CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology

ACS Synthetic Biology

Volumn 5, Issue 1, 2016, Pages 99-103

  Iverson, Sonya V ^a   Haddock, Traci L ^a   Beal, Jacob ^c   Densmore, Douglas M ^a,b  

^a BOSTON UNIVERSITY   (United States)

^b Boston University   (United States)

^c BBN TECHNOLOGIES   (United States)

Author keywords

assembly; modular; multiplex; part library; Type IIS

Indexed keywords

ARTICLE; BINDING SITE; CHEMICAL REACTION; COMBINATORIAL LIBRARY; CONTROLLED STUDY; ESCHERICHIA COLI; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; REACTION TIME; RIBOSOME; SYNTHETIC BIOLOGY; COMBINATORIAL CHEMISTRY; GENE LIBRARY; GENETICS; METABOLISM; PROCEDURES;

FLUORESCEIN;

CLONING, MOLECULAR; COMBINATORIAL CHEMISTRY TECHNIQUES; ESCHERICHIA COLI; FLUORESCEIN; GENE LIBRARY; SYNTHETIC BIOLOGY;

EID: 84954443268     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.5b00124     Document Type: Article

References (13)

1. Lee, M. E., DeLoache, W. C., Cervantes, B., and Dueber, J. E. (2015) A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly ACS Synth Biol. 4, 975-986 10.1021/sb500366v
2. Weber, E., Engler, C., Gruetzner, R., Werner, S., and Marillonnet, S. (2011) A modular cloning system for standardized assembly of multigene constructs PLoS One 6, e16765 10.1371/journal.pone.0016765
3. Engler, C., Gruetzner, R., Kandzia, R., and Marillonnet, S. (2009) Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes PLoS One 4, e5553 10.1371/journal.pone.0005553
4. Engler, C., Youles, M., Gruetzner, R., Ehnert, T. M., Werner, S., Jones, J. D., Patron, N. J., and Marillonnet, S. (2014) A Golden Gate Modular Cloning Toolbox for Plants ACS Synth Biol. 3, 839-843 10.1021/sb4001504
5. Agmon, N., Mitchell, L. A., Cai, Y., Ikushima, S., Chuang, J., Zheng, A., Choi, W. J., Martin, J. A., Caravelli, K., Stracquadanio, G., and Boeke, J. D. (2015) Yeast Golden Gate (yGG) for efficient assembly of S. cerevisiae transcription units ACS Synth Biol. 4, 853-859 10.1021/sb500372z
6. Storch, M., Casini, A., Mackrow, B., Fleming, T., Trewhitt, H., Ellis, T., and Baldwin, G. S. (2015) BASIC: a new Biopart Assembly Standard for Idempotent Cloning provides accurate, single-tier DNA assembly for synthetic biology ACS Synth Biol. 4, 781-787 10.1021/sb500356d
7. Mutalik, V. K., Guimaraes, J. C., Cambray, G., Lam, C., Christoffersen, M. J., Mai, Q. A., Tran, A. B., Paull, M., Keasling, J. D., Arkin, A. P., and Endy, D. (2013) Precise and reliable gene expression via standard transcription and translation initiation elements Nat. Methods 10, 354-360 10.1038/nmeth.2404
8. Beal, J., Weiss, R., Densmore, D., Adler, A., Appleton, E., Babb, J., Bhatia, S., Davidsohn, N., Haddock, T., and Loyall, J. (2012) An End-to-End Workflow for Engineering of Biological Networks from High-Level Specifications ACS Synthetic Biology. 1, 317-331 10.1021/sb300030d
9. Ham, T. S., Dmytriv, Z., Plahar, H., Chen, J., Hillson, N. J., and Keasling, J. D. (2012) Design, implementation and practice of JBEI-ICE: an open source biological part registry platform and tools Nucleic Acids Res. 40, e141 10.1093/nar/gks531
10. TM Technical Note, Spherotech, Inc., Lake Forest, IL.
11. Appleton, E., Tao, J., Haddock, T., and Densmore, D. (2014) Interactive assembly algorithms for molecular cloning Nat. Methods 11, 657-662 10.1038/nmeth.2939
12. Bhatia, S. and Densmore, D. (2013) Pigeon: A Design Visualizer for Synthetic Biology ACS Synth Biol. 2, 348-350 10.1021/sb400024s
13. Beal, J., Weiss, R., Densmore, D., Adler, A., Appleton, E., Babb, J., Bhatia, S., Davidsohn, N., Haddock, T., Loyall, J., Schantz, R., Vasilev, V., and Yaman, F. (2012) An end-to-end workflow for engineering of biological networks from high-level specifications ACS Synth. Biol. 1, 317-331 10.1021/sb300030d