A conditional knockout toolkit for caenorhabditis elegans based on the Cre/loxP recombination

PLoS ONE

Volumn 9, Issue 12, 2014, Pages

  Kage Nakadai, Eriko ^a,b   Imae, Rieko ^a,c   Suehiro, Yuji ^a   Yoshina, Sawako ^a   Hori, Sayaka ^a   Mitani, Shohei ^a  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; PEPTIDES AND PROTEINS; PROTEIN LOXP; TRIMETHYLPSORALEN; UNCLASSIFIED DRUG; GREEN FLUORESCENT PROTEIN; INTEGRASE;

ARTICLE; CAENORHABDITIS ELEGANS; CONDITIONAL KNOCKOUT; CONTROLLED STUDY; DELETION MUTANT; EXPRESSION VECTOR; GENE INACTIVATION; GENETIC PROCEDURES; GENETIC RECOMBINATION; MICROBIAL GENE; MUSCLE TWITCH; NONHUMAN; PROMOTER REGION; RNA INTERFERENCE; SID 1 GENE; SINGLE COPY INTEGRATION; TISSUE SPECIFICITY; TRANSGENIC ORGANISM; ULTRAVIOLET RADIATION; VALIDATION STUDY; ANIMAL; ANTIBODY SPECIFICITY; BIOSYNTHESIS; GENE VECTOR; GENETIC ENGINEERING; GENETICS; PROCEDURES; TRANSGENIC ANIMAL;

CAENORHABDITIS ELEGANS;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CAENORHABDITIS ELEGANS; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INTEGRASES; ORGAN SPECIFICITY; RNA INTERFERENCE;

EID: 84956618244     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0114680     Document Type: Article

References (24)

1. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, et al. (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474: 337-342.
2. Mello C, Fire A (1995) DNA Transformation.
3. Kamath RS, Martinez-Campos M, Zipperlen P, Fraser AG, Ahringer J (2001) Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol 2: RESEARCH0002.
4. Cheng Z, Yi P, Wang X, Chai Y, Feng G, et al. (2013) Conditional targeted genome editing using somatically expressed TALENs in C. Elegans. Nat Biotechnol 31: 934-937.
5. Liu P, Long L, Xiong K, Yu B, Chang N, et al. (2014) Heritable/conditional genome editing in C. Elegans using a CRISPR-Cas9 feeding system. Cell Res 24: 886-889.
6. Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, et al. (2014) Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. Elegans Neural Development. Dev Cell.
7. Consortium CeDM (2012) large-scale screening for targeted knockouts in the Caenorhabditis elegans genome. G3 (Bethesda) 2: 1415-1425.
8. Frøkjaer-Jensen C, Davis MW, Hopkins CE, Newman BJ, Thummel JM, et al. (2008) Single-copy insertion of transgenes in Caenorhabditis elegans. Nat Genet 40: 1375-1383.
9. Kage-Nakadai E, Kobuna H, Funatsu O, Otori M, Gengyo-Ando K, et al. (2012) Single/low-copy integration of transgenes in Caenorhabditis elegans using an ultraviolet trimethylpsoralen method. BMC Biotechnol 12: 1.
10. Kage-Nakadai E, Imae R, Yoshina S, Mitani S (2014) Methods for single/low-copy integration by ultraviolet and trimethylpsoralen treatment in Caenorhabditis elegans. Methods 68: 397-402.
11. Austin S, Ziese M, Sternberg N (1981) A novel role for site-specific recombination in maintenance of bacterial replicons. Cell 25: 729-736.
12. Broach JR, Guarascio VR, Jayaram M (1982) Recombination within the yeast plasmid 2mu circle is site-specific. Cell 29: 227-234.
13. Gu H, Marth JD, Orban PC, Mossmann H, Rajewsky K (1994) Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265: 103-106.
14. Buchholz F, Ringrose L, Angrand PO, Rossi F, Stewart AF (1996) Different thermostabilities of FLP and Cre recombinases: implications for applied site-specific recombination. Nucleic Acids Res 24: 4256-4262.
15. Davis MW, Morton JJ, Carroll D, Jorgensen EM (2008) Gene activation using FLP recombinase in C. Elegans. PLoS Genet 4: e1000028.
16. Voutev R, Hubbard EJ (2008) A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. Genetics 180: 103-119.
17. Hubbard EJ (2014) FLP/FRT and Cre/lox recombination technology in C. Elegans. Methods 68: 417-424.
18. Hoier EF, Mohler WA, Kim SK, Hajnal A (2000) The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression. Genes Dev 14: 874-886.
19. Flavell SW, Pokala N, Macosko EZ, Albrecht DR, Larsch J, et al. (2013) Serotonin and the neuropeptide PDF initiate and extend opposing behavioral states in C. Elegans. Cell 154: 1023-1035.
20. Brenner S (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71-94.
21. Gengyo-Ando K, Mitani S (2000) Characterization of mutations induced by ethyl methanesulfonate, UV, and trimethylpsoralen in the nematode Caenorhabditis elegans. Biochem Biophys Res Commun 269: 64-69.
22. Gengyo-Ando K, Yoshina S, Inoue H, Mitani S (2006) An efficient transgenic system by TA cloning vectors and RNAi for C. Elegans. Biochem Biophys Res Commun 349: 1345-1350.
23. Mitani S (1995) Gnetic regulation of mec-3 gene expression implicated in the specification of the mechanosensory neuron cell types in Caenorhabditis elegans. Develop Growth Differ 37: 551-557.
24. Macosko EZ, Pokala N, Feinberg EH, Chalasani SH, Butcher RA, et al. (2009) A hub-and-spoke circuit drives pheromone attraction and social behaviour in C. Elegans. Nature 458: 1171-1175.