A multiplexed method for kinetic measurements of apoptosis and proliferation using live-content imaging

Methods in Molecular Biology

Volumn 1219, Issue , 2015, Pages 35-42

  Artymovich, Katherine ^a   Appledorn, Daniel M ^a  

^a NONE

Author keywords

Caspase 3; Fluorescent imaging; Kinetic multiplex cell death assay; Real time imaging

Indexed keywords

ANALYTIC METHOD; APOPTOSIS; AREA UNDER THE CURVE; ARTICLE; CANCER CELL; CELL PROLIFERATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMAGE PROCESSING; IN VITRO STUDY; INCUCYTE ZOOM; PRIORITY JOURNAL; REGULATORY MECHANISM; TISSUE CULTURE; ANIMAL; CELL LINE; CULTURE TECHNIQUE; DEVICES; HELA CELL LINE; KINETICS; MOLECULAR IMAGING; PROCEDURES;

CASPASE 3; CASPASE 7; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS;

ANIMALS; APOPTOSIS; CASPASE 3; CASPASE 7; CELL CULTURE TECHNIQUES; CELL LINE; CELL PROLIFERATION; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; INDICATORS AND REAGENTS; KINETICS; MOLECULAR IMAGING;

EID: 84955328490     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-1661-0_4     Document Type: Article

References (3)

1. Hanahan D, Weinberg RA (2011) Hallmarks of cancer: the next generation. Cell 144:646–674
2. Vega-Avila E, Pugsley MK (2011) An overview of colorimetric assay methods used to assess survival or proliferation of mammalian cells. Proc West Pharmacol Soc 54:10–14
3. Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L (2006) Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fl uorescent indicator probe. Cytometry A 69:477–486