Sensitivity improvement of a sandwich-type ELISA immunosensor for the detection of different prostate-specific antigen isoforms in human serum using electrochemical impedance spectroscopy and an ordered and hierarchically organized interfacial supramolecular architecture

Analytica Chimica Acta

Volumn 902, Issue , 2016, Pages 97-106

  Gutiérrez Zúñiga, Gabriela Guadalupe ^a   Hernández López, José Luis ^a  

^a CENTRO DE INVESTIGACIÓN Y DESARROLLO TECNOLÓGICO EN ELECTROQUÍMICA   (Mexico)

Author keywords

Electrochemical impedance spectroscopy; Enzyme linked immunosorbent assay; Immunosensor; Interfacial properties; Non specific protein adsorption; Prostate cancer

Indexed keywords

ANTIGENS; BODY FLUIDS; COENZYMES; DISEASES; ELECTROCHEMICAL ELECTRODES; ELECTRODES; ENZYMES; IMMUNOSENSORS; PROTEINS; SELF ASSEMBLED MONOLAYERS; SPECTROSCOPY; SUPRAMOLECULAR CHEMISTRY; UROLOGY;

ENZYME LINKED IMMUNOSORBENT ASSAY; FUNCTIONALIZED ELECTRODES; INTERFACIAL PROPERTY; NON-SPECIFIC PROTEIN ADSORPTION; POLARIZATION RESISTANCES; PROSTATE CANCERS; PROSTATE SPECIFIC ANTIGEN; SUPRAMOLECULAR ARCHITECTURES;

ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY;

PROSTATE SPECIFIC ANTIGEN; ISOPROTEIN;

ANALYTICAL PARAMETERS; ANTIGEN DETECTION; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; ELECTRODE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOSENSOR; MEXICAN; PRIORITY JOURNAL; SERUM; SUPRAMOLECULAR CHEMISTRY; BLOOD; CALIBRATION; DEVICES; GENETIC PROCEDURES; LIMIT OF DETECTION; PROCEDURES;

BIOSENSING TECHNIQUES; CALIBRATION; DIELECTRIC SPECTROSCOPY; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; LIMIT OF DETECTION; PROSTATE-SPECIFIC ANTIGEN; PROTEIN ISOFORMS;

EID: 84957056105     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2015.10.042     Document Type: Article

References (49)

