Splicing by overlap extension pcr to obtain hybrid dna products

Methods in Molecular Biology

Volumn 1373, Issue , 2016, Pages 43-49

  Thornton, Justin A ^a  

^a NONE

Author keywords

Gene splicing; Mutagenesis; PCR fusion; Polymerase chain reaction

Indexed keywords

DNA HYBRID; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; CLONING VECTOR; DNA ISOLATION; DNA SEQUENCE; DNA SPLICING; DNA TEMPLATE; GENETIC MANIPULATION; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; TEMPERATURE; GENE VECTOR; GENETICS; HUMAN; NUCLEIC ACID HYBRIDIZATION; PATHOGENICITY; PROCEDURES; RNA SPLICING; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS;

DNA PRIMERS; GENETIC VECTORS; HUMANS; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA SPLICING; STAPHYLOCOCCUS;

EID: 84944930573     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/7651_2014_182     Document Type: Chapter

References (4)

1. Saiki RK, Scharf S, Faloona F et al (1985) Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354
2. Mullis K, Faloona F, Scharf S et al (1986) Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harb Symp Quant Biol 51(Pt 1):263-273
3. Ho SN, Hunt HD, Horton RM et al (1989) Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59
4. Horton RM, Hunt HD, Ho SN et al (1989) Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 77:61-68