In Situ Hybridization to Embryo Whole Mounts and Tissue Sections: m RNA Detection and Application to Developmental Studies

In Situ Hybridization: Laboratory Companion

Volumn , Issue , 2008, Pages 91-121

  Jowett, T ^a   Mancera, M ^a   Amores, A ^a   Yan, Y L ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Dimethylformamide; DPX mountant; Immunohistochemical; Visualisation; Xenopus and mouse

Indexed keywords

VISUALIZATION;

DPX MOUNTANT; IMMUNOHISTOCHEMICAL; IN-SITU HYBRIDIZATION; RNA DETECTION; TISSUE SECTIONS; XENOPUS;

DIMETHYLFORMAMIDE;

EID: 84953408590     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9783527615070.ch5     Document Type: Chapter

References (12)

1. Forbes, Z. and Ingham, R W. (1993) Drosophila embryos. In "Essential Developmental Biology: A Practical Approach" pp 1-13. Ed. CD. Stern and P.W. Holland. Publ. IRL Press, Oxford.
2. Harland, R. M. (1991) In situ hybridization: an improved whole mount method for Xenopus embryos. In Methods in Cell Biology 36: 685-695.
3. Hauptmann, G. and Gerster, T. (1994) Trends in Genetics 10: 266. Two-color whole-mount in situ hybridization to vertebrate and Drosophilai embryos.
4. Henrique, D., Adam, J., Myat, A., Chitnis, A., Lewis, J. and Ish-Horowicz, D. (1995) Nature 375: 787-790. Expression of a Delta homologue in prospective neurons in the chick.
5. Jowett, T. and Lettice, L. (1994) Trends in Genetics 10: 73-74. Whole-mount in situ hybridization on zebrafish embryos using a mixture of digoxigenin and fluorescein-labelled probes.
6. Krauss, S., Johansen, T., Korzh, V. and Fjose, A. (1991) Development 113: 1193-1206. Expression of the zebrafish paired box gene pax during early neurogenesis.
7. Lamb, T. M., Knecht, A. K., Smith, W. C, Stachel, S. E., Economides, A. N., Stahl, N., Yancopolous, G. D. and Harland, R. M. (1993) Science 262: 713-718. Neural induction by the secreted polypeptide Noggin.
8. Oxtoby, E. and Jowett, T. (1993) Nucl. Acids Res. 21: 1087-1095. Cloning of the zebrafish krox-20 gene (krx-20) and its expression during hindbrain development.
9. Strähle, U., Blader, P., Adam, J. and Ingham, P. W. (1994) Trends in Genetics 10: 75-76. A simple and efficient procedure for non-isotopic in situ hybridization to sectioned material.
10. Tautz, D., Hülskamp, M. and Sommer R.J. (1992) Whole mount in situ hybridization in Drosophila. In "In situ hybridization: A Practical Approach" pp 61-73. Ed. D.G Wilkinson Publ. IRL Press, Oxford.
11. Wilkinson, D.G. (1992) The Theory and Practice of In situ Hybridization. In "In situ hybridization: A Practical Approach" pp 1-13. Ed. D.G. Wilkinson Publ. IRL Press, Oxford.
12. Yan, Y.-L, Hatto, K., Riggleman, B. and Postlethwait, J. H. (1995) Developmental Dynamics 203: 363-376. Expression of a type II collagen gene in the zebrafish embryonic axis.