One-step multiplex PCR assay for differentiating proposed new species "clostridium neonatale" from closely related species

Journal of Clinical Microbiology

Volumn 53, Issue 11, 2015, Pages 3621-3623

  Ferraris, Laurent ^a   Schönherr, Sophia ^a   Bouvet, Philippe ^b   Dauphin, Brunhilde ^c   Popoff, Michel ^b   Butel, Marie Jose ^a   Aires, Julio ^a  

^a DHU Risques et Grossesse ^*  (France)

^b INSTITUT PASTEUR   (France)

^c Andromas SAS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; RNA 16S; PRIMER DNA;

ARTICLE; BACTERIAL COLONIZATION; BACTERIAL STRAIN; BACTERIUM ISOLATE; CLOSTRIDIUM; CLOSTRIDIUM BEIJERINCKII; CLOSTRIDIUM BUTYRICUM; CLOSTRIDIUM NEONATALE; FECES ANALYSIS; GENE SEQUENCE; HUMAN; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MULTIPLEX POLYMERASE CHAIN REACTION; NECROTIZING ENTEROCOLITIS; NEW SPECIES; NEWBORN; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA GENE; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENTIATION; CANADA; CLASSIFICATION; CROSS INFECTION; ENTEROCOLITIS, NECROTIZING; EPIDEMIC; FECES; GENETICS; INTESTINE; ISOLATION AND PURIFICATION; MATRIX-ASSISTED LASER DESORPTION-IONIZATION MASS SPECTROMETRY; MICROBIOLOGY; NEONATAL INTENSIVE CARE UNIT; PROCEDURES;

CANADA; CLOSTRIDIUM; CROSS INFECTION; DISEASE OUTBREAKS; DNA PRIMERS; DNA, BACTERIAL; ENTEROCOLITIS, NECROTIZING; FECES; HUMANS; INFANT, NEWBORN; INTENSIVE CARE UNITS, NEONATAL; INTESTINES; MULTIPLEX POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84945182575     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01404-15     Document Type: Article

References (14)

1. Alfa MJ, Robson D, Davi M, Bernard K, Van Caeseele P, Harding GK. 2002. An outbreak of necrotizing enterocolitis associated with a novel Clostridium species in a neonatal intensive care unit. Clin Infect Dis 35: S101-S105. http://dx.doi.org/10.1086/341929.
2. Bouvet P, Ferraris L, Dauphin B, Popoff MR, Butel MJ, Aires J. 2014. 16S rRNA gene sequencing, multilocus sequence analysis, and mass spec-trometry identification of the proposed new species "Clostridium neona-tale." J Clin Microbiol 52:4129-4136. http://dx.doi.org/10.1128/JCM.00477-14.
3. Howard FM, Bradley JM, Flynn DM, Noone P, Szawatkowski M. 1977. Outbreak of necrotising enterocolitis caused by Clostridium butyricum. Lancet ii:1099-1102.
4. Mitchell RG, Etches PC, Day DG. 1981. Non-toxigenic clostridia in babies. J Clin Pathol 34:217-220. http://dx.doi.org/10.1136/jcp.34.2.217.
5. Kliegman RM, Fanaroff AA. 1984. Necrotizing enterocolitis. N Engl J Med 310:1093-1103. http://dx.doi.org/10.1056/NEJM198404263101707.
6. Sturm R, Staneck JL, Stauffer LR, Neblett WW, III. 1980. Neonatal necrotizing enterocolitis associated with penicillin-resistant, toxigenic Clostridium butyricum. Pediatrics 66:928-931.
7. Smith B, Bode S, Petersen BL, Jensen TK, Pipper C, Kloppenborg J, Boyé M, Krogfelt KA, Mølbak L. 2011. Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis. BMC Microbiol 11:73. http://dx.doi.org/10.1186/1471-2180-11-73.
8. Popoff MR, Szylit O, Ravisse P, Dabard J, Ohayon H. 1985. Experimental cecitis in gnotoxenic chickens monoassociated with Clostridium butyricum strains isolated from patients with neonatal necrotizing entero-colitis. Infect Immun 47:697-703.
9. Bousseboua H, Le Coz Y, Dabard J, Szylit O, Raibaud P, Popoff MR, Ravisse P. 1989. Experimental cecitis in gnotobiotic quails monoassoci-ated with Clostridium butyricum strains isolated from patients with neonatal necrotizing enterocolitis and from healthy newborns. Infect Immun 57:932-936.
10. Waligora-Dupriet AJ, Dugay A, Auzeil N, Huerre M, Butel MJ. 2005. Evidence for clostridial implication in experimental necrotising enteroco-litis through bacterial fermentation in a gnotobiotic quail model. Pediatr Res 58:629-635.
11. Szylit O, Butel MJ, Rimbault A. 1997. An experimental model of necro-tising enterocolitis. Lancet 350:33-34. http://dx.doi.org/10.1016/S0140-6736(05)66243-5.
12. Azcarate-Peril MA, Foster DM, Cadenas MB, Stone MR, Jacobi SK, Stauffer SH, Pease A, Gookin JL. 2011. Acute necrotizing enterocolitis of preterm piglets is characterized by dysbiosis of ileal mucosa-associated bacteria. Gut Microbes 2:234-243. http://dx.doi.org/10.4161/gmic.2.4.16332.
13. Ferraris L, Butel MJ, Campeotto F, Vodovar M, Rozé JC, Aires J. 2012. Clostridia in premature neonates' gut: incidence, antibiotic susceptibility, and perinatal determinants influencing colonization. PLoS One 7:e30594. http://dx.doi.org/10.1371/journal.pone.0030594.
14. Magot M, Carlier JP, Popoff MR. 1983. Identification of Clostridium butyricum and Clostridium beijerinckii by gas-liquid chromatography and sugar fermentation: correlation with DNA homologies and electropho-retic patterns. J Gen Microbiol 129:2837-2845.