A non-invasive miRNA based assay to detect bladder cancer in cell-free urine

American Journal of Translational Research

Volumn 7, Issue 11, 2015, Pages 2500-2509

  De Long, Jessica ^a   Sullivan, Travis B ^a   Humphrey, John ^a   Logvinenko, Tanya ^b,c   Summerhayes, Kelly A ^a   Kozinn, Spencer ^a   Harty, Niall ^a   Summerhayes, Ian C ^a   Libertino, John A ^a   Holway, Antonia H ^a   Rieger Christ, Kimberly M ^a  

^a LAHEY HOSPITAL AND MEDICAL CENTER   (United States)

^b TUFTS MEDICAL CENTER   (United States)

^c BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Cell free system; Exosomes; MicroRNA; Urinary bladder neoplasm; Urine

Indexed keywords

MICRORNA; MICRORNA 191; MICRORNA 26A; MICRORNA 93; MICRORNA 940; UNCLASSIFIED DRUG;

ADULT; AGED; ARTICLE; BLADDER CANCER; CONTROLLED STUDY; DISEASE COURSE; FEMALE; GENE EXPRESSION PROFILING; HUMAN; MAJOR CLINICAL STUDY; MALE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING;

EID: 84950126245     PISSN: None     EISSN: 19438141     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. National Cancer Institute. Available online: http://www.cancer.gov/cancertopics/types/ bladder (accessed 03 June 2015).
2. Wu XR. Urothelial tumorigenesis: a tale of divergent pathways. Nat Rev Cancer 2005; 5: 713-25.
3. Farazi TA, Hoell JI, Morozov P, Tuschl T. MicroRNAs in human cancer. Adv Exp Med Biol 2013; 774: 1-20.
4. Li Y, Xu Z, Wang K, Wang N, Zhu M. Network analysis of microRNAs, genes and their regulation in human bladder cancer. Biomed Rep 2013; 1: 918-924.
5. Wszolek MF, Rieger-Christ KM, Kenney PA, Gould JJ, Silva Neto B, Lavoie AK, Logvinenko T, Libertino JA, Summerhayes IC. A MicroRNA expression profile defining the invasive bladder tumor phenotype. Urol Oncol 2011; 29: 794-801, e1.
6. Mlcochova H, Hezova R, Stanik M, Slaby O. Urine microRNAs as potential noninvasive biomarkers in urologic cancers. Urol Oncol 2014; 32: 41, e1-9.
7. Shi Z, Wei Q, Zhang M, She J. MicroRNAs in bladder cancer: expression profiles, biological functions, regulation, and clinical implications. Crit Rev Eukaryot Gene Expr 2014; 24: 55-75.
8. Hanke M, Hoefig K, Merz H, Feller AC, Kausch I, Jocham D, Warnecke JM, Sczakiel G. A robust methodology to study urine microRNA as tumor marker: microRNA-126 and microRNA-182 are related to urinary bladder cancer. Urol Oncol 2010; 28: 655-661.
9. Miah S, Dudziec E, Drayton RM, Zlotta AR, Morgan SL, Rosario DJ, Hamdy FC, Catto JW. An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. Br J Cancer 2012; 107: 123-128.
10. Mengual L, Lozano JJ, Ingelmo-Torres M, Gazquez C, Ribal MJ, Alcaraz A. Using microRNA profiling in urine samples to develop a noninvasive test for bladder cancer. Int J Cancer 2013; 133: 2631-2641.
11. Shimizu T, Suzuki H, Nojima M, Kitamura H, Yamamoto E, Maruyama R, Ashida M, Hatahira T, Kai M, Masumori N, Tokino T, Imai K, Tsukamoto T, Toyota M. Methylation of a panel of microRNA genes is a novel biomarker for detection of bladder cancer. Eur Urol 2013; 63: 1091-1100.
12. Wang G, Chan ES, Kwan BC, Li PK, Yip SK, Szeto CC, Ng CF. Expression of microRNAs in the urine of patients with bladder cancer. Clin Genitourin Cancer 2012; 10; 106-113.
