
    • 35148891631 scopus 로고    scopus 로고
    • The somatosensory system, with emphasis on structures important for pain
    • 17604109
    • Willis WD Jr. The somatosensory system, with emphasis on structures important for pain. Brain Res Rev. 2007;55(2):297-313.
    • (2007) Brain Res Rev , vol.55 , Issue.2 , pp. 297-313
    • Willis, W.D.1
  • 4
    • 0021895384 scopus 로고
    • Conduction velocity is related to morphological cell type in rat dorsal root ganglion neurones
    • 1:STN:280:DyaL2M3htVGksg%3D%3D 3999040 1193363
    • Harper AA, Lawson SN. Conduction velocity is related to morphological cell type in rat dorsal root ganglion neurones. J Physiol. 1985;359:31-46.
    • (1985) J Physiol , vol.359 , pp. 31-46
    • Harper, A.A.1    Lawson, S.N.2
  • 5
    • 0029034576 scopus 로고
    • Immunocytochemical localization of trkA receptors in chemically identified subgroups of adult rat sensory neurons
    • 1:STN:280:DyaK28%2FjsFChsA%3D%3D 7551174 2758238
    • Averill S, McMahon SB, Clary DO, Reichardt LF, Priestley JV. Immunocytochemical localization of trkA receptors in chemically identified subgroups of adult rat sensory neurons. Eur J Neurosci. 1995;7(7):1484-94.
    • (1995) Eur J Neurosci , vol.7 , Issue.7 , pp. 1484-1494
    • Averill, S.1    McMahon, S.B.2    Clary, D.O.3    Reichardt, L.F.4    Priestley, J.V.5
  • 6
    • 0032821688 scopus 로고    scopus 로고
    • Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro
    • 1:STN:280:DC%2BD3c%2Fjt1WqsQ%3D%3D 10564348
    • Gavazzi I, Kumar RD, McMahon SB, Cohen J. Growth responses of different subpopulations of adult sensory neurons to neurotrophic factors in vitro. Eur J Neurosci. 1999;11(10):3405-14.
    • (1999) Eur J Neurosci , vol.11 , Issue.10 , pp. 3405-3414
    • Gavazzi, I.1    Kumar, R.D.2    McMahon, S.B.3    Cohen, J.4
  • 7
    • 84899152535 scopus 로고    scopus 로고
    • The functional and anatomical dissection of somatosensory subpopulations using mouse genetics
    • 24795573 4001001
    • Le Pichon CE, Chesler AT. The functional and anatomical dissection of somatosensory subpopulations using mouse genetics. Front Neuroanat. 2014;8:21.
    • (2014) Front Neuroanat , vol.8 , pp. 21
    • Le Pichon, C.E.1    Chesler, A.T.2
  • 9
    • 7044246002 scopus 로고    scopus 로고
    • Nogo-66 receptor prevents raphespinal and rubrospinal axon regeneration and limits functional recovery from spinal cord injury
    • 1:CAS:528:DC%2BD2cXpvVChs7s%3D 15504325
    • Kim JE, Liu BP, Park JH, Strittmatter SM. Nogo-66 receptor prevents raphespinal and rubrospinal axon regeneration and limits functional recovery from spinal cord injury. Neuron. 2004;44(3):439-51.
    • (2004) Neuron , vol.44 , Issue.3 , pp. 439-451
    • Kim, J.E.1    Liu, B.P.2    Park, J.H.3    Strittmatter, S.M.4
  • 10
    • 33749620373 scopus 로고    scopus 로고
    • Rab5 and Rab7 control endocytic sorting along the axonal retrograde transport pathway
    • 1:CAS:528:DC%2BD28Xht1WhtbjP 17046692
    • Deinhardt K, Salinas S, Verastegui C, Watson R, Worth D, Hanrahan S, Bucci C, Schiavo G. Rab5 and Rab7 control endocytic sorting along the axonal retrograde transport pathway. Neuron. 2006;52(2):293-305.