1. Instituto Nacional de Cancerología. Available from: (accessed 14.04.15.). http://www.incan.salud.gob.mx.
2. SINAIS/SINAVE/DGE/SALUD Perfil epidemiológico de los tumores malignos en México 2011, Secretaría de Salud, México.
3. Steuber T., O'Brien M.F., Lilja H. Serum markers for prostate cancer: a rational approach to the literature. Eur. Urol. 2008, 54:31-40.
4. Cooner W.H., Mosley B.R., Rutherford C.L., Beard J.H., Pond H.S., Terry W.J., Igel T.C., Kidd D.D. Prostate cancer detection in a clinical urological practice by ultrasonography, digital rectal examination and prostate specific antigen. J. Urol. 1990, 143:1146-1152.
5. Schröder F.H., Hugosson J., Roobol M.J., et al. Screening and prostate-cancer mortality in a randomized European study. N. Engl. J. Med. 2009, 360:1320-1328.
6. Roddam A.W., Duffy M.J., Hamdy F.C., Ward A.M., Patnick J., Price C.P., Rimmer J., Sturgeon C., White P., Allen N.E. Use of prostate-specific antigen (PSA) isoforms for the detection of prostate cancer in men with a PSA level of 2--10 ng ml: systematic review and meta-analysis. Eur. Urol. 2005, 48:386-399.
7. Özen H., Sozen S. PSA isoforms in prostate cancer detection. Eur. Urol. 2006, 5:495-499.
8. Stenman U.H., Leinonen J., Zhang W.M., Finne P. Prostate-specific antigen, Semin. Cancer Biol. 1999, 9:83-93.
9. Catalona W.J., Bartsch G., Rittenhouse H.G., Evans C.L., Linton H.J., Amirkhan A., Horninger W., Klocker H., Mikolajczyk S.D. Serum pro prostate specific antigen improves cancer detection compared to free and complexed prostate specific antigen in men with prostate specific antigen 2 to 4 ng/ml. J. Urol. 2003, 170:2181-2185.
10. Linton H.J., Marks L.S., Millar L.S., Knott C.L., Rittenhouse H.G., Mikolajczyk S.D. Benign prostate-specific antigen (BPSA) in serum is increased in benign prostate disease. Clin. Chem. 2003, 49:253-259.
11. Mikolajczyk S.D., Song Y., Wong J.R., Matson R.S., Rittenhouse H.G. Are multiple markers the future of prostate cancer diagnostics?. Clin. Biochem. 2004, 37:519-528.
12. De Nunzio C., Kramer G., Marberger M., Montironi R., Nelson W., Schröder F., Sciarra A., Tubaro A. The controversial relationship between benign prostatic hyperplasia and prostate cancer: the role of inflammation. Eur. Urol. 2011, 60:106-117.
13. Fortin T., Salvador A., Charrier J.P., Lenz C., Lacoux X., Morla A., Choquet-Kastylevsky G., Lemoine J. Clinical quantitation of prostate-specific antigen biomarker in the low nanogram/millilitre range by conventional bore liquid chromatography-tandem mass spectrometry (multiple reaction monitoring) coupling and correlation with ELISA tests. Mol. Cell. Proteomics 2009, 8:1006-1015.
14. Healy D.A., Hayes C.J., Leonard P., McKenna L., O'Kennedy R. Biosensor developments: application to prostate-specific antigen detection. Trends Biotechnol. 2007, 25:125-131.
15. Krause S. Impedance methods. Encyclopedia of Electrochemistry Volume 3: Instrumentation and Electroanalytical Chemistry 2003, 196-229. Wiley-VCH, New Jersey. A.J. Bard, M. Stratmann, P.R. Unwin (Eds.).
16. Barsoukov E., MacDonald J.R. Impedance Spectroscopy: Theory, Experiments and Applications 2005, Wiley Interscience, New Jersey, (Chapter 1). second ed.
17. Guan J.-G., Miao Y.-Q., Zhang Q.-J. Impedimetric biosensors. J. Biosci. Bioeng. 2004, 97:219-226.
18. Yakovleva J., Emnéus J. Electrochemical immunoassays. Bioelectrochemistry. Fundamentals, Experimental Techniques and Applications 2008, 377-410. John Wiley and Sons, Ltd., The Atrium, Southern Gate, Chichester, West Sussex. P.N. Bartlett (Ed.).
19. Chen S., Liu L., Zhou J., Jiang S. Controlling antibody orientation on charged self-assembled monolayers. Langmuir 2003, 19:2859-2864.
20. Buyer's Guide to Biological Kits. Available from: (accessed 14.04.15.). http://www.biokits.com.
21. Romppanen J., Keskikuru R., Kataja V., Eskelinen M., Kosma V.-M., Savolainen K., Uusitupa M., Mononen I. Measurement of prostate-specific antigen in detection of benign or malignant breast disease in women. Br. J. Cancer 1999, 79:1583-1587.
22. Black M.H., Giai M., Ponzone R., Sismondi P., Yu H., Diamandis E.P. Serum total and free prostate-specific antigen for breast cancer diagnosis in women. Clin. Cancer Res. 2000, 6:467-473.
23. Sauter E.R., Klein G., Wagner-Mann C., Diamandis E.P. Prostate-specific antigen expression in nipple aspirate fluid is associated with breast cancer risk. Cancer Detect. Prev. 2004, 28:27-31.
