Folding and purification of insoluble (inclusion body) proteins from Escherichia coli

Current Protocols in Protein Science

Volumn 2014, Issue , 2014, Pages 6.5.1-6.5.30

  Wingfield, Paul T ^a   Palmer, Ira ^a   Liang, Shu Mei ^b  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b AGRICULTURAL BIOTECHNOLOGY RESEARCH CENTER   (Taiwan)

Author keywords

Escherichia coli; Inclusion body; Protein folding; Protein purification; Recombinant protein

Indexed keywords

ACETIC ACID; GROWTH HORMONE; GUANIDINE; INTEGRASE; INTERLEUKIN 2; RECOMBINANT PROTEIN; THROMBIN; WATER;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL INCLUSION; DIALYSIS; DILUTION; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEDIMENTATION; SOLUBILIZATION; ANIMAL; CHEMISTRY; HUMAN; ISOLATION AND PURIFICATION; PROTEIN REFOLDING;

ANIMALS; ESCHERICHIA COLI; GUANIDINE; HUMANS; INCLUSION BODIES; PROTEIN DENATURATION; PROTEIN REFOLDING; RECOMBINANT PROTEINS;

EID: 84949189494     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0605s78     Document Type: Article

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