    • (2006) Neuron , vol.52 , Issue.2 , pp. 293-305
    • Deinhardt, K.1    Salinas, S.2    Verastegui, C.3    Watson, R.4    Worth, D.5    Hanrahan, S.6    Bucci, C.7    Schiavo, G.8
  • 11
    • 33644874107 scopus 로고    scopus 로고
    • Semaphorin 3A and neurotrophins: a balance between apoptosis and survival signaling in embryonic DRG neurons
    • 1:CAS:528:DC%2BD28XntVShtQ%3D%3D 16336628
    • Ben-Zvi A, Yagil Z, Hagalili Y, Klein H, Lerman O, Behar O. Semaphorin 3A and neurotrophins: a balance between apoptosis and survival signaling in embryonic DRG neurons. J Neurochem. 2006;96(2):585-97.
    • (2006) J Neurochem , vol.96 , Issue.2 , pp. 585-597
    • Ben-Zvi, A.1    Yagil, Z.2    Hagalili, Y.3    Klein, H.4    Lerman, O.5    Behar, O.6
  • 12
    • 70349408772 scopus 로고    scopus 로고
    • Dorsal root ganglia sensory neuronal cultures: a tool for drug discovery for peripheral neuropathies
    • 1:CAS:528:DC%2BD1MXhtFOiu7bI 20657751 2908326
    • Melli G, Höke A. Dorsal root ganglia sensory neuronal cultures: a tool for drug discovery for peripheral neuropathies. Expert Opin Drug Discov. 2009;4(10):1035-45.
    • (2009) Expert Opin Drug Discov , vol.4 , Issue.10 , pp. 1035-1045
    • Melli, G.1    Höke, A.2
  • 13
    • 38649110117 scopus 로고    scopus 로고
    • Hyperglycaemia inhibits Schwann cell proliferation and migration and restricts regeneration of axons and Schwann cells from adult murine DRG
    • 1:CAS:528:DC%2BD1cXhsVyitLs%3D 18024075
    • Gumy LF, Bampton ET, Tolkovsky AM. Hyperglycaemia inhibits Schwann cell proliferation and migration and restricts regeneration of axons and Schwann cells from adult murine DRG. Mol Cell Neurosci. 2008;37(2):298-311.
    • (2008) Mol Cell Neurosci , vol.37 , Issue.2 , pp. 298-311
    • Gumy, L.F.1    Bampton, E.T.2    Tolkovsky, A.M.3
  • 14
    • 36048968888 scopus 로고    scopus 로고
    • SOD2 protects neurons from injury in cell culture and animal models of diabetic neuropathy
    • 1:CAS:528:DC%2BD2sXhtlahurfP 17927981 2190625
    • Vincent AM, Russell JW, Sullivan KA, Backus C, Hayes JM, McLean LL, Feldman EL. SOD2 protects neurons from injury in cell culture and animal models of diabetic neuropathy. Exp Neurol. 2007;208(2):216-27.
    • (2007) Exp Neurol , vol.208 , Issue.2 , pp. 216-227
    • Vincent, A.M.1    Russell, J.W.2    Sullivan, K.A.3    Backus, C.4    Hayes, J.M.5    McLean, L.L.6    Feldman, E.L.7
  • 16
    • 33645100148 scopus 로고    scopus 로고
    • Axonal damage and demyelination in long-term dorsal root ganglia cultures from a rat model of Charcot-Marie-Tooth type 1A disease
    • 16553608
    • Nobbio L, Gherardi G, Vigo T, Passalacqua M, Melloni E, Abbruzzese M, Mancardi G, Nave KA, Schenone A. Axonal damage and demyelination in long-term dorsal root ganglia cultures from a rat model of Charcot-Marie-Tooth type 1A disease. Eur J Neurosci. 2006;23(6):1445-52.
    • (2006) Eur J Neurosci , vol.23 , Issue.6 , pp. 1445-1452
    • Nobbio, L.1    Gherardi, G.2    Vigo, T.3    Passalacqua, M.4    Melloni, E.5    Abbruzzese, M.6    Mancardi, G.7    Nave, K.A.8    Schenone, A.9
  • 17
    • 34347207265 scopus 로고    scopus 로고
    • Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity
    • 1:CAS:528:DC%2BD2sXhtFGntb3O 17401349
    • Malin SA, Davis BM, Molliver DC. Production of dissociated sensory neuron cultures and considerations for their use in studying neuronal function and plasticity. Nat Protoc. 2007;2(1):152-60.
