High sensitivity detection of HIV-1 using two genomic targets compared with single target PCR

Journal of Medical Virology

Volumn 88, Issue 6, 2016, Pages 1092-1097

  Shah, Krishna ^a,b   Ragupathy, Viswanath ^a   Saga, Anusha ^c   Hewlett, Indira ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b Northwest High School   (United States)

^c Thomas Jefferson High School for Science and Technology   (United States)

Author keywords

Dual target; False negative; HIV NAT assay; Primer probe mis match; Sensitivity

Indexed keywords

CAMEROON; CHEMICAL BINDING; CLINICAL ARTICLE; CONTROLLED STUDY; FEASIBILITY STUDY; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN TISSUE; LONG TERMINAL REPEAT; PLASMA; VIRUS LOAD; ADULT; BLOOD; FEMALE; GENETIC VARIATION; GENETICS; HIV INFECTIONS; ISOLATION AND PURIFICATION; MALE; MUTATION; POLYMERASE CHAIN REACTION; PROCEDURES; SENSITIVITY AND SPECIFICITY; VIROLOGY; VIRUS GENOME;

PRIMER DNA; VIRUS RNA;

ADULT; CAMEROON; DNA PRIMERS; FEMALE; GENETIC VARIATION; GENOME, VIRAL; HIV INFECTIONS; HIV-1; HUMANS; MALE; MUTATION; POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

EID: 84951008288     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.24431     Document Type: Article

References (19)

1. Buonaguro L, Tornesello ML, Buonaguro FM. 2007. Human immunodeficiency virus type 1 subtype distribution in the worldwide epidemic: Pathogenetic and therapeutic implications. J Virol 81:10209-10219.
2. Centers for Disease Control and Prevention and Association of Public Health Laboratories. Laboratory Testing for the Diagnosis of HIV Infection: Updated Recommendations. Available at http://stacks.cdc.gov/view/cdc/23447. Published June 27, 2014.
3. Chudy M, Kress J, Halbauer J, Heiden M, Funk MB, Nubling CM. 2014. Risk minimization measures for blood screening HIV-1 Nucleic Acid Amplification Technique Assays in Germany. Transfus Med Hemother 41:45-51.
4. Coste J, Montes B, Reynes J, Peeters M, Segarra C, Delaporte E, Segondy M. 1997. Effect of HIV-1 genetic diversity on HIV-1 RNA quantification in plasma: Comparative evaluation of three commercial assays. J Acquir Immune Defic Syndr Hum Retrovirol 15:174-175.
5. Delaugerre C, Denis B, Peytavin G, Palmer P, Mourez T, Le Goff J, Molina JM, Simon F. 2009. Clinical and resistance consequences of misquantification of plasma and cerebrospinal fluid human immunodeficiency virus type 1 (HIV-1) RNA in samples from an HIV-1 subtype G-infected patient. J Clin Microbiol 47:3763-3764.
6. Edelmann A, Kalus U, Oltmann A, Stein A, Unbehaun A, Drosten C, Kruger DH, Hofmann J. 2010. Improvement of an ultrasensitive human immunodeficiency virus type 1 real-time reverse transcriptase-polymerase chain reaction targeting the long terminal repeat region. Transfusion 50:685-692.
7. Fearon M. 2005. The laboratory diagnosis of HIV infections. Can J Infect Dis Med Microbiol 16:26-30.
8. Foglieni B, Candotti D, Guarnori I, Raffaele L, Berzuini A, Spreafico M, Orani A, Rossotti R, Rossi D, Allain JP, Prati D. 2011. A cluster of human immunodeficiency virus Type 1 recombinant form escaping detection by commercial genomic amplification assays. Transfusion 51:719-730.
9. HIV sequence database: http://www.hiv.lanl.gov/content/sequence/HIV/MAP/landmark.html.
10. Korn K, Weissbrich B, Henke-Gendo C, Heim A, Jauer CM, Taylor N, Eberle J. 2009. Single-point mutations causing more than 100-fold underestimation of human immunodeficiency virus type 1 (HIV-1) load with the Cobas TaqMan HIV-1 real-time PCR assay. J Clin Microbiol 47:1238-1240.
11. Kwok S, Kellogg DE, McKinney N, Spasic D, Goda L, Levenson C, Sninsky JJ. 1990. Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies. Nucleic Acids Res 18:999-1005.
12. Lihana RW, Ssemwanga D, Abimiku A, Ndembi N. 2012. Update on HIV-1 diversity in Africa: a decade in review. AIDS Rev 14:83-100.
13. Muller B, Nubling CM, Kress J, Roth WK, De Zolt S, Pichl L. 2013. How safe is safe: New human immunodeficiency virus Type 1 variants missed by nucleic acid testing. Transfusion 53:2422-2430.
14. Praparattanapan J, Tragoolpua Y, Pathom-aree W, Kotarathitithum W, Chaiwarith R, Nuntachit N, Sirisanthana T, Supparatpinyo K. 2011. Current molecular epidemiology and recombination of HIV type 1 subtypes in northern Thailand. AIDS Res Hum Retroviruses 27:1201-1206.
15. Roth WK, Buhr S, Drosten C, Seifried E. 2000. NAT and viral safety in blood transfusion. Vox Sang 78:257-259.
16. Santoro MM, Perno CF. 2013. HIV-1 genetic variability and clinical implications. ISRN microbiology 2013:481314.
17. Schmidt M, Korn K, Nubling CM, Chudy M, Kress J, Horst HA, Geusendam G, Hennig H, Sireis W, Rabenau HF, Doerr HW, Berger A, Hourfar MK, Gubbe K, Karl A, Fickenscher H, Tischer BK, Babiel R, Seifried E, Gurtler L. 2009. First transmission of human immunodeficiency virus Type 1 by a cellular blood product after mandatory nucleic acid screening in Germany. Transfusion 49:1836-1844.
18. Stekler J, Maenza J, Stevens CE, Swenson PD, Coombs RW, Wood RW, Campbell MS, Nickle DC, Collier AC, Golden MR. 2007. Screening for acute HIV infection: lessons learned. Clin Infect Dis 44:459-461.
19. Van Laethem K, Beuselinck K, Van Dooren S, De Clercq E, Desmyter J, Vandamme AM. 1998. Diagnosis of human immunodeficiency virus infection by a polymerase chain reaction assay evaluated in patients harbouring strains of diverse geographical origin. J Virol Methods 70:153-166.