Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

Genome Biology

Volumn 16, Issue 1, 2015, Pages

  Karvelis, Tautvydas ^a   Gasiunas, Giedrius ^a   Young, Joshua ^b   Bigelyte, Greta ^a   Silanskas, Arunas ^a   Cigan, Mark ^b   Siksnys, Virginijus ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

^b DUPONT PIONEER   (United States)

Author keywords

Cas9; CRISPR; Guide RNA determination; Nuclease; PAM determination

Indexed keywords

ADENINE; AMINO ACID; ASPARAGINYLASPARAGINYLALANYLGLYCYLALANYLALANYLTRYPTOPHAN; ASPARAGINYLASPARAGINYLASPARAGINYLASPARAGINYLCYSTEINYLASPARAGINYLASPARTIC ACID; ASPARAGINYLGLYCYLGLYCINE; ASPARAGINYLGLYCYLGLYCYLASPARAGINYLCYSTEINYLASPARAGINYLASPARTYLASPARTIC ACID; ASPARAGINYLGLYCYLGLYCYLASPARAGINYLGLYCINE; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; CRISPR ASSOCIATED PROTEIN; CYTOSINE; GUANINE; GUIDE RNA; LYSYLARGINYLPHENYLALANYLARGINYLASPARTYLARGINYLHISTIDINYLASPARTYLGLYCYLGLUTAMYLLEUCYLGLYCYLGLYCYLARGINYLLYSYLARGINYLALANYLARGININE; METHIONYLALANYLPROPYLLYSYLLYSYLLYSYLARGINYLLYSYLVALINE; PROTEIN CAS9; PROTEIN CRISPR1; PROTEIN CRISPR3; SINGLE GUIDE RNA; THYMINE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENOME; BREVIBACILLUS; BREVIBACILLUS LATEROSPORUS; CONTROLLED STUDY; CRISPR CAS SYSTEM; DNA MODIFICATION; DNA SEQUENCE; GENE LIBRARY; GENE TARGETING; IN VITRO STUDY; MAIZE; NONHUMAN; PLASMID; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN FUNCTION; PROTEIN MOTIF; PROTOSPACER ADJACENT MOTIF; RNA SEQUENCE; SEQUENCE ANALYSIS; STRAIN DIFFERENCE; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS THERMOPHILUS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES; STREPTOCOCCUS;

BACTERIAL PROTEINS; BASE SEQUENCE; BREVIBACILLUS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; ENDONUCLEASES; ESCHERICHIA COLI; GENE TARGETING; MOLECULAR SEQUENCE DATA; RNA, GUIDE; STREPTOCOCCUS;

EID: 84947730555     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-015-0818-7     Document Type: Article

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