    • (2007) Nat Protoc , vol.2 , Issue.1 , pp. 152-160
    • Malin, S.A.1    Davis, B.M.2    Molliver, D.C.3
  • 18
    • 84879482841 scopus 로고    scopus 로고
    • Identification of multisegmental nociceptive afferents that modulate locomotor circuits in the neonatal mouse spinal cord
    • 1:CAS:528:DC%2BC3sXpvFehsbc%3D 23436436
    • Mandadi S, Hong P, Tran MA, Bráz JM, Colarusso P, Basbaum AI, Whelan PJ. Identification of multisegmental nociceptive afferents that modulate locomotor circuits in the neonatal mouse spinal cord. J Comp Neurol. 2013;521(12):2870-87.
    • (2013) J Comp Neurol , vol.521 , Issue.12 , pp. 2870-2887
    • Mandadi, S.1    Hong, P.2    Tran, M.A.3    Bráz, J.M.4    Colarusso, P.5    Basbaum, A.I.6    Whelan, P.J.7
  • 19
    • 0034255439 scopus 로고    scopus 로고
    • Spared nerve injury: an animal model of persistent peripheral neuropathic pain
    • 1:STN:280:DC%2BD3cvosVSntw%3D%3D 10924808
    • Decosterd I, Woolf CJ. Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain. 2000;87(2):149-58.
    • (2000) Pain , vol.87 , Issue.2 , pp. 149-158
    • Decosterd, I.1    Woolf, C.J.2
  • 20
    • 0010945363 scopus 로고
    • Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells
    • 1:STN:280:DyaL3s7mvFOjtA%3D%3D 6188155 393426
    • Baccaglini PI, Hogan PG. Some rat sensory neurons in culture express characteristics of differentiated pain sensory cells. Proc Natl Acad Sci. 1983;80(2):594-8.
    • (1983) Proc Natl Acad Sci , vol.80 , Issue.2 , pp. 594-598
    • Baccaglini, P.I.1    Hogan, P.G.2
  • 21
    • 84930682495 scopus 로고    scopus 로고
    • Pannexin-1 up-regulation in the dorsal root ganglion contributes to neuropathic pain development
    • 1:CAS:528:DC%2BC2MXps1ems70%3D 25925949
    • Zhang Y, Laumet G, Chen SR, Hittelman WN, Pan HL. Pannexin-1 up-regulation in the dorsal root ganglion contributes to neuropathic pain development. J Biol Chem. 2015;290(23):14647-55.
    • (2015) J Biol Chem , vol.290 , Issue.23 , pp. 14647-14655
    • Zhang, Y.1    Laumet, G.2    Chen, S.R.3    Hittelman, W.N.4    Pan, H.L.5
  • 22
    • 84924970774 scopus 로고    scopus 로고
    • Moderate exercise training attenuates inflammatory mediators in DRG of Type 1 diabetic rats
    • 1:CAS:528:DC%2BC2MXks1agu74%3D 25783659
    • Yoon H, Thakur V, Isham D, Fayad M, Chattopadhyay M. Moderate exercise training attenuates inflammatory mediators in DRG of Type 1 diabetic rats. Exp Neurol. 2015;267:107-14.
    • (2015) Exp Neurol , vol.267 , pp. 107-114
    • Yoon, H.1    Thakur, V.2    Isham, D.3    Fayad, M.4    Chattopadhyay, M.5
  • 23
    • 2942746305 scopus 로고    scopus 로고
    • Isolation and culture of sensory neurons from the dorsal-root ganglia of embryonic or adult rats
    • 15131338
    • Burkey TH, Hingtgen CM, Vasko MR. Isolation and culture of sensory neurons from the dorsal-root ganglia of embryonic or adult rats. Methods Mol Med. 2004;99:189-202.
    • (2004) Methods Mol Med , vol.99 , pp. 189-202
    • Burkey, T.H.1    Hingtgen, C.M.2    Vasko, M.R.3
  • 24
    • 84859842597 scopus 로고    scopus 로고
    • Culture of dissociated sensory neurons from dorsal root ganglia of postnatal and adult rats
    • 1:CAS:528:DC%2BC2MXhslCns7g%3D 22367811
    • Owen DE, Egerton J. Culture of dissociated sensory neurons from dorsal root ganglia of postnatal and adult rats. Methods Mol Biol. 2012;846:179-87.