13. Kim SM, Kang HW, Kim WT, Kim YJ, Yun SJ, Lee SC, Kim WJ. Cell-Free microRNA-214 From Urine as a Biomarker for Non-Muscle-Invasive Bladder Cancer. Korean J Urol 2013; 54: 791-796.
14. Yun SJ, Jeong P, Kim WT, Kim TH, Lee YS, Song PH, Choi YH, Kim IY, Moon SK, Kim WJ. Cellfree microRNAs in urine as diagnostic and prognostic biomarkers of bladder cancer. Int J Oncol 2012; 41: 1871-1878.
15. Inal JM, Fairbrother U, Heugh S. Microvesiculation and disease. Biochem Soc Trans 2013; 41: 237-240.
16. Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 2013; 200: 373-383.
17. Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ, Lötvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007; 9: 654-659.
18. Huang X, Liang M, Dittmar R, Wang L. Extracellular microRNAs in urologic malignancies: chances and challenges. Int J Mol Sci 2013; 14: 14785-14799.
19. Zhao L, Liu W, Xiao J, Cao B. The role of exosomes and “exosomal shuttle microRNA” in tumorigenesis and drug resistance. Cancer Lett 2015; 356: 339-346.
20. Pisitkun T, Shen RF, Knepper MA. Identification and proteomic profiling of exosomes in human urine. Proc Natl Acad Sci U S A 2004; 101, 13368-13373.
21. Chen CL, Lai YF, Tang P, Chien KY, Yu JS, Tsai CH, Chen HW, Wu CC, Chung T, Hsu CW, Chen CD, Chang YS, Chang PL, Chen YT. Comparative and targeted proteomic analyses of urinary microparticles from bladder cancer and hernia patients. J Proteome Res 2012; 11: 5611-5629.
22. Principe S, Jones EE, Kim Y, Sinha A, Nyalwidhe JO, Brooks J, Semmes OJ, Troyer DA, Lance RS, Kislinger T, Drake RR. In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine. Proteomics 2013; 13: 1667-1671.
23. Dijkstra S, Birker IL, Smit FP, Leyten GH, de Reijke TM, van Oort IM, Mulders PF, Jannink SA, Schalken JA. Prostate cancer biomarker profiles in urinary sediments and exosomes. J Urol 2014; 191: 1132-1138.
24. Miranda KC, Bond DT, McKee M, Skog J, Paunescu TG, Da Silva N, Brown D, Russo LM. Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney Int 2010; 78: 191-199.
25. Gonzales PA, Zhou H, Pisitkum T, et al. Isolation and Purification of Exosomes in Urine, Chapter 6. In: Rai AJ editor. The Urinary Proteome: Methods and Protocols, Methods in Molecular Biology. Springer; 2010; Volume 641, pp. 89-99.
26. Bobrie A, Colombo M, Krumeich S, Raposo G, Théry C. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles 2012; 1.
27. Stenzl A, Hennenlotter J, Schilling D. Can we still afford bladder cancer? Curr Opin Urol 2008; 18: 488-492.
28. Xie L, Chen X, Wang L, Qian X, Wang T, Wei J, Yu L, Ding Y, Zhang C, Liu B. Cell-free miRNAs may indicate diagnosis and docetaxel sensitivity of tumor cells in malignant effusions. BMC Cancer 2010; 10: 591.
29. Scheffer AR, Holdenrieder S, Kristiansen G, von Ruecker A, Müller SC, Ellinger J. Circulating microRNAs in serum: novel biomarkers for patients with bladder cancer? World J Urol 2014; 32: 353-358.
30. Hessvik NP, Sandvig K, Llorente A. Exosomal miRNAs as Biomarkers for Prostate Cancer. Front Genet 2013; 4: 36.