24. Johnson E.D., Kotowski T.M. Detection of prostate-specific antigen by ELISA. J. Forensic Sci. 1993, 38:250-258.
25. Khaldi N., Miras A., Botti K., Benali L., Gromb S. Evaluation of three rapid detection methods for the forensic identification of seminal fluid in rape cases. J. Forensic Sci. 2004, 49:1-5.
26. Peonim V., Chirachariyavej T., Atamasirikul K., Talthip J. Comparable between rapid one step immunochromatographic assay and ELISA in the detection of prostate specific antigen in vaginal specimens of raped women. J. Med. Assoc. Thail. 2007, 90:2624-2629.
27. Tremiliosi-Filho G., Dall'Antonia L.H., Jerkiewicz G. Limit to extent of formation of the quasi-two-dimensional oxide state on Au electrodes. J. Electroanal. Chem. 1997, 422:149-159.
28. Angerstein-Kozlowska H., Conway B.E., Hamelin A., Stoicoviciu L. Elementary steps of electrochemical oxidation of single-crystal planes of Au-I. Chemical basis of processes involving geometry of anions and the electrode surfaces. Electrochim. Acta 1986, 31:1051-1061.
29. Hoare J.P. A cyclic voltammetric study of the gold-oxygen system. J. Electrochem. Soc. 1984, 131:1808-1815.
30. Oesch U., Janata J. Electrochemical study of gold electrodes with anodic oxide films-I. Formation and reduction behaviour of anodic oxides on gold. Electrochim. Acta 1983, 28:1237-1246.
31. Trasatti S., Petrii O.A. Real surface area measurements in electrochemistry. Pure Appl. Chem. 1991, 63:711-734.
32. Lin H.J., Kirsch J.F. A rapid, sensitive fluorometric assay for avidin and biotin. Methods in Enzymology 1979, vol. 62:287-289. Academic Press, Inc., New York.
33. Total- and Free- Prostate-Specific Antigen (t- and f-PSA) Test Systems. Available from: (accessed 14.04.15.). http://www.monobind.com/immunoassays-cancer%20markers-psa.
34. Orazem M.E., Tribollet B. Electrochemical Impedance Spectroscopy 2008, John Wiley and Sons, Inc., New Jersey.
35. O'Connell M.A., Belanger B.A., Haaland P.D. Calibration and assay development using the four-parameter logistic model. Chemom. Intell. Lab. Syst. 1993, 20:97-114.
36. Proll G., Tschmelak J., Gauglitz G. Fully automated biosensors for water analysis. Anal. Bioanal. Chem. 2005, 381:61-63.
37. Dudley R.A., Edwards P., Ekins R.P., Finney D.J., McKenzie I.G.M., Raab G.M., Rodbard D., Rodgers R.P.C. Guidelines for immunoassay data processing. Clin. Chem. 1985, 31:1264-1271.
38. Little J.A. Comparison of curve fitting models for ligand binding assays. Chromatographia 2004, 59:S177-S181.
39. Findlay J.W.A., Dillard R.F. Appropriate calibration curve fitting in ligand binding assays. AAPS J. 2007, 9:E260-E267.
40. Inczedy J., Lengyel T., Ure A.M. The Orange Book. Compendium of Analytical Nomenclature (Definitive Rules 1997) 1998, Blackwell Science, Oxford. third ed.
41. Bisquert J., Compte A. Theory of the electrochemical impedance of anomalous diffusion. J. Electroanal. Chem. 2001, 499:112-120.
42. Yang X., Zhang G. Simulation the structure and effect of the electrical double layer at nanometre electrodes. Nanotechnology 2007, 18:335201-335209.
43. Skoog D.A., Holler F.J., Crouch S.R. Principios de Análisis Instrumental, sexta ed 2008, Cengage Learning, Inc., México.
44. Motulsky H., Christopoulos A. Fitting Models to Biological Data Using Linear and Nonlinear Regression. A Practical Guide to Curve Fitting 2004, 47-57. Oxford University Press, Inc., New York.
45. Lind K., Kubista M. Development and evaluation of three real-time immuno-PCR assemblages for quantification of PSA. J. Immunol. Methods 2005, 304:107-116.
46. Fernandez-Sanchez C., McNeil C.J., Rawson K., Nilsson O. Disposable noncompetitive immunosensor for free and total prostate-specific antigen based on capacitance measurement. Anal. Chem. 2004, 76:5649-5656.
47. Fernandez-Sanchez C., Gallardo-Soto A.M., Rawson K., Nilsson O., McNeil C.J. Quantitative impedimetric immunosensor for free and total prostate specific antigen based on a lateral flow assay format. Electrochem. Commun. 2004, 6:138-143.
48. Sarkar P., Pal P.S., Ghosh D., Setford S.J., Tothill I.E. Amperometric biosensors for detection of the prostate cancer marker (PSA). Int. J. Pharm. 2002, 238:1-9.
49. Huang L., Reekmans G., Saerens D., Friedt J.M., Frederix F., Francis L., Muyldermans S., Campitelli A., Van Hoof C. Prostate-specific antigen immunosensing based on mixed self-assembled monolayers, camel antibodies and colloidal-gold enhanced sandwich assays. Biosens. Bioelectron. 2005, 21:483-490.