    • (2012) Methods Mol Biol , vol.846 , pp. 179-187
    • Owen, D.E.1    Egerton, J.2
  • 25
    • 84868295300 scopus 로고    scopus 로고
    • FQ: Genetic study of axon regeneration with cultured adult dorsal root ganglion neurons
    • 23117482
    • Saijilafu Zhou. FQ: Genetic study of axon regeneration with cultured adult dorsal root ganglion neurons. J Vis Exp. 2012;66:4141.
    • (2012) J Vis Exp , vol.66 , pp. 4141
    • Saijilafu, Z.1
  • 26
    • 84870233117 scopus 로고    scopus 로고
    • Sensory neuron downregulation of the Kv9.1 potassium channel subunit mediates neuropathic pain following nerve injury
    • 1:CAS:528:DC%2BC38XhslOqtLnM 23197740 3713313
    • Tsantoulas C, Zhu L, Shaifta Y, Grist J, Ward JP, Raouf R, Michael GJ, McMahon SB. Sensory neuron downregulation of the Kv9.1 potassium channel subunit mediates neuropathic pain following nerve injury. J Neurosci. 2012;32(48):17502-13.
    • (2012) J Neurosci , vol.32 , Issue.48 , pp. 17502-17513
    • Tsantoulas, C.1    Zhu, L.2    Shaifta, Y.3    Grist, J.4    Ward, J.P.5    Raouf, R.6    Michael, G.J.7    McMahon, S.B.8
  • 28
    • 84892981582 scopus 로고    scopus 로고
    • Chondrolectin affects cell survival and neuronal outgrowth in in vitro and in vivo models of spinal muscular atrophy
    • 1:CAS:528:DC%2BC2cXht1Ogt7o%3D 24067532
    • Sleigh JN, Barreiro-Iglesias A, Oliver PL, Biba A, Becker T, Davies KE, Becker CG, Talbot K. Chondrolectin affects cell survival and neuronal outgrowth in in vitro and in vivo models of spinal muscular atrophy. Hum Mol Genet. 2014;23(4):855-69.
    • (2014) Hum Mol Genet , vol.23 , Issue.4 , pp. 855-869
    • Sleigh, J.N.1    Barreiro-Iglesias, A.2    Oliver, P.L.3    Biba, A.4    Becker, T.5    Davies, K.E.6    Becker, C.G.7    Talbot, K.8
  • 29
    • 0018775912 scopus 로고
    • Development of mouse dorsal root ganglia: an autoradiographic and quantitative study
    • 1:STN:280:DyaL3c%2FivVOqug%3D%3D 490183
    • Lawson SN, Biscoe TJ. Development of mouse dorsal root ganglia: an autoradiographic and quantitative study. J Neurocytol. 1979;8(3):265-74.
    • (1979) J Neurocytol , vol.8 , Issue.3 , pp. 265-274
    • Lawson, S.N.1    Biscoe, T.J.2
  • 30
    • 73349096613 scopus 로고    scopus 로고
    • Dissection, plating, and maintenance of dorsal root ganglion neurons for monoculture and for coculture with dorsal horn neurons
    • Albuquerque C, Joseph DJ, Choudhury P, MacDermott AB. Dissection, plating, and maintenance of dorsal root ganglion neurons for monoculture and for coculture with dorsal horn neurons. Cold Spring Harb Protoc. 2009;8:5275.
    • (2009) Cold Spring Harb Protoc , vol.8 , pp. 5275
    • Albuquerque, C.1    Joseph, D.J.2    Choudhury, P.3    MacDermott, A.B.4
  • 31
    • 80355131697 scopus 로고    scopus 로고
    • The culture of primary motor and sensory neurons in defined media on electrospun poly-L-lactide nanofiber scaffolds
    • 21372783
    • Leach MK, Feng ZQ, Gertz CC, Tuck SJ, Regan TM, Naim Y, Vincent AM, Corey JM. The culture of primary motor and sensory neurons in defined media on electrospun poly-L-lactide nanofiber scaffolds. J Vis Exp. 2011;48:2389.
