Functional analysis of the exocyst subunit Sec15 in Candida albicans

Eukaryotic Cell

Volumn 14, Issue 12, 2015, Pages 1228-1239

  Chavez Dozal, Alba A ^a,b   Bernardo, Stella M ^a,b   Rane, Hallie S ^a   Lee, Samuel A ^a,b  

^a NEW MEXICO VA HEALTH CARE SYSTEM   (United States)

^b UNIVERSITY OF NEW MEXICO HEALTH SCIENCES CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; CHITINASE; DOXYCYCLINE; FUNGAL PROTEIN; GREEN FLUORESCENT PROTEIN; HYDROLASE; PROTEIN SUBUNIT; TETRACYCLINE; TRIACYLGLYCEROL LIPASE; ZYMOLYASE;

ADHESION; CANDIDA ALBICANS; CELL WALL; DRUG EFFECTS; ERRATUM; FUNGUS HYPHAE; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MICROBIAL VIABILITY; MUTATION; PHENOTYPE; PROTEIN SUBUNIT; RETRACTED ARTICLE;

ADHESIVENESS; ASPARTIC ACID PROTEASES; CANDIDA ALBICANS; CELL WALL; CHITINASES; DOXYCYCLINE; FUNGAL PROTEINS; GREEN FLUORESCENT PROTEINS; HYDROLASES; HYPHAE; LIPASE; MICROBIAL VIABILITY; MUTATION; PHENOTYPE; PROTEIN SUBUNITS; TETRACYCLINE;

EID: 84949033900     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00147-15     Document Type: Article

References (34)

