Isolation of mouse neutrophils

Current Protocols in Immunology

Volumn 2015, Issue , 2015, Pages 3.20.1-3.20.15

  Swamydas, Muthulekha ^a   Luo, Yi ^b   Dorf, Martin E ^b   Lionakis, Michail S ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Density gradient centrifugation; Isolation; Magnetic separation; Mouse bone marrow; Mouse tissue; Neutrophils; Sorting

Indexed keywords

ADOPTIVE TRANSFER; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD SAMPLING; BONE MARROW CELL; CANDIDA ALBICANS; CELL FUNCTION; CELL ISOLATION; CELL KILLING; CELL VIABILITY; CENTRIFUGATION; CLINICAL PROTOCOL; CONTROLLED STUDY; CYTOKINE PRODUCTION; DENSITY GRADIENT; EX VIVO STUDY; FLUORESCENCE ACTIVATED CELL SORTING; HOST PATHOGEN INTERACTION; IMMUNOLOGICAL PROCEDURES; IMMUNOMAGNETIC SELECTION; MALE; MOUSE; MOUSE MODEL; NEUTROPHIL; NEUTROPHIL CHEMOTAXIS; NONHUMAN; OXIDATIVE STRESS; PERITONEAL FLUID; PHAGOCYTOSIS; PRIORITY JOURNAL; ANIMAL; ASCITES FLUID; CELL SEPARATION; CYTOLOGY; METABOLISM; PROCEDURES;

ANIMALS; ASCITIC FLUID; BONE MARROW CELLS; CELL SEPARATION; MICE; NEUTROPHILS;

EID: 84949193497     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im0320s110     Document Type: Article

References (12)

1. Amulic, B., Cazalet, C., Hayes, G.L., Metzler, K.D., and Zychlinsky, A. 2012. Neutrophil function: From mechanisms to disease. Annu. Rev. Immunol 30:459-489.
2. Boxio, R., Bossenmeyer-Pourie, C., Steinckwich, N., Dournon, C., and Nusse, O. 2004. Mouse bone marrow contains large numbers of functionally competent neutrophils. J. Leukoc. Biol 75:604-611.
3. Clark, R.A. and Nauseef, W.M. 2001. Isolation and functional analysis of neutrophils. Curr. Protoc Immunol. 19:7.23.1-7.23.17.
4. Daley, J.M., Thomay, A.A., Connolly, M.D., Reichner, J.S., and Albina, J.E. 2008. Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J. Leukoc. Biol. 83:64-70.
5. Devi, S., Laning, J., Luo, Y., and Dorf, M.E. 1995 Biologic activities of the β-chemokine TCA3 on neutrophils and macrophages. J. Immunol 154:5376-5383.
6. Kruger, P., Saffarzadeh, M., Weber, A.N., Rieber, N., Radsak, M., von Bernuth, H., Benarafa, C., Roos, D., Skokowa, J., and Hartl, D. 2015 Neutrophils: Between host defence, immune modulation, and tissue injury. PLoS Pathog 11:e1004651.
7. Lionakis, M.S., Lim, J.K., Lee, C.C., and Murphy, P.M. 2011. Organ-specific innate immune responses in a mouse model of invasive candidiasis J. Innate Immun. 3:180-199.
8. Lionakis, M.S., Fischer, B.G., Lim, J.K., Swamydas, M., Wan, W., Richard Lee, C.C., Cohen, J.I., Scheinberg, P., Gao, J.L., and Murphy, P.M. 2012. Chemokine receptor Ccr1 drives neutrophil-mediated kidney immunopathology and mortality in invasive candidiasis. PLoS Pathog. 8:e1002865.
9. Luo, Y. and Dorf, M.E. 2001. Isolation of mouse neutrophils. Curr. Protoc. Immunol. 22:3.20.1-3.20.6.
10. Narasaraju, T., Yang, E., Samy, R.P., Ng, H.H., Poh, W.P., Liew, A.A., Phoon, M.C., van Rooijen, N., and Chow, V.T. 2011. Excessive neutrophils and neutrophil extracellular traps contribute to acute lung injury of influenza pneumonitis. Am J. Pathol. 179:199-210.
11. Watt, S.M., Burgess, A.W., and Metcalf, D. 1979 Isolation and surface labeling of murine polymorphonuclear neutrophils. J. Cell Physiol 100:1-21.
12. Whitney, P.G., Bar, E., Osorio, F., Rogers, N.C., Schraml, B.U., Deddouche, S., LeibundGut-Landmann, S., and Reis e Sousa, C. 2014. Syk signaling in dendritic cells orchestrates innate resistance to systemic fungal infection. PLoS Pathog. 10:e1004276.