Microbial colonization of normal skin: Direct visualization of 194 skin biopsies

Anaerobe

Volumn 38, Issue , 2016, Pages 47-49

  Jahns, Anika C ^a   Alexeyev, Oleg A ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

Biopsy; Hair follicle; Immunofluorescence; P. acnes; Skin

Indexed keywords

ARTICLE; BACTERIAL COLONIZATION; BACTERIUM COLONY; BACTERIUM CONTAMINATION; BACTERIUM EXAMINATION; FLUORESCENCE ANALYSIS; HAIR FOLLICLE; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; PROPIONIBACTERIUM ACNES; SKIN BIOPSY; SPECIES DOMINANCE; STRATUM CORNEUM; ADOLESCENT; ADULT; AGED; BIOPSY; CHILD; CONFOCAL MICROSCOPY; FEMALE; MALE; MICROBIOLOGY; MICROFLORA; MIDDLE AGED; SKIN; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BIOPSY; CHILD; FEMALE; HAIR FOLLICLE; HUMANS; MALE; MICROBIOTA; MICROSCOPY, CONFOCAL; MIDDLE AGED; SKIN; YOUNG ADULT;

EID: 84949663627     PISSN: 10759964     EISSN: 10958274     Source Type: Journal    
DOI: 10.1016/j.anaerobe.2015.11.011     Document Type: Article

References (16)

1. Vartoukian S.R., Palmer R.M., Wade W.G. Strategies for culture of 'unculturable' bacteria. FEMS Microbiol. Lett. 2010, 309:1-7.
2. Hugenholtz P., Hooper S.D., Kyrpides N.C. Focus: Synergistetes. Environ. Microbiol. 2009, 11:1327-1329.
3. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 1995, 59:143-169.
4. Kong H.H., Segre J.A. Skin microbiome: looking back to move forward. J. Invest Dermatol 2012, 132:933-939.
5. Grice E.A., Kong H.H., Conlan S., Deming C.B., Davis J., Young A.C., et al. Topographical and temporal diversity of the human skin microbiome. Science 2009, 324:1190-1192.
6. Luna G.M., Manini E., Danovaro R. Large fraction of dead and inactive bacteria in coastal marine sediments: comparison of protocols for determination and ecological significance. Appl. Environ. Microbiol. 2002, 68:3509-3513.
7. Amann R., Moraru C. Two decades of fluorescence in situ hybridization in systematic and applied microbiology. Syst. Appl. Microbiol. 2012, 35:483-484.
8. Jahns A.C., Lundskog B., Berg J., Jonsson R., McDowell A., Patrick S., et al. Microbiology of folliculitis: a histological study of 39 cases. APMIS 2014, 122:25-32.
9. Jahns A.C., Lundskog B., Ganceviciene R., Palmer R.H., Golovleva I., Zouboulis C.C., et al. An increased incidence of Propionibacterium acnes biofilms in acne vulgaris: a case-control study. Br. J. Dermatology 2012, 167:50-58.
10. Kepner R.L., Pratt J.R. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present. Microbiol. Rev. 1994, 58:603-615.
11. Jahns A.C., Eilers H., Ganceviciene R., Alexeyev O.A. Propionibacterium species and follicular keratinocyte activation in acneic and normal skin. Br. J. Dermatology 2015, 172:981-987.
12. Jahns A.C., Oprica C., Vassilaki I., Golovleva I., Palmer R.H., Alexeyev O.A. Simultaneous visualization of Propionibacterium acnes and Propionibacterium granulosum with immunofluorescence and fluorescence in situ hybridization. Anaerobe 2013, 23:48-54.
13. Grice E.A., Segre J.A. The skin microbiome. Nat. Rev. Microbiol. 2011, 9:244-253.
14. Ben-Amor K., Heilig H., Smidt H., Vaughan E.E., Abee T., de Vos W.M. Genetic diversity of viable, injured, and dead fecal bacteria assessed by fluorescence-activated cell sorting and 16S rRNA gene analysis. Appl. Environ. Microbiol. 2005, 71:4679-4689.
15. Alexeyev O.A., Jahns A.C. Sampling and detection of skin Propionibacterium acnes: current status. Anaerobe 2012, 18:479-483.
16. Jahns A.C., Alexeyev O.A. Three dimensional distribution of Propionibacterium acnes biofilms in human skin. Exp. Dermatol. 2014, 23:687-689.