High-Resolution Estimation of Multiple Cell Populations in Tissue Using Confocal Stereology

Fluorescence Microscopy: Super-Resolution and other Novel Techniques

Volumn , Issue , 2014, Pages 171-184

  Peterson, Daniel A ^a  

^a ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE   (United States)

Author keywords

Coefficient of error; Length; Number; Probe; Region of interest; Resolution; Sampling; Software; Virtual tissue

Indexed keywords

EID: 84943413261     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-12-409513-7.00012-9     Document Type: Chapter

References (8)

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2. Gundersen H.J., Bagger P., Bendtsen T.F., Evans S.M., Korbo L., Marcussen N., et al. The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 1988, 96(10):857-881.
3. Peterson D.A. The use of fluorescent probes in cell counting procedures. Quantitative Methods in Neuroscience: A Neuroanatomical Approach 2004, 85-114. Oxford University Press, Oxford. S.M. Evans, A.M. Janson, J.R. Nyengaard (Eds.).
4. West M.J. Basic Stereology for Biologists and Neuroscientists 2012, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
5. Sterio D.C. The unbiased estimation of number and sizes of arbitrary particles using the disector. J Microsc 1984, 134(2):127-136.
6. West M.J. New stereological methods for counting neurons. Neurobiol Aging 1993, 14(4):275-285.
7. Schmitz C., Hof P.R. Recommendations for straightforward and rigorous methods of counting neurons based on a computer simulation approach. J Chem Neuroanat 2000, 20(1):93-114.
8. West M.J., Slomianka L., Gundersen H.J. Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat Rec 1991, 231:482-497.