1. Heider MR, Munson M. 2012. Exorcising the exocyst complex. Traffic 13:898-907. http://dx.doi.org/10.1111/j.1600-0854.2012.01353.x.
2. TerBush DR, Maurice T, Roth D, Novick P. 1996. The exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15:6483-6494.
3. Novick P, Ferro S, Schekman R. 1981. Order of events in the yeast secretory pathway. Cell 25:461-469. http://dx.doi.org/10.1016/0092-8674(81)90064-7.
4. Li CR, Lee RT, Wang YM, Zheng XD, Wang Y. 2007. Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3pdependent phases separated by septin ring formation. J Cell Sci 120(Part 11):1898-1907. http://dx.doi.org/10.1242/jcs.002931.
5. Caballero-Lima D, Sudbery PE. 2014. In Candida albicans, phosphorylation of Exo84 by Cdk1-Hgc1 is necessary for efficient hyphal extension. Mol Biol Cell 25:1097-1110. http://dx.doi.org/10.1091/mbc.E13-11-0688.
6. Chavez-Dozal AA, Bernardo SM, Rane HS, Herrera G, Kulkarny V, Wagener J, Cunningham I, Brand AC, Gow NA, Lee SA. 2015. The Candida albicans exocyst subunit Sec6 contributes to cell wall integrity and is a determinant of hyphal branching. Eukaryot Cell 14:684-697. http://dx.doi.org/10.1128/EC.00028-15.
7. Novick P, Field C, Schekman R. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21:205-215. http://dx.doi.org/10.1016/0092-8674(80)90128-2.
8. France YE, Boyd C, Coleman J, Novick PJ. 2006. The polarityestablishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. J Cell Sci 119:876-888. http://dx.doi.org/10.1242/jcs.02849.
9. Aronov S, Gerst JE. 2004. Involvement of the late secretory pathway in actin regulation and mRNA transport in yeast. J Biol Chem 279:36962-36971. http://dx.doi.org/10.1074/jbc.M402068200.
10. Bernardo SM, Rane HS, Chavez-Dozal A, Lee SA. 2014. Secretion and filamentation are mediated by the Candida albicans t-SNAREs Sso2p and Sec9p. FEMS Yeast Res 14:762-775. http://dx.doi.org/10.1111/1567-1364.12165.
11. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403-410. http://dx.doi.org/10.1016/S0022-2836(05)80360-2.
12. Bernardo SM, Khalique Z, Kot J, Jones JK, Lee SA. 2008. Candida albicans VPS1 contributes to protease secretion, filamentation, and biofilm formation. Fungal Genet Biol 45:861-877. http://dx.doi.org/10.1016/j.fgb.2008.01.001.
13. Wilson RB, Davis D, Enloe BM, Mitchell AP. 2000. A recyclable Candida albicans URA3 cassette for PCR product-directed gene disruptions. Yeast 16:65-70. http://dx.doi.org/10.1002/(SICI)1097-0061(20000115)16:1<65::AID-YEA5083.0.CO;2-M.
14. Bates S, Hughes HB, Munro CA, Thomas WP, McCallum DM, Bertram G, Atrih A, Fergunson MA, Brown AJ, Odds FC, Gow NA. 2006. Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans. J Biol Chem 281:90-98. http://dx.doi.org/10.1074/jbc.M510360200.
15. Nakayama H, Mio T, Nagahashi S, Kokado M, Arisawa M, Aoki Y. 2000. Tetracycline-regulatable system to tightly control gene expression in the pathogenic fungus Candida albicans. Infect Immun 68:6712-6719. http://dx.doi.org/10.1128/IAI.68.12.6712-6719.2000.
16. Rane HS, Bernardo SM, Raines SM, Binder JL, Parra KJ, Lee SA. 2013. Candida albicans VMA3 is necessary for V-ATPase assembly and function and contributes to secretion and filamentation. Eukaryot Cell 12:1369-1382. http://dx.doi.org/10.1128/EC.00118-13.
17. Kashiwabuchi RT, Carvalho FR, Khan YA, Hirai F, Campos MS, McDonnell PJ. 2013. Assessment of fungal viability after long-wave ultraviolet light irradiation combined with riboflavin administration. Graefes Arch Clin Exp Ophthalmol 251:521-527. http://dx.doi.org/10.1007/s00417-012-2209-z.
18. Tran SL, Puhar A, Ngo-Camus M, Ramarao N. 2011. Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus. PLoS One 6(9):e22876. http://dx.doi.org/10.1371/journal.pone.0022876.
19. Bernardo SM, Lee SA. 2010. Candida albicans SUR7 contributes to secretion, biofilm formation, and macrophage killing. BMC Microbiol 10:133. http://dx.doi.org/10.1186/1471-2180-10-133.
20. Imbert C, Rodier MH, Daniault G, Jacquemin JL. 2002. Influence of sub-inhibitory concentrations of conventional antifungals on metabolism of Candida albicans and on its adherence to polystyrene and extracellular matrix proteins. Med Mycol 40:123-129.
21. Pierce CG, Uppuluri P, Tummala S, Lopez-Ribot JL. 2010. A 96 well microtiter plate-based method for monitoring formation and antifungal susceptibility testing of Candida albicans biofilms. J Vis Exp 2010(44):pii: 2287. http://dx.doi.org/10.3791/2287.
22. Lee SA, Mao Y, Zhang Z, Wong B. 2001. Overexpression of a dominantnegative allele of YPT1 inhibits growth and aspartyl protease secretion in Candida albicans. Microbiology 147:1961-1970. http://dx.doi.org/10.1099/00221287-147-7-1961.
23. Kretschmar M, Felk A, Staib P, Hess D, Callapina M, Morschhauser J, Schafer W, Korting HC, Hof H, Hube B, Nichterlein T. 2002. Individual acid aspartic proteinases (Saps) 1-6 of Candida albicans are not essential for invasion and colonization of the gastrointestinal tract in mice. Microb Pathog 32:61-70. http://dx.doi.org/10.1006/mpat.2001.0478.
24. von Tigerstrom RG, Stelmaschuk S. 1989. The use of Tween 20 in a sensitive turbidimetric assay of lipolytic enzymes. Can J Microbiol 35:511-514. http://dx.doi.org/10.1139/m89-079.
25. Sudbery PE. 2001. The germ tubes of Candida albicans hyphae and pseudohyphae show different patterns of septin ring localization. Mol Microbiol 41:19-31. http://dx.doi.org/10.1046/j.1365-2958.2001.02459.x.
26. Milne SW, Cheetham J, Lloyd D, Aves S, Bates S. 2011. Cassettes for PCR-mediated gene tagging in Candida albicans utilizing nourseothricin resistance. Yeast 28:833-841. http://dx.doi.org/10.1002/yea.1910.
27. Kapteyn JC, Ram AF, Groos EM, Kollar R, Montijn RC, Van Den Ende H, Llobell A, Cabib E, Klis FM. 1997. Altered extent of cross-linking of beta-1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta-1,3-glucan content. J Bacteriol 179: 6279-6284.
28. Chandra J, Kuhn DM, Mukherjee PK, Hoyer LL, McCormic T, Ghannoum MA. 2001. Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance. J Bacteriol 183: 5385-5394. http://dx.doi.org/10.1128/JB.183.18.5385-5394.2001.
29. Merkel GJ. 1992. Effects of culture conditions on the in vitro infection of fibroblasts by Candida albicans. Can J Microbiol 38:135-142. http://dx.doi.org/10.1139/m92-022.
30. Cutler JE, Brawner DL, Hazen KC, Jutila MA. 1990. Characteristics of Candida albicans adherence to mouse tissues. Infect Immun 58:1902-1908.
31. King RD, Lee JC, Morris AL. 1980. Adherence of Candida albicans and other Candida species to mucosal epithelial cells. Infect Immun 27:667-674.
32. Jones LA, Sudbery PE. 2010. Spitzenkorper, exocyst, and polarisome components in Candida albicans hyphae show different patterns of localization and have distinct dynamic properties. Eukaryot Cell 9:1455-1465. http://dx.doi.org/10.1128/EC.00109-10.
33. Sullivan PA, Yin CW, Molloy C. 1983. An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation. Can J Microbiol 29:1514-1525. http://dx.doi.org/10.1139/m83-233.
34. Sivaram MV, Saporita JA, Furgason MLM, Boettcher AJ, Munson M. 2005. Dimerization of the exocyst protein Sec6p and its interaction with the t-SNARE Sec9p. Biochemistry 44:6302-6311. http://dx.doi.org/10.1021/bi048008z.