    • (2011) J Vis Exp , vol.48 , pp. 2389
    • Leach, M.K.1    Feng, Z.Q.2    Gertz, C.C.3    Tuck, S.J.4    Regan, T.M.5    Naim, Y.6    Vincent, A.M.7    Corey, J.M.8
  • 32
    • 84923561706 scopus 로고    scopus 로고
    • Dorsal root ganglia neurons and differentiated adipose-derived stem cells: an in vitro co-culture model to study peripheral nerve regeneration
    • de Luca AC, Faroni A, Reid AJ. Dorsal root ganglia neurons and differentiated adipose-derived stem cells: an in vitro co-culture model to study peripheral nerve regeneration. J Vis Exp. 2015;96:52543.
    • (2015) J Vis Exp , vol.96 , pp. 52543
    • De Luca, A.C.1    Faroni, A.2    Reid, A.J.3
  • 33
    • 79960652212 scopus 로고    scopus 로고
    • Rodent sensory neuron culture and analysis
    • Hall AK. Rodent sensory neuron culture and analysis. Curr Protoc Neurosci. 2006;36(3):1-13.
    • (2006) Curr Protoc Neurosci. , vol.36 , Issue.3 , pp. 1-13
    • Hall, A.K.1
  • 34
    • 84903372109 scopus 로고    scopus 로고
    • Morphological analysis of neuromuscular junction development and degeneration in rodent lumbrical muscles
    • 24530702 4120659
    • Sleigh JN, Burgess RW, Gillingwater TH, Cader MZ. Morphological analysis of neuromuscular junction development and degeneration in rodent lumbrical muscles. J Neurosci Methods. 2014;227:159-65.
    • (2014) J Neurosci Methods , vol.227 , pp. 159-165
    • Sleigh, J.N.1    Burgess, R.W.2    Gillingwater, T.H.3    Cader, M.Z.4
  • 35
    • 84896133061 scopus 로고    scopus 로고
    • Neuromuscular junction maturation defects precede impaired lower motor neuron connectivity in Charcot-Marie-Tooth type 2D mice
    • 1:CAS:528:DC%2BC2cXmsVKgsr8%3D 24368416 3990164
    • Sleigh JN, Grice SJ, Burgess RW, Talbot K, Cader MZ. Neuromuscular junction maturation defects precede impaired lower motor neuron connectivity in Charcot-Marie-Tooth type 2D mice. Hum Mol Genet. 2014;23(10):2639-50.
    • (2014) Hum Mol Genet , vol.23 , Issue.10 , pp. 2639-2650
    • Sleigh, J.N.1    Grice, S.J.2    Burgess, R.W.3    Talbot, K.4    Cader, M.Z.5
  • 36
    • 75149138783 scopus 로고    scopus 로고
    • Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos
    • 1:CAS:528:DC%2BC3cXks1GlsA%3D%3D 20057379
    • Wiese S, Herrmann T, Drepper C, Jablonka S, Funk N, Klausmeyer A, Rogers ML, Rush R, Sendtner M. Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos. Nat Protoc. 2010;5(1):31-8.
    • (2010) Nat Protoc , vol.5 , Issue.1 , pp. 31-38
    • Wiese, S.1    Herrmann, T.2    Drepper, C.3    Jablonka, S.4    Funk, N.5    Klausmeyer, A.6    Rogers, M.L.7    Rush, R.8    Sendtner, M.9
  • 37
    • 21344472483 scopus 로고    scopus 로고
    • A mutation in dynein rescues axonal transport defects and extends the life span of ALS mice
    • 1:CAS:528:DC%2BD2MXksFyqs7g%3D 15911875 2171702
    • Kieran D, Hafezparast M, Bohnert S, Dick JR, Martin J, Schiavo G, Fisher EM, Greensmith L. A mutation in dynein rescues axonal transport defects and extends the life span of ALS mice. J Cell Biol. 2005;169(4):561-7.
    • (2005) J Cell Biol , vol.169 , Issue.4 , pp. 561-567
    • Kieran, D.1    Hafezparast, M.2    Bohnert, S.3    Dick, J.R.4    Martin, J.5    Schiavo, G.6    Fisher, E.M.7    Greensmith, L.8


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.