Monitoring chitin deposition during septum assembly in budding yeast

Methods in Molecular Biology

Volumn 1369, Issue , 2016, Pages 59-72

  Arcones, Irene ^a   Roncero, Cesar ^a  

^a NONE

Author keywords

Chitin; Chitin ring; Chitin synthase; Primary septum

Indexed keywords

CHITIN SYNTHASE; CHITIN;

CONTROLLED STUDY; CYTOKINESIS; ENZYME ACTIVITY; FLUORESCENCE MICROSCOPY; NONHUMAN; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; CELL WALL; METABOLISM; PHYSIOLOGY; PROCEDURES; SACCHAROMYCETALES;

CELL WALL; CHITIN; CHITIN SYNTHASE; CYTOKINESIS; MICROSCOPY, FLUORESCENCE; SACCHAROMYCETALES;

EID: 84946433651     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3145-3_5     Document Type: Chapter

References (28)

1. Cabib E (2004) The septation apparatus, a chitin-requiring machine in budding yeast. Arch Biochem Biophys 426:201–207
2. Cabib E, Arroyo J (2013) How carbohydrates sculpt cells: chemical control of morphogenesis in the yeast cell wall. Nat Rev Microbiol 11: 648–655
3. Roncero C, Sanchez Y (2010) Cell separation and the maintenance of cell integrity during cytokinesis in yeast: the assembly of a septum. Yeast 27:521–530
4. Roncero C, Valdivieso MH, Ribas JC, Duran A (1988) Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white. J Bacteriol 170:1950–1954
5. Shaw JA, Mol PC, Bowers B, Silverman SJ, Valdivieso MH, Duran A, Cabib E (1991) The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J Cell Biol 114:111–123
6. Cabib E, Schmidt M (2003) Chitin synthase III activity, but not the chitin ring, is required for remedial septa formation in budding yeast. FEMS Microbiol Lett 29:299–305
7. Gomez A, Perez J, Reyes A, Duran A, Roncero C (2009) Slt2 and Rim101 contribute independently to the correct assembly of the chitin ring at the budding yeast neck in Saccharomycces cerevisiae. Eukaryot Cell 8:1449–1559
8. Schmidt M, Bowers B, Varma A, Roh DH, Cabib E (2002) In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other. J Cell Sci 115:293–302
9. VerPlank L, Li R (2005) Cell cycle-regulated trafficking of Chs2 controls actomyosin ring stability during cytokinesis. Mol Biol Cell 16:2529–2543
10. Cabib E, Sburlati A, Bowers B, Silverman SJ (1989) Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J Cell Biol 108:1665–1672
11. Duran A, Bowers B, Cabib E (1975) Chitin synthase zymogen is attached to the plasma membrane. Proc Natl Acad Sci U S A 72: 3952–3955
12. Roncero C (2002) The genetic complexity of chitin synthesis in fungi. Curr Genet 41: 367–378
13. Pringle JR (1991) Staining of bud scars and other cell wall chitin with calcofluor. Methods Enzymol 194:732–735
14. Roncero C, Duran A (1985) Effect of Calcofluor white and Congo red on fungal wall morphogenesis: in vivo activation of chitin polymerization. J Bacteriol 163:1180–1185
15. Reyes A, Sanz M, Duran A, Roncero C (2007) Chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane. J Cell Sci 120:1998–2009
16. Sanz M, Castrejon F, Duran A, Roncero C (2004) Saccharomyces cerevisiae Bni4p directs the formation of the chitin ring and also participates in the correct assembly of the septum structure. Microbiology 150:3229–3241
17. Oh Y, Chang KJ, Orlean P, Wloka C, Deshaies R, Bi E (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23:2445–2456
18. Keller FA, Cabib E (1971) Chitin and yeast budding. Properties of chitin synthetase from Saccharomyces carlsbergensis. J Biol Chem 10:160–166
19. Choi WJ, Cabib E (1994) The use of divalent cations and pH for the determination of specific yeast chitin synthetases. Anal Biochem 219:368–372
20. Orlean P (1987) Two chitin synthases in Saccharomyces cerevisiae. J Biol Chem 262: 368–372
21. Cabib E, Farkas V (1971) The control of morphogenesis: an enzymatic mechanism for the initiation of septum formation in yeast. Proc Natl Acad Sci U S A 68:2052–2056
22. Lucero HA, Kuranda MJ, Bulik DA (2002) A nonradioactive, high throughput assay for chitin synthase activity. Anal Biochem 305: 97–105
23. Magellan H, Boccara M, Drujon T, Soulié MC, Guillou C, Dubois J, Becker HF (2013) Discovery of two new inhibitors of Botrytis cinerea chitin synthase by a chemical library screening. Bioorg Med Chem 21:4997–5003
24. Jimenez C, Sacristan C, Roncero MI, Roncero C (2010) Amino acid divergence between the CHS domain contributes to the different intracellular behaviour of Family II fungal chitin synthases in Saccharomyces cerevisiae. Fungal Genet Biol 47:1034–1043
25. Devrekanli A, Foltman M, Roncero C, Sanchez-Diaz A, Labib K (2012) Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts. J Cell Sci 125: 5453–5466
26. Fernandez MP, Correa JU, Cabib E (1982) Activation of chitin synthetase in permeabilized cells of a Saccharomyces cerevisiae mutant lacking proteinase B. J Bacteriol 152:1255–1264
27. Crotti LB, Drgon T, Cabib E (2001) Yeast cell permeabilization by osmotic shock allows determination of enzymatic activities in situ. Anal Biochem 292:8–16
28. Valdivia RH, Schekman R (2003) The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane. Proc Natl Acad Sci U S A 100:10287–10292