Biotransformation as a preparative method for the synthesis of optically active silanes, germanes, and digermanes: Studies on the (R)-selective microbial reduction of MePh(Me3C)ElC(O)Me (El = Si, Ge), MePh(Me3Ge)GeC(O)Me, and MePh(Me3Si)GeC(O)Me using resting cells of Saccharomyces cerevisiae (DHW S-3) as biocatalyst

Organosilicon Chemistry II: From Molecules to Materials

Volumn , Issue , 1996, Pages 237-242

  Wagner, Stephan A ^a   Brakmann, Susanne ^a   Tacke, Reinhold ^a  

^a UNIVERSITY OF KARLSRUHE   (Germany)

Author keywords

Biocatalyst; Biotransformations; Carbon atoms; Microbial reductions; Racemic compounds

Indexed keywords

ATOMS; BIOCATALYSTS; BIOCONVERSION; CARBON; MIXTURES; SILANES; SILICON CARBIDE; YEAST;

BIOTRANSFORMATIONS; CARBON ATOMS; ENANTIOMERIC PURITY; MICROBIAL REDUCTION; OPTICALLY ACTIVE; RACEMIC COMPOUNDS; REDUCTION PRODUCTS; YEAST SACCHAROMYCES CEREVISIAE;

SI-GE ALLOYS;

EID: 84955723695     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9783527619894.ch32     Document Type: Chapter

References (6)

1. R. Tacke, S. Brakmann, F. Wuttke, J. Fooladi, C. Syldatk, D. Schomburg, J. Organomet. Chem. 1991, 403,29; and references cited therein.
2. R. Tacke, S. A. Wagner, J. Sperlich, Chem. Ber. 1994,127,639; and references cited therein.
3. L. Fischer, S. A. Wagner, R. Tacke, Appl. Microbiol. Biotechnol. 1995,42,671.
4. R. Tacke, K. Fritsche, A. Tafel, F. Wuttke, J. Organomet. Chem. 1990,388,47.
5. 4, 30.0 g/L glucose, 50 g/L washed yeast cells). The bioconversion products were isolated by extraction of the culture broths with ethyl acetate, followed by chromatographic purification (silica gel) and Kugelrohr distillation. For monitoring of the bioconversions, extracts of the culture broths were analyzed by gas chromatography.
6. 1H NMR spectroscopy, after derivatization with (R)-2-methoxy-2-(trifuoromethyl)-2-phenylacetyl chloride. These derivatives were also used to determine the absolute configurations at the carbon atoms of the respective El-CH(0H)Me (El = Si, Ge) moieties, following the strategy described in [3].