DESI–MS as a tool for direct lipid analysis in cultured cells

Cytotechnology

Volumn 67, Issue 6, 2014, Pages 1085-1091

  Bodzon Kulakowska, Anna ^a   Cichon, Tomasz ^a   Golec, Agnieszka ^a   Drabik, Anna ^a   Ner, Joanna ^a   Suder, Piotr ^a  

^a AGH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Poland)

Author keywords

Cell culture; Desorption electrospray ionization (DESI); Lipid analysis; Oxidative stress

Indexed keywords

LIPID;

3T3 CELL LINE; ANALYTIC METHOD; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DESORPTION ELECTROSPRAY IONIZATION MASS SPECTROMETRY; ELECTROSPRAY MASS SPECTROMETRY; LIPID ANALYSIS; MONOLAYER CULTURE; OXIDATIVE STRESS;

EID: 84946484652     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-014-9734-z     Document Type: Article

References (15)

1. Bodzon-Kulakowska A, Drabik A, Ner J, Kotlinska JH, Suder P (2014) Desorption electrospray ionisation (DESI) for beginners—how to adjust settings for tissue imaging. Rapid Commun Mass Spectrom 28:1–9
2. Eberlin LS, Ferreira CR, Dill AL, Ifa DR, Cooks RG (2011) Desorption electrospray ionization mass spectrometry for lipid characterization and biological tissue imaging. Biochim Biophys Acta 1811:946–960
3. Feeney CJ, Frantseva MV, Carlen PL, Pennefather PS, Shulyakova N, Shniffer C, Mills LR (2008) Vulnerability of glial cells to hydrogen peroxide in cultured hippocampal slices. Brain Res 1198:1–15
4. Gille JJP, Joenje H (2002) Cell culture models for oxidative stress: superoxide and hydrogen peroxide versus normobaric hyperoxia. Mutat Res 275:405–414
5. Girod M, Cheng J, Cooks R, Shi Y (2010a) Mapping lipid alterations in traumatically injured rat spinal cord by desorption electrospray ionization imaging mass spectrometry. Anal Chem 83:207–215
6. Girod M, Shi Y, Cheng J-X, Cooks RG (2010b) Desorption electrospray ionization imaging mass spectrometry of lipids in rat spinal cord. J Am Soc Mass Spectrom 21:1177–1189
7. Hsu F–F, Turk J (2005) Studies on phosphatidylserine by tandem quadrupole and multiple stage quadrupole ion-trap mass spectrometry with electrospray ionization: structural characterization and the fragmentation processes. J Am Soc Mass Spectrom 16:1510–1522
8. Hsu F–F, Turk J (2009) Electrospray ionization with low-energy collisionally activated dissociation tandem mass spectrometry of glycerophospholipids: mechanisms of fragmentation and structural characterization. J Chromatogr B 877:2673–2695
9. Kiyoshima T, Enoki N, Kobayashi I, Sakai T, Nagata K, Wada H, Fujiwara H, Ookuma Y, Sakai H (2012) Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts. Int J Mol Med 30:1007–1012
10. Laskin J, Heath BS, Roach PJ, Cazares L, Semmes OJ (2012) Tissue imaging using nanospray desorption electrospray ionization mass spectrometry. Anal Chem 84:141–148
11. Manicke NE, Wiseman JM, Ifa DR, Cooks RG (2008) Desorption electrospray ionization (DESI) mass spectrometry and tandem mass spectrometry (MS/MS) of phospholipids and sphingolipids: ionization, adduct formation, and fragmentation. J Am Soc Mass Spectrom 19:531–543
12. Patterson MK Jr (1979) Measurement of growth and viability of cells in culture. Methods Enzymol 58:141–152
13. Piwowar AM, Keskin S, Delgado MO, Shen K, Hue JJ, Lanekoff I, Ewing AG, Winograd N (2013) C60-ToF SIMS imaging of frozen hydrated HeLa cells. Surf Interface Anal 45:302–304
14. Takáts Z, Wiseman JM, Gologan B, Cooks RG (2004) Mass spectrometry sampling under ambient conditions with desorption electrospray ionization. Science 306:471–473
15. Weismann D, Binder CJ (2012) The innate immune response to products of phospholipid peroxidation. Biochim Biophys Acta 1818:2465–2475