The glycol methacrylate embedding resins-Technovit 7100 and 8100

Plant Microtechniques and Protocols

Volumn , Issue , 2015, Pages 67-82

  Yeung, Edward C ^a   Chan, Colin K W ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84955419914     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/978-3-319-19944-3_4     Document Type: Chapter

References (25)

1. Newman SB, Borysko E, Swerdlow M (1949) New sectioning techniques for light and electron microscopy. Science 110:66-68
2. Litwin JA (1985) Light microscopic histochemistry on plastic sections. Prog Histochem Cytochem 16:1-84
3. Rosenberg M, Bartl P, Lesko J (1960) Water-soluble methacrylate as an embedding medium for the preparation of ultrathin sections. J Ultrast Res 4:298-303
4. Feder N, O'Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123-142
5. Yeung EC, Chan CKW (2015) Glycol methacrylate: the art of embedding and serial sectioning. Botany 93:1-8
6. Bennett HS, Wyrick AD, Lee SW, McNiel HJ Jr (1976) Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains. Stain Technol 51:71-97
7. Gerrits PO, Smid L (1983) A new, less toxic polymerization system for the embedding of soft tissues in glycol methacrylate and subsequent preparing of serial sections. J Microscopy 132:81-85
8. Gerrits PO, Horobin RW (1996) Glycol methacrylate embedding for light microscopy: basic principles and trouble-shooting. J Histotech 19:297-311
9. Lackie S, Yeung EC (1996) Zygotic embryo development in Daucus carota. Can J Bot 74:990-998
10. Glauert AM, Lewis PR (1998) Biological specimen preparation for transmission electron microscopy. Princeton University press, New Jersey
11. Lee Y-I, Hsu S-T, Yeung EC (2013) Orchid protocorm-like bodies are somatic embryos. Amer J Bot 100:2121-2131
12. O'Brien TP, McCully ME (1981) The study of plant structure: principles and selected methods. Termarcarphi, Melbourne
13. Ruzin SE (1999) Plant microtechnique and microscopy. Oxford University Press, New York
14. Horobin RW (1982) Histochemistry. Gustav Fischer, Stuttgart
15. Reid N (1975) Ultramicrotomy. North-Holland Publishing Co., Amsterdam
16. Hand NM (2002) Plastic embedding media and techniques. In: Bancroft JD, Gamble M (eds) Theory and practice of histological techniques. Churchill Livingstone, London, pp 663-677
17. Quester R, Knifka J, Schroder R (2002) Optimization of glycol methacrylate embedding of large specimens in neurological research. Study of rat skull-brain specimens after implantation of polyester meshes. J Neurosci Meth 113:15-26
18. Yeung EC, Law SK (1987) Serial sectioning techniques for a modified LKB Historesin. Stain Technol 62:147-153
19. Yeung EC (1984) Histological and histochemical staining methods. In: Vasal IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic, New York, pp 689-697
20. Yeung EC, Saxena PK (2005) Histological techniques. In: Jain SM, Gupta PK (eds) Protocols for somatic embryogenesis in woody plants. Springer, Dordrecht, pp 517-538
21. Soukup A (2014) Selected simple methods of plant cell wall histochemistry and staining for light microscopy. Meth Mol Biol 1080:25-52
22. Pappas PW (1971) The use of a chrome alum-gelatin (subbing) solution as a general adhesive for paraffin sections. Stain Technol 46:121-124
23. Gerrits PO, Eppinger B (1995) Effects of storing initiated 2-hydroxyethyl methacrylate solutions for embedding tissues for light microscopy: some practical implications. Biotech Histochem 70:155-163
24. Goodbody KC, Lloyd CW (1994) Immunofluorescence techniques for analysis of the cytoskeleton. In: Harris N, Oparka KJ (eds) Plant cell biology. IRL Press, Oxford, pp 221-243
25. Gerrits PO, Horobin RW, Stokroos I (1992) The effects of glycol methacrylate as a dehydrating agent on the dimensional changes of liver tissue. J Microscopy 165:273-280